Complete nucleotide sequence of a Japanese isolate of Chrysanthemum virus B (genus Carlavirus)

Abstract

The complete nucleotide sequence of a Chrysanthemum virus B isolate from Japan (CVB-S) has been determined. The genomic RNA of CVB-S is 8,990 nucleotides long, excluding the poly(A) tail and, like that of other carlaviruses, contains six open reading frames (ORFs). Multiple alignment and phylogenetic analyses indicated that the phylogenetic relationship among members of the genus Carlavirus is very diverse, with phlox virus S being the closest relative of CVB. In aphid transmission tests, CVB-S was transmitted at a very low rate by Aphis gossypii, a new vector of the virus.

Viruses infecting main ornamental plants: an overview

“…Symptoms of CVB infection vary from leaf mottling or vein clearing to pronounced mosaic and inflorescences deformation, although asymptomatic infections are also observed. CVB is transmitted from plant to plant by various aphid species and characterized by high genetic diversity (OHKAWA et al, 2007;SINGH et al, 2007). Chrysanthemum plants infected with the PVY strain N-Wilga demonstrated spotting and yellowing symptoms (LIU et al, 2014).…”

First Detection and Characterization of Chrysanthemum virus B infecting Chrysanthemum in Thailand

“…CVB is globally distributed where chrysanthemums are grown and most commercial cultivars are usually infected by CVB without showing visible symptoms, but some cultivars showed mild mosaic, vein clearing or necrotic streak symptoms on leaves and the owers, sometimes, are malformed [3][6] [12]. The transmission of this virus occurs by some aphids consisting Myzus persicae and Aphis gossypii as a non-persistent manner and is also sap-transmissible [13].…”

Chrysanthemum virus B and chrysanthemum chlorotic mottle viroid infect chrysanthemum in South Africa

“…Chrysanthemum virus B (CVB) is a member of the genus Carlavirus and has a widespread distribution among chrysanthemum producing countries. It has a genome size of about 9,000 nt coding for six open reading frames (ORFs), with semiflexuous virions of 685 nm in length (Ohkawa et al 2007) and is vectored by several aphid species, including cosmopolitan Myzus persicae (Hollings 1957). Symptoms of CVB infection typically include mild to severe leaf mosaic and vein aberrations, such as banding and chlorosis (Verma et al 2003) but can also be asymptomatic (Ohkawa et al 2007).…”