Complete mitochondrial genomes of two moths in the tribe Trichaeini (Lepidoptera: Crambidae) and their phylogenetic implications

Abstract

The complete mitochondrial genomes of two Prophantis species in the tribe Trichaeini (Lepidoptera: Crambidae) were sequenced using high-throughput sequencing technology. They were assembled and annotated: The complete mitogenomes of P. octoguttalis and P. adusta were 15,197 and 15,714 bp, respectively, and contain 13 protein-coding genes (PCGs), 22 transfer RNA genes, two ribosomal RNA genes, and an A + T-rich region. Their arrangement was consistent with the first sequenced mitogenome of Bombyx mori (Bombycidae) in Lepidoptera, which had the trnM-trnI-trnQ rearrangement. The nucleotide composition was obviously AT-biased, and all PCGs, except for the cox1 gene (CGA), used ATN as the start codon. Except for trnS1, which lacked the DHU stem, all tRNA genes could fold into the clover-leaf structure.The features of these two mitogenomes were highly consistent with those of other species of Spilomelinae in previous studies. Phylogenetic trees of Crambidae were reconstructed based on mitogenomic data using maximum likelihood and Bayesian inference analysis methods. Results showed that Trichaeini in this study robustly constitute a monophyletic group in Spilomelinae, with the relationships (Trichaeini + N omophilini) + ((Spilomelini + (Hymeniini + Agroterini)) + Margaroniini). However, the affinities of the six subfamilies Acentropinae, Crambinae, Glaphyriinae, Odontiinae, Schoenobiinae, and Scopariinae within the "non-PS Clade" in Crambidae remained doubtful with unstable topologies or low supports.

Complete Mitochondrial Genome of the Eggplant Fruit and Shoot Borer, Leucinodes orbonalis Guenée (Lepidoptera: Crambidae), and Comparison with Other Pyraloid Moths

“…In the CAMMSS, Schoenobiinae was recovered to be a sister to Acentropinae. This result is consistent with earlier mitogenomic works [54,90], but different from others [60,62], which places Schoenobiinae as a sister to Crambinae. The subfamilies represented in the non-PS clade were recovered to be monophyletic, and this is well supported for in both trees (Glaphyriinae: 100% BS; 1 PP, Odontinae: 100% BS; 1 PP, Crambinae: 100% BS, 1 PP, Acentropinae: 99.9% BS; 1 PP) (Figure 5).…”

“…Both are classified under Pyraustinae but were robustly placed in Spilomelinae. It is interesting to note, however, that such placement for the said taxa was also observed in earlier works [54,90]. The earlier work by Tang and Du (2023) shows consistent subfamily relationships with those obtained in Figure 5 but differ greatly in the lower-level relationships within Spilomelinae [90].…”

“…It is interesting to note, however, that such placement for the said taxa was also observed in earlier works [54,90]. The earlier work by Tang and Du (2023) shows consistent subfamily relationships with those obtained in Figure 5 but differ greatly in the lower-level relationships within Spilomelinae [90]. In Figure 5, the tribe Spilomelini is a sister to the clade containing Pycnarmon lactiferalis and Syllepte taiwanalis, and their collective clade is a sister to the tribe Margaroniini, which in Figure 5 is the clade containing the genus Glyphodes and Maruca.…”

“…In Figure 5, the tribe Spilomelini is a sister to the clade containing Pycnarmon lactiferalis and Syllepte taiwanalis, and their collective clade is a sister to the tribe Margaroniini, which in Figure 5 is the clade containing the genus Glyphodes and Maruca. This relationship is concordant with Tang and Du (2023) [90]. However, the relationships within Margaroniini are different.…”

First record of the complete mitochondrial genome of Botyodes diniasalis (Walker, 1859) (Lepidoptera: Crambidae)

“… 2016 ), OQ472987 Herpetogramma luctuosalis (Zhou and Yang 2023a ), KJ739310 Spoladea recurvalis (He et al. 2015 ), OP559507 Prophantis octoguttalis (Tang and Du 2023 ), OQ472990 Patania chlorophanta (Zhou and Yang 2023b ), OQ472986 Haritalodes derogata (Zhao et al. 2016 ).…”