Complete Porphyrin Analysis of Pathological Urines

Acute Porphyria. III. The Separation of Preformed Porphyrin and Porphobilinogen in the Urine in Acute Porphyria. A Coproporphyrin Chromogen in Normal and Pathological Urine

“…Three per cent calcium chloride was added in excess to the urine from the acute porphyric and the calcium phosphate was precipitated as described by Sveinsson, Rimington & Barnes (1949). The precipitate was washed with 0.1 N NaOH and distilled water, and the amount of adsorbed uroporphyrin was determined.…”

“…iVorrtay (Submitted for publication January 27, 1951) During the investigation described in the preceding papers (Eriksen, 1951, I and II), it was observed that porphobilinogen was not adsorbed at all or in negligible amounts to calcium phosphate precipitated according to Sveinsson, Riinington 8 Barnes (1949).…”

Sodium and Chloride Depletion in Acute Porphyria With Reference to the Status of Adrenal Cortical Function

“…The adrenocorticotrophic hormone test for adrenal cortical reserve has been fully described by Forsham et al (12). Total urine porphyrin was estimated by adsorption on a calcium phosphate precipitate followed by solution in hydrochloric acid as described by Sveinsson, Rimington and Barnes (13), the final assay of porphyrin being carried out in the fluorimeter.…”

Acute porphyria. I. some observations on the solubility, precipitation, fluorescense and absorbtion spectra of calcium phosphate-adsorbed porphyrin pigments

“…Swine)-, Nicholas Sr Prunty (1950) separated, by means of the paper partition chromatography method of Nicholas and Rimington (1949), porphyriiis with 2, 3, 1, 5, 6, 7 and S carbosyl groups in the urine, feces and liver from 3 acute porphyrics. The porphyrins with 5 , 6, 7 and S carbosyl groups were not extractable with ether/glaciai acetic acid.…”

“…The urinary pigments were then adsorbed to calcium phosphate according to Sveinsson, Rimington & Barnes ( 1949).…”