Complete Genome Sequence of Vaccinia Virus Strain L-IVP

Shvalov, Аlexander N.; Сиволобова, Г. Ф.; Kuligina, Elena V.; Кочнева, Г. В.

doi:10.1128/genomea.00372-16

Cited by 7 publications

(4 citation statements)

References 6 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Сообщалось, что штамм Lister кодирует больше генов, участвующих в уклонении от иммунного ответа, таких как A53R, рецептор растворимого фактора некроза опухоли, или T1/35 кДаингибитор CC-хемокинов, которые отсутствуют в других штаммах, таких как MVA или WR (Western Reserve) [9,10]. LIVP является биовариантом английского штамма Lister, полученным путем его адаптации в коже теленка [11]. Этот штамм частично использовали в программе ликвидации оспы после 1971 г., и, как сообщается, он обладает онколитическими свойствами и значительно меньшей вирулентностью по сравнению с другими биовариантами штамма Lister [12,13], кроме этого, он не был изучен в ряде доклинических и клинических исследований [14][15][16][17][18][19].…”

Section: Bulletin Of Rsmu 2 2023 Vestnikrgmuru | |unclassified

Sravnenie onkoliticheskoj aktivnosti rekombinantnyh shtammov virusa ospovakciny LIVP-RFP i MVA-RFP v otnoshenii solidnyh opuholej

Шакиба¹,

Набережная²,

Кочетков³

et al. 2023

Вестник РГМУ

View full text Add to dashboard Cite

Sredi onkoliticheskih virusov odnim iz naibolee izuchennyh yavlyaetsya virus ospovakciny (VV), shtamma modificirovannogo vysokoattenuirovannogo virusa Ankara (MVA), pokazavshego mnogoobeshchayushchie rezul'taty v doklinicheskih i klinicheskih ispytaniyah. SHtamm Lister VV iz Moskovskogo Instituta virusnyh preparatov (LIVP) issledovan v men'shej stepeni, chem MVA i imeet otlichnyj ot MVA tropizm. Cel'yu raboty bylo sravnit' onkoliticheskuyu effektivnost' shtammov LIVP i MVA v otnoshenii solidnyh opuholej. Dlya povysheniya selektivnosti LIVP i MVA k opuholevym kletkam nami byli polucheny rekombinantnye varianty s inaktivaciej gena timidinkinazy (TK), MVA-RFP i LIVP-RFP, ekspressiruyushchie krasnyj fluorescentnyj belok. Kinetiku replikacii i onkoliticheskuyu aktivnost' poluchennyh rekombinantnyh shtammov ocenivali in vitro i in vivo na liniyah opuholevyh kletok i allotransplantatah myshinyh singennyh modelej metastaticheskoj adenokarcinomy molochnoj zhelezy myshi 4T1, adenokarcinomy tolstoj kishki CT26 i melanomy B16. Kak MVA-RFP, tak i LIVP-RFP pokazali vysokuyu effektivnost' replikacii v opuholevyh kletkah i vyrazhennuyu onkoliticheskuyu aktivnost' v otnoshenii allotransplantatov melanomy V16 i adenokarcinomy molochnoj zhelezy 4T1. V otnoshenii 4T1, yavlyayushchejsya model'yu trojnogo negativnogo raka molochnoj zhelezy cheloveka, LIVP-RFP po sravneniyu s MVA-RFP pokazal bolee chem na 50% povyshennuyu citotoksichnost' v testah in vitro, a takzhe dostovernoe zamedlenie progressirovaniya allotransplantatov 4T1 i povyshenie vyzhivaemosti zhivotnyh v eksperimentah in vivo. Primenenie shtamma LIVP v kachestve platformy pri razrabotke rekombinantnyh onkoliticheskih virusov dlya terapii raka molochnoj zhelezy mozhet byt' bolee perspektivnym, chem primenenie shtamma MVA.

show abstract

Section: Bulletin Of Rsmu 2 2023 Vestnikrgmuru | |unclassified

Sravnenie onkoliticheskoj aktivnosti rekombinantnyh shtammov virusa ospovakciny LIVP-RFP i MVA-RFP v otnoshenii solidnyh opuholej

