Complete Genome Sequence of the Anaerobic, Protein-Degrading Hyperthermophilic Crenarchaeon
            <i>Desulfurococcus kamchatkensis</i>

Ravin, Nikolai V.; Mardanov, Andrey V.; Beletsky, Alexey V.; Kublanov, Ilya V.; Kolganova, T. V.; Lebedinsky, Alexander V.; Chernyh, N. A.; Bonch-Osmolovskaya, E. A.; Skryabin, K. G.

doi:10.1128/jb.01525-08

Cited by 37 publications

(31 citation statements)

References 55 publications

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“…3). These data are in line with the presence of the biotindependent carboxylases in all Sulfolobales studied so far (Norris et al, 1989;Burton et al, 1999;Ishii et al, 1996;Chuakrut et al, 2003;Hügler et al, 2003a, b;Menendez et al, 1999) as well as with the absence of these proteins and the corresponding genes in the sequenced Desulfurococcales genomes (Hügler et al, 2003a;Jahn et al, 2007;Anderson et al, 2009;Brügger et al, 2007;Kawarabayasi et al, 1999;Podar et al, 2008;Ravin et al, 2009).…”

Section: Detection Of Biotin-containing Proteins In Cell Extractssupporting

confidence: 58%

“…It can grow aerobically or anaerobically by nitrate respiration (Blöchl et al, 1997). The sequence and the annotation of its genome are not available yet, and it is not known if this species contains the genes encoding biotin-dependent carboxylases, though the sequenced genomes of members of the Desulfurococcales lack them (Anderson et al, 2009;Brügger et al, 2007;Kawarabayasi et al, 1999;Podar et al, 2008;Ravin et al, 2009). Moreover, autotrophic pathways functioning in Pyrodictiaceae are completely unknown.…”

Section: Introductionmentioning

confidence: 99%

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Study of the distribution of autotrophic CO2 fixation cycles in Crenarchaeota

et al. 2010

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Two new autotrophic carbon fixation cycles have been recently described in Crenarchaeota. The 3-hydroxypropionate/4-hydroxybutyrate cycle using acetyl-coenzyme A (CoA)/propionyl-CoA carboxylase as the carboxylating enzyme has been identified for (micro)aerobic members of the Sulfolobales. The dicarboxylate/4-hydroxybutyrate cycle using oxygen-sensitive pyruvate synthase and phosphoenolpyruvate carboxylase as carboxylating enzymes has been found in members of the anaerobic Desulfurococcales and Thermoproteales. However, Sulfolobales include anaerobic and Desulfurococcales aerobic autotrophic representatives, raising the question of which of the two cycles they use. We studied the mechanisms of autotrophic CO 2 fixation in the strictly anaerobic Stygiolobus azoricus (Sulfolobales) and in the facultatively aerobic Pyrolobus fumarii (Desulfurococcales). The activities of all enzymes of the 3-hydroxypropionate/4-hydroxybutyrate cycle were found in the anaerobic S. azoricus. In contrast, the aerobic or denitrifying P. fumarii possesses all enzyme activities of the dicarboxylate/4-hydroxybutyrate cycle. We conclude that autotrophic Crenarchaeota use one of the two cycles, and that their distribution correlates with the 16S rRNA-based phylogeny of this group, rather than with the aerobic or anaerobic lifestyle.

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Section: Detection Of Biotin-containing Proteins In Cell Extractssupporting

confidence: 58%

Section: Introductionmentioning

confidence: 99%

Study of the distribution of autotrophic CO2 fixation cycles in Crenarchaeota

et al. 2010

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“…In addition, differences in 16S rRNA genes from those of D. mobilis (Kjems et al, 1987) and D. amylolyticus (Tourova et al, 2000) were then used for the proposal of D. fermentans (Perevalova et al, 2005) and D. kamchatkensis (Kublanov et al, 2009). However, later it became clear that the quality of some of these sequences was quite low, and the results of their comparison should be treated with caution.…”

