Complete Genome Sequence of Seneca Valley Virus CH-01-2015 Identified in China

Wu, Qiwen; Zhao, Xiujuan; Chen, Yanshan; He, Xiaoming; Zhang, Guanqun; Ma, Jingyun

doi:10.1128/genomea.01509-15

Cited by 58 publications

(43 citation statements)

References 5 publications

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“…In 2015, SVA was first reported by Wu Q et al in China [15]. Thereafter, an increased number of cases of SVA have been reported in China, including Guangdong, Hubei, Guangxi, Fujian, Henan, Anhui, and Heilongjiang [6,[16][17][18][19][20]. SVA can cause idiopathic vesicular disease in pigs.…”

Section: Discussionmentioning

confidence: 99%

Comparison of the Pathogenicity of Two Different Branches of Senecavirus a Strain in China

Zhang

Hao

et al. 2020

Pathogens

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Senecavirus A (SVA), an emerging infectious disease, is associated with the porcine idiopathic vesicular disease. Here, the pathogenesis of different strains of SVA was investigated in growing-finishing pigs. We aimed to evaluate the replication characteristics, virus particle morphology, clinical signs, and vesicular lesions in comparison with two different strains of SVA. The animals were infected with SVA HB-CH-2016 or CH/AH-02/2017 by intranasal routes (3 mL, 109TCID50/mL) and monitored daily for 14 days post-inoculation (dpi) for clinical signs and vesicular lesions. Viremia or viral shedding was detected in the blood, fecal swab, and nasal swab samples. Results showed no distinct differences in plaque size, replication ability, and characteristic virions between SVA HB-CH-2016 and CH/AH-02/2017 strains. Animal experimental results showed that both SVA CH/AH-02/2017 and SVA HB-CH-2016 could infect pigs. However, an obvious difference in the pathogenicity and dynamics of infection was observed between SVA HB-CH-2016 and CH/AH-02/2017 strains. The pathogenesis of SVA CH/AH-02/2017 was similar to that of published results of USA strains, whereas the SVA HB-CH-2016 strain had low pathogenicity to pigs. Clinical signs and vesicular lesions were observed in SVA CH/AH-02/2017-infected pigs. Additionally, the different branches of SVA should be capable of inducing broad cross-reactive neutralizing antibodies, which play an important role in clearing the SVA virus. This study of animal models for SVA infection will be beneficial to develop vaccines and antivirals.

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Section: Discussionmentioning

confidence: 99%

Comparison of the Pathogenicity of Two Different Branches of Senecavirus a Strain in China

Zhang

Hao

et al. 2020

Pathogens

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“…The SVV‐1 is a single‐stranded positive‐sense RNA virus, belonging to the genus Senecavirus in the family Picornaviridae . Since its discovery in 1998, SVV‐1 has been reported in swine population globally, including the United States, Canada, Brazil, Colombia, Thailand and China (Armson et al, ; Fowler et al, ; Leme et al, ; Pasma, Davidson, & Shaw, ; Saeng‐chuto et al, ; Sun, Vannucci, Knutson, Corzo, & Marthaler, ; Wu et al, ). The SVV‐1 causes vesicular disease in pigs, which is clinically indistinguishable from FMD and characterized by vesicles on the snouts and coronary bands, lameness, anorexia, lethargy, fever and diarrhoea (Hause et al, ; Segalés et al, ).…”

Section: Introductionmentioning

confidence: 99%

Development and evaluation of multiplex real‐time RT‐PCR assays for the detection and differentiation of foot‐and‐mouth disease virus and Seneca Valley virus 1

