Complexation of Europium(III) with the Zwitterionic Form of Amino Acids Studied with Ultraviolet—Visible and Time-Resolved Laser-Induced Fluorescence Spectroscopy

Heller, Anne; Rönitz, Olivia; Barkleit, Astrid; Bernhard, Gert; Ackermann, Jörg‐Uwe

doi:10.1366/000370210792081127

Cited by 17 publications

(19 citation statements)

References 44 publications

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“…Transition metals including rare earth metals are able to form a diversity of complexes with organic compounds bearing groups that are Lewis bases. Alanine is not an exception, and a number of its complexes with lanthanides [14][15][16][17][18][19] and actinides [20][21][22] have been reported. Three main types of complexation between amino acids and lanthanides are known ( Figure 2).…”

Section: Resultsmentioning

confidence: 99%

Luminescent probing of the simplest chiral α‐amino acid–alanine in an enantiopure and racemic state

et al. 2017

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Luminescent spectroscopy combined with the technique of luminescent probing with rare earth ions (europium, gadolinium, terbium) and an actinide ion (uranyl) was used to differentiate enantiopure and racemic alanine, the simplest chiral proteinogenic amino acid. Using the achiral luminescent probes, small differences between pure L and DL alanine in the solid state were strongly amplified. Based on the observed electronic transitions of the probes, the position of the triplet level of the coordinated alanine was estimated. Formation of homo- and heterochiral complexes between enantiomers of alanine and the metal ions is discussed as a possible mechanism of chiral self-discrimination.

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Section: Resultsmentioning

confidence: 99%

Luminescent probing of the simplest chiral α‐amino acid–alanine in an enantiopure and racemic state

et al. 2017

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“…Similar results were obtained via Heller et al in complexation of alanine with Europium (III) in water. They also showed that the complex stability constants of the carboxyl group of side chain aspartic and glutamic acid with Europium (III) ion were high [42].…”

Section: Discussionmentioning

confidence: 99%

“…Also, Saffar et al showed that a mutated SmtA protein could absorb cadmium ions [24]. In addition, experiments on ion Europium (III) absorption with the zwitterion form of amino acids by means of UV-Vis spectroscopy were studied by Heller et al [42]. Then ionic absorption of proteins can be studied by theoretical and experimental methods.…”

Section: Discussionmentioning

confidence: 99%

“…Specific functional groups in the molecules can absorb light at characteristic wavelengths in the UV-Vis region. This technique can be used to determine the presence of these groups in the sample with investigating the complexation reaction and sustaining structural information obtained from other methods [41,42]. The UVVis absorption spectra were measured using an Ultrospec 3100 pro Pharmacia UV-Vis spectrophotometer at 25°C and pH = 7 and spectra were recorded between 200 and 300 nm.…”

Section: Uv-vis Absorption Spectroscopymentioning

confidence: 99%

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A theoretical and experimental study of calcium, iron, zinc, cadmium, and sodium ions absorption by aspartame

Mahnam

Raisi²

2017

J Biol Phys

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Aspartame (L-Aspartyl-L-phenylalanine methyl ester) is a sweet dipeptide used in some foods and beverages. Experimental studies show that aspartame causes osteoporosis and some illnesses, which are similar to those of copper and calcium deficiency. This raises the issue that aspartame in food may interact with cations and excrete them from the body. This study aimed to study aspartame interaction with calcium, zinc, iron, sodium, and cadmium ions via molecular dynamics simulation (MD) and spectroscopy. Following a 480-ns molecular dynamics simulation, it became clear that the aspartame is able to sequester Fe , and Zn 2+ ions for a long time. Complexation led to increasing UV-Vis absorption spectra and emission spectra of the complexes. This study suggests a potential risk of cationic absorption of aspartame. This study suggests that purification of cadmium-polluted water by aspartame needs a more general risk assessment.

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“…Because the emission spectrum of the Cm 3þ aqua ion peaks at 593.5 nm, 27,29,[44][45][46] this means a red shift of the curium(III) luminescence wavelength in human urine with pH e5.8. Time-resolved measurements exclusively yielded monoexponential decay curves in urine samples with pH e5.8.…”

Section: Curium(iii) and Europium(iii) Complexation With Modelmentioning

confidence: 99%

Chemical Speciation of Trivalent Actinides and Lanthanides in Biological Fluids: The Dominant in Vitro Binding Form of Curium(III) and Europium(III) in Human Urine

Heller

Barkleit

Bernhard

2010

Chem. Res. Toxicol.

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Radionuclides represent a serious health risk to humans in the case of incorporation. To elucidate the potential of time-resolved laser-induced fluorescence spectroscopy (TRLFS) to determine the dominant radionuclide species in natural biofluids, we investigated the in vitro speciation of curium(III) in human urine samples. Because in speciation studies trivalent lanthanides are often used as analogues for trivalent actinides, we also probed the suitability of this theory by investigating the speciation of europium(III) in human urine. Comparison with reference spectra of both heavy metals in model urine and of their complexes with single organic and inorganic urine constituents then allowed for the determination of the dominant species. Furthermore, the chemical composition of all urine samples was analyzed, and the parameters affecting the speciation of the metals were determined. The pH was found to be the most important parameter because for both, the actinide and the lanthanide, two analogue species were identified in dependence on the pH. In samples with slightly acidic pH a curium(III) and europium(III) citrate complex dominates, respectively, whereas in samples with near-neutral pH a higher complex with phosphate and calcium as the main ligands and the additional participation of citrate and/or carbonate is formed in each case. Comparison with thermodynamic modeling yields some discrepancies, especially at higher pH, which is due to a lack of data for the complex formation of the higher species for both heavy metals. Nevertheless, TRLFS has proven to be a suitable method for the direct determination of the dominant heavy metal species in untreated natural human urine samples.

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Complexation of Europium(III) with the Zwitterionic Form of Amino Acids Studied with Ultraviolet—Visible and Time-Resolved Laser-Induced Fluorescence Spectroscopy

Cited by 17 publications

References 44 publications

Luminescent probing of the simplest chiral α‐amino acid–alanine in an enantiopure and racemic state

Luminescent probing of the simplest chiral α‐amino acid–alanine in an enantiopure and racemic state

A theoretical and experimental study of calcium, iron, zinc, cadmium, and sodium ions absorption by aspartame

Chemical Speciation of Trivalent Actinides and Lanthanides in Biological Fluids: The Dominant in Vitro Binding Form of Curium(III) and Europium(III) in Human Urine

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