Complete genome sequence of Mycobacterium tuberculosis K from a Korean high school outbreak, belonging to the Beijing family

Abstract

Mycobacterium tuberculosis K, a member of the Beijing family, was first identified in 1999 as the most prevalent genotype in South Korea among clinical isolates of M. tuberculosis from high school outbreaks. M. tuberculosis K is an aerobic, non-motile, Gram-positive, and non-spore-forming rod-shaped bacillus. A transmission electron microscopy analysis displayed an abundance of lipid bodies in the cytosol. The genome of the M. tuberculosis K strain was sequenced using two independent sequencing methods (Sanger and Illumina). Here, we present the genomic features of the 4,385,518-bp-long complete genome sequence of M. tuberculosis K (one chromosome, no plasmid, and 65.59 % G + C content) and its annotation, which consists of 4194 genes (3447 genes with predicted functions), 48 RNA genes (3 rRNA and 45 tRNA) and 261 genes with peptide signals.Electronic supplementary materialThe online version of this article (doi:10.1186/s40793-015-0071-4) contains supplementary material, which is available to authorized users.

Protective Vaccine Efficacy of the Complete Form of PPE39 Protein from Mycobacterium tuberculosis Beijing/K Strain in Mice

“…Unlike other PE-PPE proteins located in a row in the ESX region (20), MTBK_24820 exists independently, without PE proteins in the insertion region. MTBK_24820 is a PPE-MTPR subfamily with repeats of NxGxGNxG in the C terminus (17,21) and is orthologous to the M. tuberculosis H37Rv PPE39 protein (annotated Rv2353c) (22). PPE39 has a number of highly genetic variables among several M. tuberculosis isolates, caused by IS6110 integration and the addition of single-nucleotide polymorphisms (SNPs) (23).…”

“…AIB49026.1) within the 5.7-kb insertion region compared with the genome of H37Rv strain (17). In the inserted region, ESAT-6 like (esx) proteins, such as InsB (MTBK_24790) and InsC (MTBK_24800) (18,19), and PPE proteins, including InsD (MTBK_24810; partial form of PPE38) and MTBK_24820 (N-terminally added PPE39 protein), are inserted in a row.…”

Pemanfaatan bioinformatika dalam bidang pertanian dan kesehatan (The utilization of bioinformatics in the field of agriculture and health)

“…Salah satu contohnya adalah keberadaan basis data FIND Tuberculosis Strain Bank yang membantu peneliti dalam melakukan uji patogenisitas dan desain obat untuk mengatasi penyakit tuberculosis (TBC) (Tessema et al, 2017). Disisi lain, penelitian terkait penyakit tersebut juga maju pesat dikarenakan keberadaan basis data NGS yang membantu dalam anotasi genom patogen penyebab penyakitnya yaitu Mycobacterium tuberculosis (Skvortsov et al, 2013;Han et al, 2015;Mokrousov et al, 2016). Keberadaan basis data tersebut sebagian besar digunakan untuk desain obat secara in silico dengan memanfaatkan kemudahan sistem daring.…”

Identification of Mycobacterium tuberculosis isolated from culture-negative pulmonary and extra-pulmonary samples in cases of suspected tuberculosis

“…The genome of M. tuberculosis is about 4,300 kb [4], [5]. For example, the genome of M. tuberculosis that was sequenced by the Sanger and Illumina methods is 4,385 kb long [4].…”

“…The genome of M. tuberculosis is about 4,300 kb [4], [5]. For example, the genome of M. tuberculosis that was sequenced by the Sanger and Illumina methods is 4,385 kb long [4]. The complete genome length of M. tuberculosis , CCDC 5079 and CCDC 5080 strains, is 4,399 kb and 4,406 kb, respectively [5].…”