Wang

Das

Zheng

et al. 2019

Transbound Emerg Dis

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Foot‐and‐mouth disease virus (FMDV) causes a highly contagious and economically important vesicular disease in cloven‐hoofed animals that is clinically indistinguishable from symptoms caused by Seneca Valley virus 1 (SVV‐1). To differentiate SVV‐1 from FMDV infections, we developed a SVV‐1 real‐time RT‐PCR (RT‐qPCR) assay and multiplexed with published FMDV assays. Two published FMDV assays (Journal of the American Veterinary Medical Association, 220, 2002, 1636; Journal of Virological Methods, 236, 2016, 258) targeting the 3D polymerase (3D) region were selected and multiplexed with the SVV‐1 assay that has two targets, one in the 5′ untranslated region (5′ UTR, this study) and the other in the 3D region (Journal of Virological Methods, 239, 2017, 34). In silico analysis showed that the primers and probes of SVV‐1 assay matched 98.3% of the strain sequences (113/115). The primer and probe sequences of the Shi FMDV assay matched 85.4% (806/944), and that of the Callahan FMDV assay matched 62.7% (592/944) of the sequences. The limit of detection (LOD) for the two multiplex RT‐qPCR assays for SVV‐1 was both 9 copies per reaction by cloned positive plasmids and 0.16 TCID50 per reaction by cell culture. The LOD for FMDV by both multiplex assays was 11 copies per reaction using cloned positive plasmids. With cell cultures of the seven serotypes of FMDV, the Shi assay (Journal of Virological Methods, 236, 2016, 258) had LODs between 0.04 and 0.18 TCID50 per reaction that were either the same or lower than the Callahan assay. Interestingly, multiplexing with SVV‐1 increased the amplification efficiencies of the Callahan assay (Journal of the American Veterinary Medical Association, 220, 2002, 1636) from 51.5%–66.7% to 89.5%–96.6%. Both assays specifically detected the target viruses without cross‐reacting to SVV‐1 or to other common porcine viruses. An 18S rRNA housekeeping gene that was amplified from multiple cloven‐hoofed animal species was used as an internal control. The prevalence study did not detect any FMDV, but SVV‐1 was detected from multiple types of swine samples with an overall positive rate of 10.5% for non‐serum samples.

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“…The virus was isolated incidentally in PER.C6 cell cultures in 2002 (Hales et al., ) and was subsequently discovered in pigs imported to the United States from Canada in 2007 (Pasma et al., ). The complete genome sequence of SVV‐001 was described in 2005 (Knowles and Hallenbeck, ), and more SVA genomes have been sequenced in recent years (Vannucci et al., ; Zhang et al., ; Wu et al., ). SVV‐001 is thought to have potent oncolytic activity against tumours with neuroendocrine features while it is not harmful to normal human cells (Reddy et al., ), making it a promising candidate for oncolytic virus therapy.…”

Section: Introductionmentioning

confidence: 99%

The First Identification and Complete Genome of Senecavirus A Affecting Pig with Idiopathic Vesicular Disease in China

Zhao

Bai

et al. 2016

Transbound Emerg Dis

146

113

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Senecavirus A (SVA) infection was recently confirmed in pigs in Brazil. In March, 2015, an outbreak of vesicular disease occurred in Guangdong, China, characterized by vesicular lesions in sows and acute death of neonatal piglets. Cumulative incidence of porcine idiopathic vesicular disease in farm A was 258, which had a total number of 5500 sows. Sows in farm B displayed typical vesicular symptoms by May, 2015, which also had 5500 sows. A total of 278 and 142 of 5500 sows in farm B demonstrated lame and presented vesicles, respectively, associated with a total of 186 mortality in piglets. Routine differential diagnoses for swine vesicular disease were carried out to exclude infection with foot-and-mouth disease virus, swine vesicular disease virus, vesicular exanthema of swine virus and vesicular stomatitis virus. In this study, seven pairs of primer were designed to amplify the complete genome of SVA in RT-PCR assays. Sequence alignment showed that this Chinese strain shares 94.4-97.1% sequence identity to other eight strains of SVA. This is the first report of SVA in China and provides information about the association between SVA infection and vesicular disease.

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Complete Genome Sequence of Seneca Valley Virus CH-01-2015 Identified in China

Cited by 58 publications

References 5 publications

Comparison of the Pathogenicity of Two Different Branches of Senecavirus a Strain in China

Comparison of the Pathogenicity of Two Different Branches of Senecavirus a Strain in China

Development and evaluation of multiplex real‐time RT‐PCR assays for the detection and differentiation of foot‐and‐mouth disease virus and Seneca Valley virus 1

The First Identification and Complete Genome of Senecavirus A Affecting Pig with Idiopathic Vesicular Disease in China

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