Complete Genome Sequences of Lactobacillus Strains C25 and P38, Isolated from Chicken Cecum

Abstract

We report the complete circular genome sequences of Lactobacillus crispatus strain C25, its plasmid, and Lactobacillus animalis strain P38; both strains were isolated from the cecum of 4-week-old chickens. These isolates represent potential probiotic strains for poultry.

Complete Genome Sequences of Six Lactobacilli Isolated from American Quarter Horses

“…Gram-positive, catalase-negative, non-spore formers and nonmotile colonies were selected, grown in MRS, and stored at −80°C in MRS/25% glycerol. Frozen cells were cultured anaerobically in MRS for 18 h. The steps of DNA extraction through sequencing have been described previously ( 5 ).…”

“…The assemblies were generated using HGAP4 (SMRT Link v5.1.0) ( 6 ). Preliminary assemblies showed high coverage (>2,000×), and the reads were subsampled as described in reference 5 (with sampling fractions listed in Table 1 ) before generating the assemblies. Circlator v1.5.5 (assembler, Canu) ( 7 ) was used for circularization, and the assemblies were polished with the subsampled reads using Arrow (GenomicConsensus package v2.2.2 in SMRT Link).…”