Complex Binding of the Embryonic Interferon, Ovine Trophoblast Protein-1, to Endometrial Receptors

Hansen, Thomas; Kazemi, Mohammad; Keisler, D.H.; Malathy, P.; Imakawa, Kazuhiko; Roberts, R. Michael

doi:10.1089/jir.1989.9.215

Cited by 74 publications

(40 citation statements)

References 33 publications

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“…The molecular weights of the slower migrating bands for both subunits were calculated to be around 110 000, values similar to those described by others [43,44,50]. The precise nature of the lower molecular weight forms of both subunits, which have been observed previously in cross-linking experiments [41,51], remains mysterious. These forms probably did not arise as a result of proteolysis during processing of the membrane fractions [41].…”

Section: Discussionsupporting

confidence: 80%

“…Each must have at least a partial cytoplasmic domain, because the antisera were raised against these regions of the proteins. The presence of these smaller forms of the receptor might explain why binding studies [31,33,51] have always revealed two populations of receptors in the ovine endometrium, one with a binding affinity in the 10 Ϫ10 M range, the other with a dissociation constant about 10-fold higher. In bovine uterus, where affinity cross-linking has revealed only a single band of ϳ110 kDa, only the high-affinity population of receptors can be detected [41].…”

Section: Discussionmentioning

confidence: 99%

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Expression of Interferon Receptor Subunits, IFNAR1 and IFNAR2, in the Ovine Uterus1

Rosenfeld

Han

Alexenko

et al. 2002

Biology of Reproduction

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Interferon-tau (IFN-tau) is the antiluteolytic factor released by concepti of ruminant ungulate species prior to implantation. All type I interferons, including IFN-tau, exert their action through a common receptor, which consists of two subunits, IFNAR1 and IFNAR2c, but the distribution of the two polypeptides in uterine endometrium has not been examined. In situ hybridization and immunohistochemistry on sections from pregnant and nonpregnant ovine uteri at Days 14 and 15 after estrus and mating showed that both IFNAR1 and IFNAR2 mRNA and protein were strongly expressed in endometrial luminal epithelium (LE), superficial glandular epithelium (GE), and stromal cells, within but not outside caruncles. Similar staining patterns were noted in pregnant and nonpregnant uteri for both subunits. Western blot analysis of membrane fractions from cell lines derived from endometrial LE, GE, and stromal cells, and affinity cross-linking experiments with radioactively labeled IFN-tau performed on crude endometrial membranes indicated the presence of both high ( approximately 110 kDa) and low (75-80 kDa) molecular mass forms of the two receptor subunits. To localize where IFN-tau binds when it is introduced into the uterine lumen, immunohistochemistry with an antiserum against IFN-tau was performed on sections of uteri from Day 14 nonpregnant ewes whose uteri had previously been infused with IFN-tau. Staining was concentrated on the LE and superficial GE cells, and was absent from the deeper regions of the glands and from the stromal tissues. These studies demonstrate the heavy concentration of IFNAR1 and IFNAR2 in cells of the LE and superficial GE, which appear to be the main targets for IFN-tau.

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Section: Discussionsupporting

confidence: 80%

Section: Discussionmentioning

confidence: 99%

Expression of Interferon Receptor Subunits, IFNAR1 and IFNAR2, in the Ovine Uterus1

Rosenfeld

Han

Alexenko

et al. 2002

Biology of Reproduction

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“…The actions of IFNT are mediated by the interferon (alpha and beta) receptor (IFNAR), which is composed of two subunits, IFNAR1 and IFNAR2 (Hansen et al 1989 ). To test the hypothesis that IFNT and its receptor have biological roles in conceptus elongation, an in vivo loss of function study was recently conducted by inhibiting IFNT or IFNAR1/2 mRNA translation in the trophectoderm of the ovine conceptus using morpholino antisense oligonucleotides (MAO) delivered via osmotic pumps from days 8-14 post-mating .…”

