Complete Genomic Sequence of Duck Flavivirus from China

Liu, Ming; Liu, Chunguo; Li, Gang; Li, Xiaojun; Yin, Xiuchen; Chen, Yuhuan; Zhang, Yun

doi:10.1128/jvi.07086-11

Cited by 35 publications

(32 citation statements)

References 11 publications

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“…To date, complete sequences of 14 DEDSV strains, including two strains isolated from geese, one from a pigeon, and the other 11 from ducks, have been released in GenBank (Table 1). While the genomes of 14 of the 15 strains are of the same length (10990 nt), the strain TA genome is shorter by 4 nt [30] . Thus, we suspect that its genome has not been fully sequenced.…”

Section: The Virusmentioning

confidence: 99%

“…The lengths of the predicted cleavage proteins are as follows: capsid (C), 120 aa; premembrane (PrM), 167 aa; envelope (E), 501 aa; nonstructural protein 1 (NS1), 352 aa; NS2A, 227 aa; NS2B, 131 aa; NS3, 619 aa; NS4A, 126 aa; 2K, 23 aa; NS4B, 254 aa; and NS5, 905 aa [15,25]. However, Liu et al [30] report a different prediction, where the PrM is longer, and NS1 is shorter (the difference in each is 10 aa). Another report from Diao's group includes an absolutely distinct prediction, in which most of the cleavage sites are not identical to other predictions [19].…”

Section: The Virusmentioning

confidence: 99%

“…The complete ORF, flanked by 5′-and 3′-NCRs of 94 and 618 nt, respectively, is predicted to encode a large polyprotein of 3425 amino acid (aa). After co-and post-translational cleavage by host signalase or viral serine protease, the polyprotein gives rise to 10 viral proteins, including three structural proteins (C, PrM, and E) and seven nonstructural proteins (NS1, NS2a, NS2b, NS3, NS4a, NS4b, and NS5), which is typical of flaviviruses [1519, 25,30].…”

Section: The Virusmentioning

confidence: 99%

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Duck egg drop syndrome virus: an emerging Tembusu-related flavivirus in China

Lü

et al. 2013

Sci. China Life Sci.

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Duck egg drop syndrome virus (DEDSV) is a newly emerging pathogenic flavivirus isolated from ducks in China. DEDSV infection mainly results in severe egg drop syndrome in domestic poultry, which leads to huge economic losses. Thus, the discovery of ways and means to combat DEDSV is urgent. Since 2010, a remarkable amount of progress concerning DEDSV research has been achieved. Here, we review current knowledge on the epidemiology, symptomatology, and pathology of DEDSV. A detailed dissection of the viral genome and polyprotein sequences, comparative analysis of viral antigenicity and the corresponding potential immunity against the virus are also summarized. Current findings indicate that DEDSV should be a distinct species from Tembusu virus. Moreover, the adaption of DEDSV in wildlife and its high homology to pathogenic flaviviruses (e.g., West Nile virus, Japanese encephalitis virus, and dengue virus), illustrate its reemergence and potential to become a zoonotic pathogen that should not be overlooked. Detailed insight into the antigenicity and corresponding immunity against the virus is of clear significance for the development of vaccines and antiviral drugs specific for DEDSV.

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Section: The Virusmentioning

confidence: 99%

Section: The Virusmentioning

confidence: 99%

Section: The Virusmentioning

confidence: 99%

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Duck egg drop syndrome virus: an emerging Tembusu-related flavivirus in China

Lü

et al. 2013

Sci. China Life Sci.

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“…Complete genomic sequences of DTMUV isolated in eastern China have been reported (2,4). However, there has been no report of a complete genomic sequence of DTMUV in southern China's important waterfowlproducing areas, where the majority of production takes place on small-scale farms with low biosecurity levels.…”

mentioning

confidence: 99%

Complete Genome Sequence of Duck Tembusu Virus, Isolated from Muscovy Ducks in Southern China

Zhu

Chen

Wei

et al. 2012

J Virol

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We report here the complete genomic sequence of the duck Tembusu virus (DTMUV) WJ-1 strain, isolated from Muscovy ducks. This is the first complete genome sequence of DTMUV reported in southern China. Compared with the other strains (TA, GH-2, YY5, and ZJ-407) that were previously found in eastern China, WJ-1 bears a few differences in the nucleotide and amino acid sequences. We found that there are 47 mutations of amino acids encoded by the whole open reading frame (ORF) among these five strains. The whole-genome sequence of DTMUV will help in understanding the epidemiology and molecular characteristics of duck Tembusu virus in southern China.