Шакиба¹,

Набережная²,

Кочетков³

et al. 2023

Вестник РГМУ

View full text Add to dashboard Cite

show abstract

“…Vaccinia virus Lister strain from the Institute of Virus Preparation, Moscow, Russia (LIVP) is an attenuated variant reported to hold excellent oncolytic properties. 18 , 19 , 20 , 21 Previously, it was demonstrated that this strain could treat highly immunosuppressive tumors such as triple-negative breast cancer (TNBC) models. 22 Therefore, we utilized this strain for further modification and development of recombinant variants expressing murine IL-15 (LIVP-IL15-RFP) or murine IL-15Rα (LIVP-IL15Ra-RFP) with deletion of thymidine kinase (TK) to treat 4T1 murine breast cancer model, which represents a highly immunosuppressive tumor model, and CT26 murine colon carcinoma.…”

Section: Introductionmentioning

confidence: 99%

Oncolytic therapy with recombinant vaccinia viruses targeting the interleukin-15 pathway elicits a synergistic response

Shakiba

Vorobyev

Yusubalieva

et al. 2023

Molecular Therapy - Oncolytics

View full text Add to dashboard Cite

“…Advances in massive sequencing technologies over the past decade have allowed for a better understanding of the genetic relationships between different smallpox vaccines [ 11 , 12 , 13 , 14 , 15 , 16 , 17 , 18 , 19 , 20 , 21 ]. In this context, the complete genomic sequencing of Lister, IOC and Dryvax vaccine strains showed high genetic diversity among clonal isolates obtained from a single vaccine vial, especially in the genome ends where virulence genes are located [ 12 , 17 , 20 , 21 ].…”

Section: Introductionmentioning

confidence: 99%

Genomic Characterization of the Historical Smallpox Vaccine Strain Wyeth Isolated from a 1971 Seed Vial

Cruz

Luques

Castiñeiras

et al. 2022

Viruses

View full text Add to dashboard Cite

The Wyeth strain of vaccinia virus (VACV) produced by Wyeth Pharmaceuticals was supposedly used to manufacture the old freeze-dried American smallpox vaccine, Dryvax, until its discontinuation in 2008. Although the genomic sequences of numerous Dryvax clones have been reported, data on VACV-Wyeth genomes are still lacking. Genomic analysis of old VACV strains is relevant to understand the evolutionary relationships of smallpox vaccines, particularly with the recent resumption of smallpox vaccination in certain population groups as an attempt to control the worldwide monkeypox outbreak. Here we analyzed the complete genome sequences of three VACV-Wyeth clonal isolates obtained from a single seed vial donated to the Brazilian eradication program in the 1970s. Wyeth clones show >99.3% similarity to each other and >95.3% similarity with Dryvax clones, mapping together in clade I of the vaccinia group. Although the patterns of SNPs and INDELs comparing Dryvax and Wyeth clones are overall uniform, important differences were detected particularly at the ends of the genome. In addition, we detected recombinant events of clone Wyeth A111 and the Dryvax clone Acam2000, suggesting that other regions of the genomes may have similar patchy patterns of recombination. A small-scale serological survey using VACV-Wyeth as antigen in ELISA assays revealed that 63 of the 65 individuals born before the end of smallpox vaccination in Brazil still have anti-VACV IgG antibodies, demonstrating the usefulness of the VACV-Wyeth strain in future extended serological studies of the Brazilian population.

show abstract

Complete Genome Sequence of Vaccinia Virus Strain L-IVP

Cited by 7 publications

References 6 publications

Sravnenie onkoliticheskoj aktivnosti rekombinantnyh shtammov virusa ospovakciny LIVP-RFP i MVA-RFP v otnoshenii solidnyh opuholej

Sravnenie onkoliticheskoj aktivnosti rekombinantnyh shtammov virusa ospovakciny LIVP-RFP i MVA-RFP v otnoshenii solidnyh opuholej

Oncolytic therapy with recombinant vaccinia viruses targeting the interleukin-15 pathway elicits a synergistic response

Genomic Characterization of the Historical Smallpox Vaccine Strain Wyeth Isolated from a 1971 Seed Vial

Contact Info

Product

Resources

About