Complete genome sequence ofVibrio mimicusstrain SCCF01 with potential application in fish vaccine development

Abstract

Vibrio mimicus strain SCCF01 was isolated from the yellow catfish (Pelteobagrus fulvidraco) during a mass mortality event at an aquaculture facility. We sequenced the genome of strain SCCF01, then predicted possible effective targets for subunit vaccine and identified possible virulence genes for live attenuated vaccine. The genome of SCCF01 consists of 2 circular chromosomes of 3,213,040 and 1,272,975 bp with G+C content of 46.61% and 45.88%, respectively. Subcellular localization, number of transmembrane helices and adhesion probability were predicted by Vaxign programs. Several candidate virulence genes were identified using the Virulence Factors Database. The genomic information presented herein will be useful for future studies investigating the pathogenesis of V. mimicus and with vaccine development.Vibrio mimicus is an enteric pathogen that is widely distributed in aquatic environments that can infect and cause disease in fish.1,2 Additionally, the pathogen is zoonotic and can cause bacterial gastroenteritis and food poisoning in humans if infected fish are consumed.2 Hence, understanding the pathogenesis of V. mimicus and developing a vaccine to treat infected fish hosts is an important research direction. In this study, we revived V. mimicus strain SCCF01 from the farmed yellow catfish (Pelteobagrus fulvidraco) in Sichuan Province, China, during a mass mortality event.3 Our objectives were to: (1) characterize the SCCF01 genome, (2) predict possible effective targets for subunit vaccine, (3) identify possible virulence genes that could lead to development of attenuated V. mimicus for vaccine development, and (4) identify the ability of colonization and adhesion of V. mimicus infection in yellow catfish.The whole-genome sequence of SCCF01 strain was determined with single molecule real-time (SMRT) sequencing platform PacBio RS II. Single SMRT cells produced 35,089 reads for 306,431,068 bases (N50 size 12,135 bp and mean read length 8,732 bp). Subsequently, the sequence reads were assembled using the PacBio SMRT Analysis (ver. 2.3.0) with default options.

Complete genome analysis of Vibrio mimicus strain SCCF01, a highly virulent isolate from the freshwater catfish

“…Challenges showed that bath immersion of strain SCCF01 (10 [6] CFU·mL −1 ) caused 100% mortality of yellow catfish. The whole-genome sequence of V. mimicus was obtained by single-molecule real-time (SMRT) sequencing using platform PacBio RS II [12]. The complete genomic sequences of SCCF01 have been deposited in GenBank under the accession numbers CP016383 (chromosome I) and CP016384 (chromosome II).…”

“…Since 2011, large-scale V. mimicus infectious outbreaks have occurred continuously in Siluriformes farms in China. V. mimicus strain SCCF01, which is a highly virulent strain isolated from yellow catfish (Pelteobagrus fulvidraco) in China, causes almost 100% mortality in yellow catfish [9,12].…”

“…Genome sequence of strain SCCF01 was the only complete genome sequence of V. mimicus from infected aquatic animals. Previous studies have shown that V. mimicus strain SCCF01 natural infection can cause high mortality rate in fishes [9], and is also a highly virulent in the artificial infection experiment [12,13]. However, the genetic features and evolutionary strategies of V. mimicus from fish remain unknown.…”

Strategies for Prevention and Control of Vibriosis in Asian Fish Culture

“…Identifications of several possible virulence genes have been made in order to determine the complete genome sequence of V. mimicus strain sccf01 related to yellow catfish infection, which contributed to the development of subunit and lives attenuated vaccines by providing a genetic basis. Moreover, the immersion challenge test depicted that strain SCCF01 can adhere and colonize the mucosal surface, which is hopeful for the development of an attenuated vaccine [ 89 ].…”

The cAMP Receptor Protein (CRP) of Vibrio mimicus Regulates Its Bacterial Growth, Type II Secretion System, Flagellum Formation, Adhesion Genes, and Virulence

“…In addition to its well-established role in regulating virulence factors, the Crp-cAMP complex has been implicated in regulating various activities, including motility ability [17], biofilm formation [18], host colonization [19], pheromone signaling [20], natural competence [21], osmotolerance [22], lipopolysaccharide modifications [23], resistance to bacteriophages [24], and integrase activity [25]. The crp gene has been identified in whole genome sequencing of SCCF01 by our laboratory [26]. Nevertheless, the current knowledge of V. mimicus is still limited.…”