Complete Genome Sequence of Vibrio anguillarum Phage CHOED Successfully Used for Phage Therapy in Aquaculture

Romero, Jaime; Higuera, Gastón; Gajardo, Felipe; Castillo, Daniel; Middleboe, Mathias; García, Katherine; Espejo, Romilio T.

doi:10.1128/genomea.00091-14

Cited by 9 publications

(8 citation statements)

References 8 publications

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“…The CHOED bacteriophage that naturally infects the V. anguillarum PF4 strain is a lytic phage belonging to the Podoviridae family and was isolated from molluscs in southern Chile (Higuera et al, 2013). Its genome has been sequenced previously (Romero et al, 2014).…”

Section: Methodsmentioning

confidence: 99%

Diversification of Vibrio anguillarum Driven by the Bacteriophage CHOED

et al. 2019

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Bacteriophages are an important factor in bacterial evolution. Some reports suggest that lytic bacteriophages can select for resistant mutant strains with reduced virulence. The present study explores the role of the CHOED bacteriophage in the diversification and virulence of its host Vibrio anguillarum . Nine phage-resistant strains were analyzed for their phenotype and different virulence factors, showing alterations in their fitness, motility, biofilm formation, lipopolysaccharide profiles and/or protease activity. Seven of the nine phage-resistant strains showed virulence reduction in a Sparus aurata larvae model. However, this is not generalized since two of the resistant strains show equal virulence compared with the parental strain. The genomic analysis of representative resistant strains displayed that the majority of the mutations are specific for each isolate, affecting genes related to lipopolysaccharide biosynthesis, quorum sensing, motility, toxin and membrane transport. The observed mutations were coherent with the phenotypic and virulence differences observed. These results suggest that the CHOED phage acts as a selective pressure on V. anguillarum , allowing proliferation of resistant strains with different genotypes, phenotypes and degrees of virulence, contributing to bacterial diversification.

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Section: Methodsmentioning

confidence: 99%

Diversification of Vibrio anguillarum Driven by the Bacteriophage CHOED

et al. 2019

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“…Romero et al . () demonstrated that Chilean mussels naturally produced V. anguillarum ‐specific Podoviridae ‐phage CHOED in situ and in vitro (Higuera et al . ).…”

Section: Closing Remarksmentioning

confidence: 97%

“…Ultimately with time, aquaculture will stray from its present practices and evolve to more resemble natural, functional marine habitats. Romero et al (2014) demonstrated that Chilean mussels naturally produced V. anguillarum-specific Podoviridae-phage CHOED in situ and in vitro (Higuera et al 2013). If facilities cultured such submerged organisms as Chilean mussels in the bottom of each aquaculture tank, healthy fish masses could be produced naturally without the need for commercialized chemicals.…”

Section: Future Researchmentioning

confidence: 99%

A comprehensive review of Vibrio (Listonella) anguillarum: ecology, pathology and prevention

Hickey

Lee

2017

Reviews in Aquaculture

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Vibrio (Listonella) anguillarum is a marine bacteria that is pathogenic to a number of aquatic organisms including several species which are important to the aquaculture industry. Organisms that are infected by V. anguillarum are diagnosed with vibriosis which can be lethal in a matter of days. Vibriosis can be particularly devastating to aquaculture businesses and measures of prevention or treatment are generally quite expensive. Efforts to understand and control V. anguillarum virulence have been of high‐priority among international aquatic research studies. The knowledge that has accumulated as a result of this collective research effort is reviewed in this article.

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“…Studies on V. anguillarum -specific bacteriophages have so far mainly focused on lytic phages and their potential use in phage therapy application [ 30 , 31 , 32 ] while very little is known about the role of temperate phage-encoded virulence and other fitness factors as drivers of functional dynamics in V. anguillarum . Improving our understanding of how phages may influence the genomic and phenotypic characteristics of V. anguillarum thus requires more detailed knowledge of the genetic properties and dispersal of temperate phages infecting this pathogen as well as their distribution as prophages in the host community.…”

Section: Introductionmentioning

confidence: 99%

Stumbling across the Same Phage: Comparative Genomics of Widespread Temperate Phages Infecting the Fish Pathogen Vibrio anguillarum

Kalatzis

Rørbo

Castillo

et al. 2017

Viruses

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Nineteen Vibrio anguillarum-specific temperate bacteriophages isolated across Europe and Chile from aquaculture and environmental sites were genome sequenced and analyzed for host range, morphology and life cycle characteristics. The phages were classified as Siphoviridae with genome sizes between 46,006 and 54,201 bp. All 19 phages showed high genetic similarity, and 13 phages were genetically identical. Apart from sporadically distributed single nucleotide polymorphisms (SNPs), genetic diversifications were located in three variable regions (VR1, VR2 and VR3) in six of the phage genomes. Identification of specific genes, such as N6-adenine methyltransferase and lambda like repressor, as well as the presence of a tRNAArg, suggested a both mutualistic and parasitic interaction between phages and hosts. During short term phage exposure experiments, 28% of a V. anguillarum host population was lysogenized by the temperate phages and a genomic analysis of a collection of 31 virulent V. anguillarum showed that the isolated phages were present as prophages in >50% of the strains covering large geographical distances. Further, phage sequences were widely distributed among CRISPR-Cas arrays of publicly available sequenced Vibrios. The observed distribution of these specific temperate Vibriophages across large geographical scales may be explained by efficient dispersal of phages and bacteria in the marine environment combined with a mutualistic interaction between temperate phages and their hosts which selects for co-existence rather than arms race dynamics.

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Complete Genome Sequence of Vibrio anguillarum Phage CHOED Successfully Used for Phage Therapy in Aquaculture

Cited by 9 publications

References 8 publications

Diversification of Vibrio anguillarum Driven by the Bacteriophage CHOED

Diversification of Vibrio anguillarum Driven by the Bacteriophage CHOED

A comprehensive review of Vibrio (Listonella) anguillarum: ecology, pathology and prevention

Stumbling across the Same Phage: Comparative Genomics of Widespread Temperate Phages Infecting the Fish Pathogen Vibrio anguillarum

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