Complete Genome Sequence of Lactococcus lactis IO-1, a Lactic Acid Bacterium That Utilizes Xylose and Produces High Levels of
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            -Lactic Acid

Katô, Hiroaki; Shiwa, Yuh; Oshima, Koichiro; Machii, Miki; Araya-Kojima, Tomoko; Zendo, Takeshi; Shimizu-Kadota, Mariko; Hattori, Masahira; Sonomoto, Kenji; Yoshikawa, Hideki

doi:10.1128/jb.00074-12

Cited by 53 publications

(32 citation statements)

References 13 publications

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“…Currently eight complete genome sequences of L. lactis are known [1,3,4,12,19,32,38,50]. Blast searches using the LysM domain sequences of the major autolysin AcmA [7] showed that each strain putatively expresses five proteins containing one or more LysM repeats at their C-terminus, except L.…”

Section: Introductionmentioning

confidence: 99%

AcmD, a Homolog of the Major Autolysin AcmA of Lactococcus lactis, Binds to the Cell Wall and Contributes to Cell Separation and Autolysis

Visweswaran

Steen

Leenhouts³

et al. 2013

PLoS ONE

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Lactococcus lactis expresses the homologous glucosaminidases AcmB, AcmC, AcmA and AcmD. The latter two have three C-terminal LysM repeats for peptidoglycan binding. AcmD has much shorter intervening sequences separating the LysM repeats and a lower iso-electric point (4.3) than AcmA (10.3). Under standard laboratory conditions AcmD was mainly secreted into the culture supernatant. An L. lactis acmAacmD double mutant formed longer chains than the acmA single mutant, indicating that AcmD contributes to cell separation. This phenotype could be complemented by plasmid-encoded expression of AcmD in the double mutant. No clear difference in cellular lysis and protein secretion was observed between both mutants. Nevertheless, overexpression of AcmD resulted in increased autolysis when AcmA was present (as in the wild type strain) or when AcmA was added to the culture medium of an AcmA-minus strain. Possibly, AcmD is mainly active within the cell wall, at places where proper conditions are present for its binding and catalytic activity. Various fusion proteins carrying either the three LysM repeats of AcmA or AcmD were used to study and compare their cell wall binding characteristics. Whereas binding of the LysM domain of AcmA took place at pHs ranging from 4 to 8, LysM domain of AcmD seems to bind strongest at pH 4.

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Section: Introductionmentioning

confidence: 99%

AcmD, a Homolog of the Major Autolysin AcmA of Lactococcus lactis, Binds to the Cell Wall and Contributes to Cell Separation and Autolysis

Visweswaran

Steen

Leenhouts³

et al. 2013

PLoS ONE

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“…The numbers of the ORFs were revised after a minute investigation by us of the genome deposited in DDBJ/EMBL/GenBank. 9) Similarly to L. lactis IL1403, 13) the GC skew has two shift points on the circular genome map of strain IO-1 ( Fig. 1), indicating that these points are the loci of ori and ter.…”

Section: General Genome Featuresmentioning

confidence: 99%

“…AP012281. 9) The genes related to the PP/glycolytic and PK pathways (except for the gene for transaldolase), and those involved in L-lactate fermentation, were found in the genome. Here, detailed analyses of its genomic traits in relation to prophages, nisin-sucrose transposon, restriction-modification systems, and the synthetic pathways of amino acids and vitamins were done.…”

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confidence: 99%

Genomic Features ofLactococcus lactisIO-1, a Lactic Acid Bacterium That Utilizes Xylose and Produces High Levels ofL-Lactic Acid

Shimizu-Kadota

Katô

Shiwa

et al. 2013

Bioscience, Biotechnology, and Biochemistry

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Lactococcus lactis IO-1 (JCM7638) produces L-lactic acid predominantly when grown at high xylose concentrations, and its utilization is highly desired in the green plastics industry. Therefore it is worthwhile studying its genomic traits. In this study, we focused on (i) genes of possible horizontal transfer derivation (prophages, the nisin-sucrose transposon, and several restrictionmodification systems), and (ii) genes for the synthetic pathways of amino acids and vitamins in the IO-1 genome. In view of the results of this analysis, we consider their meanings in strain IO-1.

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“…Subsequent sequencing showed that Lc. lactis IO-1 contains transketolase but no transaldolase, and thus is thought to use 6-phosphofructokinase and fructose bisphosphate aldolase as alternate enzymes to complete the higher yielding PP pathway (Kato et al 2012). Furthermore, the strain exclusively produces L-lactic acid, as genes coding D-lactate dehydrogenase (D-LDH) and lactate racemase are absent (Shimizu-Kadota et al 2013).…”

Section: Conversion Of Pentoses To Lactic Acidmentioning

confidence: 98%

Microbial production of lactic acid

Eiteman

Ramalingam

2015

Biotechnol Lett

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Lactic acid is an important commodity chemical having a wide range of applications. Microbial production effectively competes with chemical synthesis methods because biochemical synthesis permits the generation of either one of the two enantiomers with high optical purity at high yield and titer, a result which is particularly beneficial for the production of poly(lactic acid) polymers having specific properties. The commercial viability of microbial lactic acid production relies on utilization of inexpensive carbon substrates derived from agricultural or waste resources. Therefore, optimal lactic acid formation requires an understanding and engineering of both the competing pathways involved in carbohydrate metabolism, as well as pathways leading to potential by-products which both affect product yield. Recent research leverages those biochemical pathways, while researchers also continue to seek strains with improved tolerance and ability to perform under desirable industrial conditions, for example, of pH and temperature.

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Complete Genome Sequence of Lactococcus lactis IO-1, a Lactic Acid Bacterium That Utilizes Xylose and Produces High Levels of l -Lactic Acid

Cited by 53 publications

References 13 publications

AcmD, a Homolog of the Major Autolysin AcmA of Lactococcus lactis, Binds to the Cell Wall and Contributes to Cell Separation and Autolysis

AcmD, a Homolog of the Major Autolysin AcmA of Lactococcus lactis, Binds to the Cell Wall and Contributes to Cell Separation and Autolysis

Genomic Features ofLactococcus lactisIO-1, a Lactic Acid Bacterium That Utilizes Xylose and Produces High Levels ofL-Lactic Acid

Microbial production of lactic acid

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