Complete Genome Sequence of the Blochmannia Endosymbiont of Camponotus nipponensis

Abstract

Blochmannia endosymbionts, belonging to Gammaproteobacteria, live in bacteriocytes, which are specialized cells for these bacterial species in the Camponotus genus (carpenter ants). In this announcement, we describe the complete genome sequence of the Blochmannia endosymbiont of Camponotus nipponensis, which originated from a C. nipponensis colony collected in the Republic of Korea.

Complete Genome Sequence of a Blochmannia Endosymbiont of Colobopsis nipponica

“…Their roles have been predicted to enrich the nourishment and immunity of the host ants in the bacteriocytes of the ants’ midgut ( 2 , 4 , 5 ). Six Blochmannia complete genomes are available ( 6 – 9 ), which can be used to understand species-specific adaptations at the genomic level.…”

“…A total of 609 protein-coding genes (PCGs), 40 tRNAs, 3 rRNAs, 2 noncoding RNAs, and 1 transfer-messenger RNA were predicted. The number of PCGs is the third highest among all available Blochmannia complete genomes, after those of the Blochmannia endosymbionts of Camponotus nipponensis ( 9 ) and Camponotus pennsylvanicus ( 6 ). The number of tRNAs is the same as those of the aforementioned two genomes, the highest among seven genomes.…”

The complete mitochondrial genome of new species candidate of Rosa rugosa (Rosaceae)

“…Genome was sequenced using HiSeqX at Macrogen Inc., Korea, and de novo assembly and gap-filling process were performed by Velvet v1.2.10 (Zerbino and Birney 2008) and SOAPGapCloser v1.12 (Zhao et al 2011), respectively, and confirmation of each assembled bases and correctly assembled sequences done by BWA v0.7.17 (Li 2013), and SAMtools v1.9 (Li et al 2009) under the environment of Genome Information System (GeIS; http://geis.infoboss.co.kr/; Park et al in preparation). In addition, circular test was conducted for confirming that our mitochondrial genome is circular form using SOAPGapCloser v1.12 in the same way used in completing bacterial genome (Park et al 2020). Mitochondrial genome annotation was conducted with Mitofy (Alverson et al 2010) and then annotated genes were confirmed by comparing with mitochondrial genome of Rosa chinensis (PDCK01000047) under the environment of Geneious R11 11.0.5 (Biomatters Ltd., Auckland, New Zealand).…”

“…In addition, circular test was conducted for confirming that our mitochondrial genome is circular form using SOAPGapCloser v1.12 in the same way used in completing bacterial genome (Park et al. 2020 ). Mitochondrial genome annotation was conducted with Mitofy (Alverson et al.…”

The complete mitochondrial genome of Aphis gossypii Glover, 1877 (Hemiptera: Aphididae) isolated from Leonurus japonicus in Korea

“… 2020 ; Park, Xi, Park, Lee 2020 ; Park, Xi, Park, Nam, et al. 2020 ; Oh et al. in preparation ), we sequenced and prepared the genomes from the L. japonicus sample with A. gossypii DNA (37.690627 N, 128.594893E; InfoBoss Cyber Herbarium (IN); INH-00026) extracted using DNeasy Plant Mini Kit (QIAGEN, Hilden, Germany).…”