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confidence: 99%

“…The latter capacity was even more prominent in another Kamchatka isolate, Desulfurococcus fermentans, able to grow on several sugars and polysaccharides including cellulosic substrates (Perevalova et al, 2005). The most recently described Kamchatka isolate, Desulfurococcus kamchatkensis, differed phenotypically from the other isolates by its ability to utilize non-hydrolysed structural proteins (alpha-keratin) (Kublanov et al, 2009).…”

mentioning

confidence: 99%

“…The first sequenced complete genome of a Desulfurococcus representative was that of D. kamchatkensis (Ravin et al, 2009), followed by the complete genomes of D. mucosus (Wirth et al, 2011) and D. fermentans (Susanti et al, 2012) For the in silico hybridization, we used the genome sequences of the type strains of Desulfurococcus species from NCBI GenBank. In silico prediction of DDH values was performed using GGDC 2.0 BLAST+ at the DSMZ GGDC site (MeierKolthoff et al, 2013) and produced results that correlated with the 16S rRNA gene sequence similarity values.…”

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confidence: 99%

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Reclassification of Desulfurococcus mobilis as a synonym of Desulfurococcus mucosus, Desulfurococcus fermentans and Desulfurococcus kamchatkensis as synonyms of Desulfurococcus amylolyticus, and emendation of the D. mucosus and D. amylolyticus species descriptions

Perevalova¹,

Kublanov²,

Bidzhieva³

et al. 2016

International Journal of Systematic and Evolutionary Microbiology

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Reclassification ofRepresentatives of the genus Desulfurococcus were among the first hyperthermophilic archaea to be isolated and described (Zillig et al., 1982;Zillig & Stetter, 1983). All Desulfurococcus species are obligate anaerobes with coccoid cells; they belong to the family Desulfurococcaceae and the order Desulfurococcales in the archaeal phylum Crenarchaeota. The first two species described, Desulfurococcus mucosus and Desulfurococcus mobilis, were isolated from Icelandic terrestrial hot springs; they were characterized as organotrophs, capable of utilizing a wide range of proteinaceous substrates by fermentation, facultatively coupled with the reduction of elemental sulfur to hydrogen sulfide (Zillig et al., 1982). Other members of the genus were Abbreviations: ANI, average nucleotide identity; DDH, DNA-DNA hybridization.

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Fervidicoccus

Perevalova¹,

Lebedinsky²,

Bonch-Osmolovskaya³

2016

Bergey's Manual of Systematics of Archaea and Bacteria

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Fer.vi.do.coc'cus L. adj. fervidus hot; L. masc. n. coccus a berry and, in biology, a coccoid cell; N.L. masc. n. Fervidicoccus coccus that grows at high temperatures. Crenarchaeota / Thermoprotei / Fervidicoccales / Fervidicoccaceae / Fervidicoccus Members of the genus Fervidicoccus are extremely thermophilic, obligately anaerobic, and organotrophic microorganisms of the phylum Crenarchaeota . 16S rRNA genes of closely related microorganisms were found in environmental DNA samples from terrestrial hot springs of Kamchatka and Kuril Islands (Russia), Iceland, Yellowstone National Park (USA), and New Zealand with temperatures of 55–84°C, where they can make up a significant fraction of the microbial community. At present, the only cultivated representative of the Fervidicoccus genus is Fervidicoccus fontis, isolated from the Treshchinnyi hot spring of Uzon Caldera, Kamchatka. It is an anaerobic organotroph growing optimally on peptides in the presence of 20–50 mg/l yeast extract at 65–70°C and pH 6.0. Elemental sulfur, if present, is reduced to H 2 S; however, it is not obligately required for growth. H 2 partially inhibits the growth of Fervidicoccus fontis . Thus, phenotypically Fervidicoccus fontis resembles other anaerobic organotrophic archaea of the phyla Crenarchaeota and Euryarchaeota , such as desulfurococci and thermococci, but differs from them by its lower growth temperature. Complete genome analysis of Fervidicoccus fontis confirmed a distinct phylogenetic position of this group. DNA G + C content (mol%) : 37.5 (genome). Type species : Fervidicoccus fontis Perevalova, Bidzhieva, Kublanov, Hinrichs, Liu, Kolganova, Lebedinsky and Bonch‐Osmolovskaya 2010, 2086.

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Complete Genome Sequence of the Anaerobic, Protein-Degrading Hyperthermophilic Crenarchaeon Desulfurococcus kamchatkensis

Cited by 37 publications

References 55 publications

Study of the distribution of autotrophic CO2 fixation cycles in Crenarchaeota

Study of the distribution of autotrophic CO2 fixation cycles in Crenarchaeota

Reclassification of Desulfurococcus mobilis as a synonym of Desulfurococcus mucosus, Desulfurococcus fermentans and Desulfurococcus kamchatkensis as synonyms of Desulfurococcus amylolyticus, and emendation of the D. mucosus and D. amylolyticus species descriptions

Fervidicoccus

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