Section: Ifnt Regulation Of Endometrial Function and Conceptus Elongamentioning

confidence: 99%

Implantation and Establishment of Pregnancy in Ruminants

Spencer

Hansen

2015

Advances in Anatomy, Embryology and Cell Biology

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The establishment of pregnancy in ruminants occurs during the peri-implantation period and involves the suppression of the endometrial luteolytic mechanism to maintain progesterone production by the corpus luteum (CL). Reciprocal interactions between the elongating conceptus (embryo/fetus and associated extraembryonic membranes) and endometrium culminate in implantation. Antiluteolytic effects of the conceptus are due to the production of interferon tau (IFNT) by the trophoblast that has a paracrine effect to inhibit the upregulation of oxytocin receptors in the endometrial epithelia, thereby disrupting uterine release of luteolytic prostaglandin F2 alpha (PGF) pulses. Additionally, IFNT is released into the uterine vein and has endocrine actions to induce ISGs in peripheral tissues. For example, IFNT may induce luteal resistance to PGF, thereby ensuring survival of the CL and maintenance of pregnancy. Survival of the blastocyst and elongation of the conceptus requires embryotrophic factors from the epithelia of the uterus, and those embryotrophic factors are regulated by ovarian progesterone as well as conceptus-derived factors including IFNT and prostaglandins. This review provides new concepts on mechanisms of the establishment of pregnancy and implantation in ruminants with emphasis on conceptus-maternal signaling associated with elongation of the blastocyst and endometrial responses to the presence of a conceptus.

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“…[1,2]. Bovine trophoblast protein-1 (bTP-1) is a small (22)(23)(24)(25)(26), acidic (pI = 5.7-6.5) polypeptide that is the major polypeptide shown to be synthesized and secreted in vitro by the bovine conceptus during the critical time of maternal recognition of pregnancy (Days [17][18][19][20] [3,4]. Attachment of the bovine conceptus to the uterine endometrium occurs between Days 18 and 24 of gestation [5,6].…”

Section: Introductionmentioning

confidence: 99%

Uterine Cellular Changes in 2′,5′-Oligoadenylate Synthetase during the Bovine Estrous Cycle and Early Pregnancy1

Schmitt

Geisert

Zavy

et al. 1993

Biology of Reproduction

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The signal for maternal recognition of pregnancy in cattle is bovine trophoblast protein-1 (bTP-1), a Type I trophoblast interferon. One of the many functions of interferons is the induction of the 2',5'-oligoadenylate (2-5[A]) system, which is involved in cell division and selective degradation of mRNA. The present study focused on the cellular changes of 2-5(A) synthetase in bovine endometrium during the estrous cycle and early pregnancy. Ability of recombinant bTP-1 to stimulate activity of the enzyme in uterine cells throughout the estrous cycle was also evaluated in vitro. Surface epithelium, glandular epithelium, and stroma were enzymatically separated in cows on Day 5, 10, 15, or 18 postestrus and on Day 15 or 18 of pregnancy. Cell samples were lysed and frozen for determination of the endogenous cellular content of 2-5(A) synthetase. Additional cells obtained during the estrous cycle were cultured and treated with increasing doses of recombinant bovine interferon alpha (rbIFN-alpha) or recombinant bTP-1 (rbTP-1). During the estrous cycle, 2-5(A) synthetase was greatest on Day 5 and declined approximately 10-fold by Day 15 (p < 0.01). Cellular content of 2-5(A) synthetase was similar among all three endometrial cell types. In the gravid horn of pregnant animals, presence of a conceptus significantly increased (p < 0.01) 2-5(A) synthetase in all endometrial cell types compared to levels on Days 15 and 18 of the estrous cycle. On Day 18, levels of 2-5(A) synthetase were 30-fold greater (p < 0.01) in epithelium (surface and glandular) from pregnant cows compared to that from cyclic cows.(ABSTRACT TRUNCATED AT 250 WORDS)

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Complex Binding of the Embryonic Interferon, Ovine Trophoblast Protein-1, to Endometrial Receptors

Cited by 74 publications

References 33 publications

Expression of Interferon Receptor Subunits, IFNAR1 and IFNAR2, in the Ovine Uterus1

Expression of Interferon Receptor Subunits, IFNAR1 and IFNAR2, in the Ovine Uterus1

Implantation and Establishment of Pregnancy in Ruminants

Uterine Cellular Changes in 2′,5′-Oligoadenylate Synthetase during the Bovine Estrous Cycle and Early Pregnancy1

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