Complex DNA mixture analysis in a forensic context: Evaluating the probative value using a likelihood ratio model

Haned, Hinda; Benschop, Corina C.G.; Gill, Peter; Sijen, Titia

doi:10.1016/j.fsigen.2014.11.014

Cited by 43 publications

(35 citation statements)

References 35 publications

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“…The resulting LRs were 3.2 x 10 12 and 128.3 x 10 12 for mixtures from three or four individuals, respectively. This calculation is widely used in forensic interpretations (Ladd et al, 2001;Gill et al, 2006;Haned et al, 2015).…”

Section: Resultsmentioning

confidence: 99%

Detecting multiple DNA human profile from a mosquito blood meal

Rabêlo¹,

Albuquerque²,

Tavares³

et al. 2016

Genet. Mol. Res.

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ABSTRACT. Criminal traces commonly found at crime scenes may present mixtures from two or more individuals. The scene of the crime is important for the collection of various types of traces in order to find the perpetrator of the crime. Thus, we propose that hematophagous mosquitoes found at crime scenes can be used to perform genetic testing of human blood and aid in suspect investigation. The aim of the study was to obtain a single Aedes aegypti mosquito profile from a human DNA mixture containing genetic materials of four individuals. We also determined the effect of blood acquisition time by setting time intervals of 24, 48, and 72 h after the blood meal. STR loci and amelogenin were analyzed, and the results showed that human DNA profiles could be obtained from hematophagous mosquitos at 24 h following the blood meal. It is possible that hematophagous mosquitoes can be used as biological remains at the scene of the crime, and can be used to detect human DNA profiles of up to four individuals.

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Section: Resultsmentioning

confidence: 99%

Detecting multiple DNA human profile from a mosquito blood meal

Rabêlo¹,

Albuquerque²,

Tavares³

et al. 2016

Genet. Mol. Res.

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“…The Federal Bureau of Investigation (FBI) recommends a minimum of 13 STR loci for forensic applications (Budowle et al, 1998;Butler, 2005). The authors Haned et al (2015) reported success in separation and identification of DNA mixture profiles. This type of trace sample has an even greater potential contribution to forensic investigation, because a mosquito could feed on both the victim and offender and both DNA profiles can be analyzed.…”

Section: Resultsmentioning

confidence: 99%

Trace samples of human blood in mosquitoes as a forensic investigation tool

et al. 2015

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Investigations of any type of crime invariably starts at the crime scene by collecting evidence. Thus, the purpose of this research was to collect and analyze an entomological trace from an environment that is similar to those of indoor crime scenes. Hematophagous mosquitoes were collected from two residential units; saliva of volunteers that were residents in the units was also collected for genetic analysis as reference samples. We examined the allele frequencies of 15 short tandem repeat loci (D8S1179, D21S11, D7S820, CSF1PO, D3S1358, TH01, D13S317, D16S539, D2S1338, D19S433, vWA, TPOX, D18S51, D5S818, and FGA) and amelogenin. A total of 26 female hematophagous mosquitoes were identified as Aedes aegypti, Aedes albopictus, and Culex quinquefasciatus; we were able to obtain 11 forensically valid genetic profiles, with a minimum of 0.028203 ng/μL of human DNA. Thus, the results of this study showed that it was possible to correlate human genetic information from mosquitoes with the volunteer reference samples, which validates the use of this information as forensic evidence. Furthermore, we observed mixed genetic profiles from one mosquito. Therefore, it is clearly important to collect these insects indoors where crimes were committed, because it may be possible to find intact genetic profiles of suspects in the blood found in the digestive tract of hematophagous mosquitoes for later comparison to identify an offender and/or exclude suspects

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“…b Determined via maximum allele count method [34]. Selected when 1 contributor (green), maybe when between 2 and 4 contributors (yellow), not selected when 5 or more contributors (red, as such profiles were found too complex to interpret when LT amounts of DNA are involved [8]). c Selected when ≥60% (green), maybe when between 40% and 60% (yellow), not selected when b40% (red).…”

Section: Weight Of Evidence For Samples Comprising Minute Amounts Of Dnamentioning

confidence: 99%

“…STR profiles obtained from minute amounts of DNA generally show only few allele calls and hardly reproduced alleles among amplification replicates [7]. Also, there is often uncertainty on the contributor number [8] and mixture ratio in case multiple contributors are involved. Profiling results for samples with minute amounts of DNA are improved when so called "enhanced interrogation techniques" [9], such as increased cycling or higher capillary electrophoresis (CE) injection settings, are applied [7,[10][11][12][13][14][15][16].…”

Section: Introductionmentioning

confidence: 99%