Reclassification of Desulfurococcus mobilis as a synonym of Desulfurococcus mucosus, Desulfurococcus fermentans and Desulfurococcus kamchatkensis as synonyms of Desulfurococcus amylolyticus, and emendation of the D. mucosus and D. amylolyticus species descriptions

Abstract

Reclassification ofRepresentatives of the genus Desulfurococcus were among the first hyperthermophilic archaea to be isolated and described (Zillig et al., 1982;Zillig & Stetter, 1983). All Desulfurococcus species are obligate anaerobes with coccoid cells; they belong to the family Desulfurococcaceae and the order Desulfurococcales in the archaeal phylum Crenarchaeota. The first two species described, Desulfurococcus mucosus and Desulfurococcus mobilis, were isolated from Icelandic terrestrial hot springs; they were characterized as organotrophs, capable of utilizing a wide range of proteinaceous substrates by fermentation, facultatively coupled with the reduction of elemental sulfur to hydrogen sulfide (Zillig et al., 1982). Other members of the genus were Abbreviations: ANI, average nucleotide identity; DDH, DNA-DNA hybridization.

Metabolic reconstruction and experimental verification of glucose utilization in Desulfurococcus amylolyticus DSM 16532

“…D. amylolyticus DSM 16532 exhibited the highest gene similarity to D. amylolyticus 1221n (formerly known as Desulfuroccus kamchatkensis ) and to D. amylolyticus Z-533 T (Supplementary Table 1 ) . This finding is not very surprising as D. amylolyticus DSM 16532 and D. amylolyticus 1221n were reclassified as synonyms of D. amyloyticus (Perevalova et al 2016 ). According to our BLAST analyses, the carbon substrates could be channeled to the central catabolic pathway by 97 ABC transporter family genes, e.g., Desfe_0184, Desfe_0187, Desfe_0620, Desfe_0639, Desfe_0721, and Desfe_0754 as summarized in Supplementary Tables 1 and 2 .…”

“…D. amylolyticus DSM 16532 (Z-1312), formerly known as D. fermentans (Perevalova et al 2016 ; Perevalova et al 2005 ), was purchased from the Deutsche Sammlung von Mikroorganismen und Zellkulturen (DSMZ). A modified DSMZ medium No.…”

“…Among the Desulfurococcales, Desulfurococcus amylolyticus DSM 16532, formerly known as Desulfurococcus fermentans (Perevalova et al 2016 ; Perevalova et al 2005 ), was characterized as an anaerobic, hyperthermophilic crenarchaeon. D. amylolyticus was isolated from a freshwater hot spring in the Uzon caldera (Kamchatka Penninsula, Russian Federation).…”

Unraveling the multiplicity of geranylgeranyl reductases in Archaea: potential roles in saturation of terpenoids

“…Organisms belonging to Desulfurococcales are hyperthermophiles which are obligate anaerobes and organoheterotrophs (Perevalova et al 2005 ). Desulfurococcus amylolyticus (formerly known as D. fermentans ) is a representative organism from this order which is able to metabolize formate (Ergal et al 2020 ; Perevalova et al 2016 ).…”

Permanent Draft Genome Sequence of Desulfurococcus amylolyticus Strain Z-533 T , a Peptide and Starch Degrader Isolated from Thermal Springs in the Kamchatka Peninsula and Kunashir Island, Russia

“…The 16S rRNA gene sequences of D. amylolyticus Z-533 T differ by 0.1 to 0.3% from those of other D. amylolyticus strains, namely, Z-1312 and 1221n (2–4), which were formerly known as D. fermentans Z-1312 and D. kamchatkensis 1221n, respectively (5). Of these, only D. amylolyticus Z-1312 degrades cellulose (2) and is not inhibited by the presence of hydrogen (6).…”