Complete Genome Sequences of 11
            <i>Staphylococcus</i>
            sp. Strains Isolated from Buffalo Milk and Milkers’ Hands

Pizauro, Lucas José Luduverio; Almeida, Camila Chioda de; Gohari, Iman Mehdizadeh; MacInnes, Janet I.; Zafalon, L. F.; Kropinski, Andrew M.; Varani, Alessandro M.

doi:10.1128/mra.01264-19

Cited by 5 publications

(6 citation statements)

References 11 publications

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“…According to a comparative sequence analysis, the structure of SCC mec VIII was highly similar to that of SCC mec -CI in S. hominis 19A isolates from buffalo milk, which had ccrA4B4 but lacked the mec gene complex ( 22 ) ( Fig. 2B ).…”

Section: Resultsmentioning

confidence: 84%

“…Antimicrobial resistance genes, including aminoglycoside, tetracycline, and trimethoprim-sulfamethoxazole resistance genes coexisting on the SCC mec element, are important because they may limit the therapeutic options available in veterinary medicine. Related medications are commonly used to treat small animals in Thailand; therefore, coselection with SCC mec might have occurred in 48 and 384 ( 22 ). The presence of heavy metal resistance genes on SCC mec , including zinc, copper, arsenic, and cadmium resistance genes, suggests that they may have originated in CoNS ( 16 ).…”

Section: Discussionmentioning

confidence: 99%

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Novel Organization of the Staphylococcal Cassette Chromosome mec Composite Island in Clinical Staphylococcus haemolyticus and Staphylococcus hominis Subspecies hominis Isolates from Dogs

et al. 2022

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In Thailand, the staphylococcal cassette chromosome mec (SCC mec ) element, which causes methicillin resistance through acquisition of the mec gene, has been studied in clinical coagulase-negative Staphylococcus isolates from various companion animals, and Staphylococcus haemolyticus and Staphylococcus hominis subsp. hominis were found to have the most nontypeable (NT)-SCC mec elements. These species are more prone to causing illness and more resistant to a variety of antimicrobials than other coagulase-negative staphylococci.

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Section: Resultsmentioning

confidence: 84%

Section: Discussionmentioning

confidence: 99%

Novel Organization of the Staphylococcal Cassette Chromosome mec Composite Island in Clinical Staphylococcus haemolyticus and Staphylococcus hominis Subspecies hominis Isolates from Dogs

et al. 2022

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“…[ 46 ]. S. chromogenes strain 34B, methicillin-resistant microorganism isolated from milk samples of Milking Parlor 2, was previously characterized from milk samples of buffaloes affected by subclinical mastitis [ 47 ].…”

Section: Resultsmentioning

confidence: 99%

Exposure of Buffalo Milkers to Pathogenic Bacteria and Characterization of Isolated Methicillin-Resistant Staphylococcus spp.

Carraturo

Alterisio

Guccione

et al. 2022

IJERPH

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The research was focused on the surveillance of the exposure of buffalo milkers in contact with both animals and potentially contaminated equipment, pointing attention on the diffusion of antibiotic-resistant Staphylococcus spp. The monitoring was performed for 12 months, allowing the collection of 600 raw milk and buffalo udder surface samples, 192 milking lanes, 400 milking clusters, 160 personal protective equipment (PPEs) and electronic devices surface samples in contact with the workers of four milking parlors located in Southern Italy. The analysis of the milk samples evidenced the highest exposure to the bacteria considered (and mainly to S. aureus) from late winter–spring seasons onward. The possible risk arising from buffalo udder, milking clusters, and lines were instead considered rather stable along the entire period of sampling. The PPEs turned out to be a source of contamination for milkers mainly during the spring and summer periods. The analysis for oxacillin/methicillin resistance revealed in all the farms enrolled an overall amount of 37.5% of Staphylococci strains (belonging to S. aureus, S. haemolyticus, S. pseudintermedius, S. chromogenes species) resistant both to methicillin and oxacillin. The investigation demonstrated that the potential transfer of pathogenic bacteria to humans would have a better chance to occur at milk resumption time (since late winter–spring onward) when the number of animals to be milked is greater and the activity in the milking parlor is more challenging. At the same time, the findings seem to point out that the potential risk may be worsened by a significant presence of oxacillin/methicillin-resistant Staphylococci, potentially resulting from irrational use of antibiotics.

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“…It was assumed that mastitis states were classified according to the clinical presentation and standard triage test described by Radostits et al 3 . Genomes in this study were from bacteria isolated in Brazil 45 , Canada 46 India, Netherlands, and United States (information regarding isolates BioSample available on Supplementary Table 1 ).…”

Section: Methodsmentioning

confidence: 99%

Genomic comparisons and phylogenetic analysis of mastitis-related staphylococci with a focus on adhesion, biofilm, and related regulatory genes

Pizauro

Almeida

Silva

et al. 2021

Sci Rep

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Mastitis is a common and costly disease on dairy farms, commonly caused by Staphylococcus spp. though the various species are associated with different clinical outcomes. In the current study, we performed genomic analyses to determine the prevalence of adhesion, biofilm, and related regulatory genes in 478 staphylococcal species isolated from clinical and subclinical mastitis cases deposited in public databases. The most prevalent adhesin genes (ebpS, atl, pls, sasH and sasF) were found in both clinical and subclinical isolates. However, the ebpS gene was absent in subclinical isolates of Staphylococcus arlettae, S. succinus, S. sciuri, S. equorun, S. galinarum, and S. saprophyticus. In contrast, the coa, eap, emp, efb, and vWbp genes were present more frequently in clinical (vs. subclincal) mastitis isolates and were highly correlated with the presence of the biofim operon (icaABCD) and its transcriptional regulator, icaR. Co-phylogenetic analyses suggested that many of these adhesins, biofilm, and associated regulatory genes could have been horizontally disseminated between clinical and subclinical isolates. Our results further suggest that several adhesins, biofilm, and related regulatory genes, which have been overlooked in previous studies, may be of use for virulence profiling of mastitis-related Staphylococcus strains or as potential targets for vaccine development.

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Complete Genome Sequences of 11 Staphylococcus sp. Strains Isolated from Buffalo Milk and Milkers’ Hands

Cited by 5 publications

References 11 publications

Novel Organization of the Staphylococcal Cassette Chromosome mec Composite Island in Clinical Staphylococcus haemolyticus and Staphylococcus hominis Subspecies hominis Isolates from Dogs

Novel Organization of the Staphylococcal Cassette Chromosome mec Composite Island in Clinical Staphylococcus haemolyticus and Staphylococcus hominis Subspecies hominis Isolates from Dogs

Exposure of Buffalo Milkers to Pathogenic Bacteria and Characterization of Isolated Methicillin-Resistant Staphylococcus spp.

Genomic comparisons and phylogenetic analysis of mastitis-related staphylococci with a focus on adhesion, biofilm, and related regulatory genes

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