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“…The 5=-terminal and 3=-terminal sequences were determined by using a 5=-Full RACE kit and a 3=-Full RACE core set kit, version 2.0 (TaKaRa, Dalian, China), as described by Liu et al (5). All fragments were cloned into pMD-18T vector (TaKaRa) and sequenced in bothdirectionsintriplicate.Theclonedplasmidsweresequencedwithan ABI (Applied Biosystems) 3730 DNA analyzer.…”

mentioning

confidence: 99%

Complete Genome Sequence of Avian Tembusu-Related Virus Strain WR Isolated from White Kaiya Ducks in Fujian, China

Wan

Huang

et al. 2012

J Virol

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Avian tembusu-related virus, which was first identified in China, is an emerging virus causing serious economic loss to the Chinese poultry industry. We report here the complete genome sequences of avian tembusu-related virus strain WR, isolated from a White Kaiya duck with disease characterized by an abrupt decrease in egg laying with ovarian hemorrhage, which will help in further understanding the molecular and evolutionary characteristics and pathogenesis of avian tembusu-related virus, the new flavivirus affecting ducks in Southern China. Since April 2010, a large-scale outbreak of disease characterized by an abrupt decrease in egg laying with ovarian hemorrhage, with nearly 100% morbidity but 0 to 12% mortality in infected duck farms, has been observed in nearly 13 coastal provinces in China, such as Hebei, Shandong, Zhejiang, Fujian, Guangdong, and Guangxi, and in nearly all duck species and breeds, such as Beijing duck, muscovy duck, Cheery Valley duck, and some Chinese traditional breeds (1, 6, 9). The causative agent was first identified by us, using the same strategy described by Wang et al. (8), and deposited in GenBank under accession number HQ828066 (7). We also first identified the new tembusu-related flavivirus as infecting domestic layer hens in Fujian, China (2). Now it has been identified as a new flavivirus-duck tembusu-related (or tembusu-like) virus (4) (6), and research showing that the virus may be infecting chickens and geese indicates that this new flavivirus has adapted to domestic avian species (7). Therefore, we suggest naming this new flavivirus avian tembusurelated virus (ATrV). We determined the complete genome sequence of avian tembusu-related virus strain WR from White Kaiya ducks; the strain was isolated in this outbreak by using 10-day-old muscovy duck embryonated eggs.Based on the sequences of flavivirus Bagaza virus (AY632545) (3)and duck tembusu-related flavivirus strains TA (5) and YY5 (10), 12 pairs of oligonucleotide primers were designed to amplify the different regions of the strain WR genome with overlapping genome fragments covering each fragment. The 5=-terminal and 3=-terminal sequences were determined by using a 5=-Full RACE kit and a 3=-Full RACE core set kit, version 2.0 (TaKaRa, Dalian, China), as described by Liu et al. (5). All fragments were cloned into pMD-18T vector (TaKaRa) and sequenced in bothdirectionsintriplicate.Theclonedplasmidsweresequencedwithan ABI (Applied Biosystems) 3730 DNA analyzer. All of the sequences obtained were assembled using the SeqMan programs (DNASTAR; Lasergene) to generate the contiguous full-genome sequences. The complete genome sequence of strain WR is 10,990 nucleotides (nt) in length with a typical flavivirus genome organization. The predicted polyprotein, encoded by a single open reading frame (10,278 nt) flanked by a 94-nt-long 5= untranslated region (5= UTR) and a 618-nt-long 3= UTR, comprises a leader protein, three structural proteins (capsid, preM, and envelope), and five nonstructural proteins (NS1 to NS5).Compared to ...

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Complete Genomic Sequence of Duck Flavivirus from China

Cited by 35 publications

References 11 publications

Duck egg drop syndrome virus: an emerging Tembusu-related flavivirus in China

Duck egg drop syndrome virus: an emerging Tembusu-related flavivirus in China

Complete Genome Sequence of Duck Tembusu Virus, Isolated from Muscovy Ducks in Southern China

Complete Genome Sequence of Avian Tembusu-Related Virus Strain WR Isolated from White Kaiya Ducks in Fujian, China

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