Complete Genome Sequence of <i>Bacillus subtilis</i> CNBG-PGPR-1 for Studying the Promotion of Plant Growth

Lu, Shanfa; Feng, Liuchun; Zhou, Dongqin; Jia, Mingyun; Liu, Zhuangzhuang; Hou, Zhaoqi; Li, Qi; Yu, Jinping

doi:10.1094/mpmi-07-22-0147-a

Cited by 4 publications

(3 citation statements)

References 26 publications

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“…In our previous study, the genome of CNBG‐PGPR‐1 was reported, and a series of genes related to plant growth promotion were mined (Lu et al., 2022). In the present study, we found the beneficial effect of CNBG‐PGPR‐1 on salt tolerance by regulating ethylene pathways in plants.…”

Section: Resultsmentioning

confidence: 99%

“…The strain CNBG‐PGPR‐1 was deposited in the China General Microbiological Culture Collection Center (CGMCC No. 19564) (Lu et al., 2022). In this study, strain CNBG‐PGPR‐1 was stored in 80% glycerol at −80°C.…”

Section: Methodsmentioning

confidence: 99%

“…Therefore, it is necessary to improve the salt tolerance of tomato through effective methods. In a previous study, Bacillus subtilis strain CNBG‐PGPR‐1, which has plant growth‐promoting properties, was isolated from naturally fermented organic fertilizer (Lu et al., 2022). Here, we found that CNBG‐PGPR‐1 could effectively mitigate the damage caused by salt stress in tomato.…”

Section: Introductionmentioning

confidence: 99%

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B. subtilisCNBG‐PGPR‐1 induces methionine to regulate ethylene pathway and ROS scavenging for improving salt tolerance of tomato

Feng,

Li,

Zhou

et al. 2023

The Plant Journal

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SUMMARYSoil salinity severely threatens plant growth and crop yields. The utilization of PGPR is an effective strategy for enhancing plant salt tolerance, but the mechanisms involved in this process have rarely been reported. In this study, we investigated the effects of Bacillus subtilis CNBG‐PGPR‐1 on improving plant salt tolerance and elucidated the molecular pathways involved. The results showed that CNBG‐PGPR‐1 significantly improved the cellular homeostasis and photosynthetic efficiency of leaves and reduced ion toxicity and osmotic stress caused by salt in tomato. Transcriptome analysis uncovered that CNBG‐PGPR‐1 enhanced plant salt tolerance through the activation of complex molecular pathways, with plant hormone signal transduction playing an important role. Comparative analysis and pharmacological experiments confirmed that the ethylene pathway was closely related to the beneficial effect of CNBG‐PGPR‐1 on improving plant salt tolerance. Furthermore, we found that methionine, a precursor of ethylene synthesis, significantly accumulated in response to CNBG‐PGPR‐1 in tomato. Exogenous L‐methionine largely mimicked the beneficial effects of CNBG‐PGPR‐1 and activated the expression of ethylene pathway‐related genes, indicating CNBG‐PGPR‐1 induces methionine accumulation to regulate the ethylene pathway in tomato. Finally, CNBG‐PGPR‐1 reduced salt‐induced ROS by activating ROS scavenger‐encoding genes, mainly involved in GSH metabolism and POD‐related genes, which were also closely linked to methionine metabolism. Overall, our studies demonstrate that CNBG‐PGPR‐1‐induced methionine is a key regulator in enhancing plant salt tolerance through the ethylene pathway and ROS scavenging, providing a novel understanding of the mechanism by which beneficial microbes improve plant salt tolerance.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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B. subtilisCNBG‐PGPR‐1 induces methionine to regulate ethylene pathway and ROS scavenging for improving salt tolerance of tomato

Feng,

Li,

Zhou

et al. 2023

The Plant Journal

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Bacillus spp.: Nature’s Gift to Agriculture and Humankind

Vishwakarma,

Ilyas,

Shahid

et al. 2024

Microorganisms for Sustainability

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Antagonistic Mechanism Analysis of Bacillus velezensis JLU-1, a Biocontrol Agent of Rice Pathogen Magnaporthe oryzae

Su,

Zhang,

Fan

et al. 2024

J. Agric. Food Chem.

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Magnaporthe oryzae, the causal agent of rice blast, is a fungal disease pathogen. Bacillus spp. have emerged as the most promising biological control agent alternative to chemical fungicides. In this study, the bacterial strain JLU-1 with significant antagonistic activity isolated from the rhizosphere soil of rice was identified as Bacillus velezensis through whole-genome sequencing, average nucleotide identity analysis, and 16S rRNA gene sequencing. Twelve gene clusters for secondary metabolite synthesis were identified in JLU-1. Furthermore, 3 secondary metabolites were identified in JLU-1, and the antagonistic effect of secondary metabolites against fungal pathogens was confirmed. Exposure to JLU-1 reduced the virulence of M. oryzae, and JLU-1 has the ability to induce the reactive oxygen species production of rice and improve the salt tolerance of rice. All of these results indicated that JLU-1 and its secondary metabolites have the promising potential to be developed into a biocontrol agent to control fungal diseases.

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Complete Genome Sequence of Bacillus subtilis CNBG-PGPR-1 for Studying the Promotion of Plant Growth

Cited by 4 publications

References 26 publications

B. subtilisCNBG‐PGPR‐1 induces methionine to regulate ethylene pathway and ROS scavenging for improving salt tolerance of tomato

B. subtilisCNBG‐PGPR‐1 induces methionine to regulate ethylene pathway and ROS scavenging for improving salt tolerance of tomato

Bacillus spp.: Nature’s Gift to Agriculture and Humankind

Antagonistic Mechanism Analysis of Bacillus velezensis JLU-1, a Biocontrol Agent of Rice Pathogen Magnaporthe oryzae

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