Complexation behaviors of hyperbranched cationic glycogen derivatives with plasmid DNA revealed by resonance light scattering and circular dichroism spectroscopy

Liu, Xuan; Wu, Simon; Ren, Xianyue; Zhang, Liming; Deng, Yubing; Yang, Liqun

doi:10.1002/star.201400224

Cited by 5 publications

(2 citation statements)

References 28 publications

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“…The constructs exhibited high transfection efficiency, good blood compatibility and low cytotoxicity. The positively charged complexes were formed at a pDNA‐to‐oyster glycogen weight ratio of 2 and functionalized with 3‐(dimethylamino)‐1‐propylamine (DMAPA) moieties and 1‐(2‐aminoethyl)piperazine via carbonyldiimidazole activation . Liu et al investigated the use of oyster glycogen‐mediated complexation of siRNA to target nuclear factor kappa‐light‐chain‐enhancer of activated B cells, NF‐κB, p65 in human retinal pigment epithelial cells.…”

Section: Glycogen As a Building Block Of Nanostructured Materialsmentioning

confidence: 99%

Glycogen as a Building Block for Advanced Biological Materials

2019

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complex drug delivery systems that are challenging to scale up and therefore have limited clinical and commercial translation. Although numerous nanoparticles have been widely developed and used for various applications, including biomedical applications, [2] there is a growing interest in nontoxic and degradable alternatives to first-generation synthetic nanomaterials. Ideally the synthesis of such nanoparticles needs to be cost effective, simple, green, reproducible, scalable, and the constitutive building blocks of the nanoparticles should be nontoxic, functional, biodegradable, and available on a large scale. Naturally occurring nanoparticles could be such a valid alternative. These nanoparticles include intracellular structures, such as magnetosomes [3] and glycogen, and extracellular assemblies such as lipoproteins and viruses. [3] Among these, glycogen is the most abundant and versatile biological nanoparticle, which fulfils the requirements for the fabrication of nanostructured biomaterials. Glycogen is nature's prime nanoparticle that exists in most organisms, from bacteria and archaea to humans, [4,5] as a vital component of the cellular energy machinery. It is a highly branched polysaccharide comprising repeating units of glucose connected by linear α-d-(1,4) glycosidic linkages with α-d-(1,6) branching, structured into roughly spherical nanoparticles that have a high water solubility and molecular weight.The use of polysaccharides as components for advanced materials has been an attractive concept for decades. [6,7] A key motive is that polysaccharides, as a natural biomaterial, are endowed with a level of biodegradability and biocompatibility. In addition, they can be easily modified chemically or biochemically to produce functional derivatives, which are important for various therapeutic applications. [8] The structures of polysaccharides vary considerably but span from linear to highly branched polymers with molecular weights ranging from thousands to millions, composed of mono-or disaccharides, or short-chain oligomers bound together by glycosidic linkages. [9] There is a rich history of research on the use of polysaccharides in a range of therapeutic applications including: i) soft tissue engineering, [10,11] where the materials can be designed to mimic the mechanical properties of the extracellular matrix in the tissue; ii) as drug, protein, or gene delivery systems, [7,12,13] where polysaccharides have a large number of reactive groups [14] that allow for tunable properties [12] such as pH-responsiveness; [15] and iii) as nanoprobes for cellular Biological nanoparticles found in living systems possess distinct molecular architectures and diverse functions. Glycogen is a unique biological polysaccharide nanoparticle fabricated by nature through a bottom-up approach. The biocatalytic synthesis of glycogen has evolved over time to form a nanometer-sized dendrimer-like structure (20-150 nm) with a highly branched surface and a dense core. This makes glycogen markedly different from other natur...

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Section: Glycogen As a Building Block Of Nanostructured Materialsmentioning

confidence: 99%

Glycogen as a Building Block for Advanced Biological Materials

2019

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“…RLS spectroscopy is a fast, sensitive, and simple technology for investigating complex formation between biopolymers and guest molecules because the RLS intensity is related to complex volumes (Hu et al., 2009; Huang et al., 2006; Liang et al., 2015; Wu, Lai, et al, 2017; Zhang et al., 2009). As shown in Figure 6, the RLS intensities of the TF‐3‐G/α‐GC mixtures were much higher than that of α‐GC (green curve), and increased with increasing TF‐3‐G concentration.…”

Section: Resultsmentioning

confidence: 99%

Insight into interaction mechanism between theaflavin‐3‐gallate and α‐glucosidase using spectroscopy and molecular docking analysis

Liu

Yin

et al. 2020

J. Food Biochem.

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Diabetes is a metabolic disease characterized by hyperglycemia and includes type 1 and type 2 diabetes (Zhang et al., 2016; Zhu et al., 2016). Type 2 diabetes is the most common form of diabetes, accounting for more than 90% of all cases, and is caused by insulin resistance, insufficient insulin secretion, or both (Wang et al., 2019). α-Glucosidase (α-GC), an important glycoside hydrolase in the digestive system, can hydrolyze the glycosidic bonds of starch and oligosaccharides in food and release glucose. Therefore, inhibiting

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