Complete mitochondrial genome sequence ofLabriocimbex sinicus, a new genus and new species of Cimbicidae (Hymenoptera) from China

Abstract

Labriocimbex sinicus Yan & Wei gen. et sp. nov. of Cimbicidae is described. The new genus is similar to Praia Andre and Trichiosoma Leach. A key to extant Holarctic genera of Cimbicinae is provided. To identify the phylogenetic placement of Cimbicidae, the mitochondrial genome of L. sinicus was annotated and characterized using high-throughput sequencing data. The complete mitochondrial genome of L. sinicus was obtained with a length of 15,405 bp (GenBank: MH136623; SRA: SRR8270383) and a typical set of 37 genes (22 tRNAs, 13 PCGs, and two rRNAs). The results demonstrated that all PCGs were initiated by ATN codon, and ended with TAA or T stop codons. The study reveals that all tRNA genes have a typical clover-leaf secondary structure, except for trnS1. Remarkably, the secondary structures of the rrnS and rrnL of L. sinicus were much different from those of Corynis lateralis. Phylogenetic analyses verified the monophyly and positions of the three Cimbicidae species within the superfamily Tenthredinoidea and demonstrated a relationship as (Tenthredinidae + Cimbicidae) + (Argidae + Pergidae) with strong nodal supports. Furthermore, we found that the generic relationships of Cimbicidae revealed by the phylogenetic analyses based on COI genes agree quite closely with the systematic arrangement of the genera based on the morphological characters. Phylogenetic tree based on two methods shows that L. sinicus is the sister group of Praia with high support values. We suggest that Labriocimbex belongs to the tribe Trichiosomini of Cimbicinae based on adult morphology and molecular data. Besides, we suggest to promote the subgenus Asitrichiosoma to be a valid genus.

The complete mitochondrial genome of Praia tianmunica (Hymenoptera: Cimbicidae) with related phylogenetic analysis

“…This confirmed the results based on cox1 fragments in Yan et al. ( 2019 ). All related files in this study have been uploaded to figshare ( https://figshare.com/projects/Praiatianmunica/83591 ).…”

“…It revealed the sister-group relationship between P. tianmunica and L. sinicus, and then the two species together as the sister group of Trichiosoma þ Asitrichiosoma. This confirmed the results based on cox1 fragments in Yan et al (2019). All related files in this study have been uploaded to figshare (https://figshare.com/projects/Praiatianmunica/83591).…”

“…A recent cladistic analysis of Cimbicidae based on morphological characters showed that Praia is a basal lineage of Cimbicinae (Vilhelmsen 2019). While Yan et al (2019) proposed Praia was the sister group of Labriocimbex, and close related to Trichiosoma, thus to place Praia into tribe Trichiosomini. In this study, we presented the complete mitochondrial genome of P. tianmunica, and conducted a phylogenetic analysis incorporated with other available sequences, to clarify the phylogenetic position of this genus further.…”

“…Praia Wankowicz, 1880 is a small Palearctic genus of Cimbicidae with four species that have been reported (Yan et al. 2019 ). The systematic position of the genus within Cimbicidae is uncertain at present.…”

Nearly complete mitochondrial genome of Trichiosoma vitellina Linné, 1760 (Hymenoptera: Tenthredinidae): sequencing and phylogenetic analysis

“…An interval region (31 bp) between trnH and nad4 was found by assembling T. vitellina using nad5 (1714 bp) and L. linealis as references. Labriocimbex sinicus Yan et al., 2019 and T. anthracinum Forsius, 1930 were utilized as references with the mean depth of coverage across the sequences of 2901 and 55208, respectively, to verify the above results. Annotations were generated in the MITOS web server (Bernt et al.…”