Complete Genome Sequence of the Nitrogen-Fixing and Rhizosphere-Associated Bacterium Pseudomonas stutzeri Strain DSM4166

Abstract

We present here the analysis of the whole-genome sequence of Pseudomonas stutzeri strain DSM4166, a diazotrophic isolate from the rhizosphere of a Sorghum nutans cultivar. To our knowledge, this is the second genome to be sequenced for P. stutzeri. The availability and analysis of the genome provide insight into the evolution of the nitrogen fixation property and identification of rhizosphere competence traits required in interactions with host plants.

Genome Sequences of Pseudomonas luteola XLDN4-9 and Pseudomonas stutzeri XLDN-R, Two Efficient Carbazole-Degrading Strains

“…The largest contigs of strains XLDN4-9 and XLDN-R are 265,571 and 235,018 bp. The draft genome sequence of strain XLDN4-9 consists of 4,627,073 bases with a GϩC content of 54.2%, while the draft genome sequence of strain XLDN-R contains 4,695,416 bases with a GϩC content of 63.9%, which is in accordance with other P. stutzeri strains (6,14,15). There were 45 and 58 predicted tRNAs, 4,454 and 4,247 coding sequences, and 444 and 496 subsystems in strains XLDN4-9 and XLDN-R, respectively.…”

Comparative genomic, proteomic and exoproteomic analyses of three Pseudomonas strains reveals novel insights into the phosphorus scavenging capabilities of soil bacteria

“…Various Pseudomonas strains can also degrade organic P compounds, such as phytate, phosphonates and phosphites (Ternan and Quinn, 1998; White and Metcalf, 2004, 2007). Three Pseudomonas strains , Pseudomonas putida BIRD‐1, Pseudomonas fluorescens SBW25 and Pseudomonas stutzeri DSM4166 (hereafter, BIRD‐1, SBW25 and DSM4166 respectively) are three examples of PGPR (Naseby et al ., 2001; Hass and Keel, 2003; Preston, 2004; Yu et al ., 2011; Roca et al ., 2013). SBW25 inhabits the rhizosphere of Pea plants and is antagonistic towards the pathogen Pythium ultimum (Naseby et al ., 2001), whereas DSM4166, an ‘unusual’ nitrogen‐fixing bacterium, was isolated from a cultivar of Sorghum nutans (Yu et al ., 2011).…”

“…Three Pseudomonas strains , Pseudomonas putida BIRD‐1, Pseudomonas fluorescens SBW25 and Pseudomonas stutzeri DSM4166 (hereafter, BIRD‐1, SBW25 and DSM4166 respectively) are three examples of PGPR (Naseby et al ., 2001; Hass and Keel, 2003; Preston, 2004; Yu et al ., 2011; Roca et al ., 2013). SBW25 inhabits the rhizosphere of Pea plants and is antagonistic towards the pathogen Pythium ultimum (Naseby et al ., 2001), whereas DSM4166, an ‘unusual’ nitrogen‐fixing bacterium, was isolated from a cultivar of Sorghum nutans (Yu et al ., 2011). BIRD‐1, a P‐solubilising bacterium, has been previously utilized as a bioinoculant, since it can significantly improve the germination rates, growth and yields of various agricultural crops (Roca et al ., 2013).…”

Draft Genome of Pseudomonas stutzeri Strain ZoBell (CCUG 16156), a Marine Isolate and Model Organism for Denitrification Studies

“…So far, whole-genome sequences of 3 P. stutzeri strains of genomovar 1 are publicly available: the type strain of the species (of clinical origin) (CGMCC 1.1803 ϭ ATCC 17588) (2) and two dinitrogen fixers isolated from the rhizosphere (A1501 and CMT.9.A) (8,9). Therefore, analysis of the marine strain ZoBell, the first sequenced strain representative of genomovar 2, will help us gain insight into the evolution of the species by analyzing members of a different genomovar and studying the adaptation of P. stutzeri strains to marine habitats.…”