Complete Genome Sequence of West Nile Virus (WNV) from the First Human Case of Neuroinvasive WNV Infection in Cyprus

Richter, Jan; Tryfonos, Christina; Tourvas, Aristomenis; Floridou, Dora; Paphitou, Niki I.; Christodoulou, Christina

doi:10.1128/genomea.01110-17

Cited by 17 publications

(12 citation statements)

References 10 publications

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“…The phylogenetic tree clearly demonstrates that all five samples obtained from humans clustered with the eastern European subtype of WNV lineage I, cluster 2 (Figure 3). Interestingly, the most closely related WNV reference strain was isolated from the first human case of WNV infection diagnosed in Cyprus in August 2016 [9,10]. WNV strains found in mosquitoes belonged to both the Mediterranean and eastern European subtypes of WNV lineage I, cluster 2.…”

Section: Phylogenetic Investigationmentioning

confidence: 99%

Epidemiologic and phylogenetic analysis of the 2018 West Nile virus (WNV) outbreak in Israel demonstrates human infection of WNV lineage I

et al. 2019

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As at 12 November 2018, an outbreak of West Nile virus (WNV) was responsible for 139 WNV infection cases in Israel. Here, we characterise the epidemiology of the outbreak and demonstrate that only WNV lineage I was circulating in mosquitoes and responsible for WNV infection in humans. This suggests that the concurrence of the outbreak in Israel with WNV outbreaks in several European countries is not due to a common, more virulent WNV genotype.

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Section: Phylogenetic Investigationmentioning

confidence: 99%

Epidemiologic and phylogenetic analysis of the 2018 West Nile virus (WNV) outbreak in Israel demonstrates human infection of WNV lineage I

et al. 2019

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“…Undoubtedly, detection of novel virus variants and species has been facilitated by the dramatic progress in whole-genome sequencing [46][47][48][49][50][51], thanks to the advent of a number of next-generation sequencing methods allowing full-length sequencing at a fraction of the time and cost required for traditional Sanger sequencing [52].…”

Section: Sequence Of Target Rna Virusesmentioning

confidence: 99%

Targeting the RdRp of Emerging RNA Viruses: The Structure-Based Drug Design Challenge

et al. 2020

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The RNA-dependent RNA polymerase (RdRp) is an essential enzyme for the viral replication process, catalyzing the viral RNA synthesis using a metal ion-dependent mechanism. In recent years, RdRp has emerged as an optimal target for the development of antiviral drugs, as demonstrated by recent approvals of sofosbuvir and remdesivir against Hepatitis C virus (HCV) and severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), respectively. In this work, we overview the main sequence and structural features of the RdRp of emerging RNA viruses such as Coronaviruses, Flaviviruses, and HCV, as well as inhibition strategies implemented so far. While analyzing the structural information available on the RdRp of emerging RNA viruses, we provide examples of success stories such as for HCV and SARS-CoV-2. In contrast, Flaviviruses’ story has raised attention about how the lack of structural details on catalytically-competent or ligand-bound RdRp strongly hampers the application of structure-based drug design, either in repurposing and conventional approaches.

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“…WNV aligns into 7 lineages on the basis of nucleic acid homology with the dominant lineages deviating by the nucleotide differences with 25% to 30% (Richter et al 2017). Lineage 1 WNV has a worldwide circulation including Africa, Europe, Australia, Asia, North, Central America and the Middle East while lineage 2 is limited to Africa which infrequently progressing to severe disease and can cause mild febrile illness in human (Pattan et al 2009).…”

Section: Lineage Of Wnvmentioning

confidence: 99%

West Nile Virus Infection in Human and Animals: Potential Risks in Malaysia

Yuseri¹,

Yasmin²,

Omar³

et al. 2019

JSM

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West Nile virus (WNV) is a zoonotic RNA virus maintained in enzootic cycles involving mosquito mainly Culex and Aedes spp. as the vector and birds as the reservoir host. WNV is endemic in Africa, Europe and Western Asia. Human infection results in asymptomatic illness such as fever, headache, tiredness, body aches, nausea, vomiting, skin rash, swollen lymph glands and neuroinvasive disease are seen in less than 1% of infected persons. The spectrum of symptoms in animals includes fever, weakness and paralysis of hind limbs, impaired vision, ataxia, head pressing, aimless wandering, seizures, inability to swallow, walking in circles and coma. Based on the previous study in Malaysia, the antibody and nucleic acid against WNV were detected in Orang Asli, captive birds and horses. In this paper, potential risk factors contributing to WNV occurrence are discussed. How the disease or infection is diagnosed and controlled was also discussed.

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Complete Genome Sequence of West Nile Virus (WNV) from the First Human Case of Neuroinvasive WNV Infection in Cyprus

Abstract: We report here the complete genome sequence of a West Nile virus (WNV) strain from the first laboratory-confirmed human case of neuroinvasive WNV infection in Cyprus. Phylogenetic analysis showed that this WNV strain grouped clearly into genetic lineage 1, clade 1a, cluster 2.

Cited by 17 publications

References 10 publications

Epidemiologic and phylogenetic analysis of the 2018 West Nile virus (WNV) outbreak in Israel demonstrates human infection of WNV lineage I

Epidemiologic and phylogenetic analysis of the 2018 West Nile virus (WNV) outbreak in Israel demonstrates human infection of WNV lineage I

Targeting the RdRp of Emerging RNA Viruses: The Structure-Based Drug Design Challenge

West Nile Virus Infection in Human and Animals: Potential Risks in Malaysia

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