Complexation of phosphates by 1,3-bis(3-(2-pyridylureido)propyl)-1,1,3,3-tetramethyldisiloxane

Frański, Rafał; Gierczyk, Błażej; Markiewicz, Grzegorz; Kozik, T. A.

doi:10.1002/rcm.7386

Cited by 1 publication

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“…8,9 Although electrospray ionization mass spectrometry (ESI-MS) has been applied to investigate ODN-small molecule complexes, [10][11][12][13] it suffers from poor sensitivity and a lower m/z limit. 17,18 In addition, ODN-drug complexes are usually stable in the presence of salts such as 100 mM NaCl, salt induced suppression of analyte MS signals however occurs, leading to reduced sensitivity. 17,18 In addition, ODN-drug complexes are usually stable in the presence of salts such as 100 mM NaCl, salt induced suppression of analyte MS signals however occurs, leading to reduced sensitivity.…”

Section: Introductionmentioning

confidence: 99%

Nanomaterial based mass spectrometry of oligodeoxynucleotide–drug complexes

et al. 2015

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Surface-assisted laser desorption/ionization mass spectrometry (SALDI-MS) using HgTe nanostructures as the matrix has been employed for the detection of four 15-base oligodeoxynucleotides (ODNs) that are genes found in acute myeloid leukemia (AML) patients and their complexes with mitoxantrone (MTX), which is a common drug for the treatment of AML patients. The major peaks for the four tested ODNs are at m/z values of 4571, 4586, 4610, and 4635, while they are at m/z values of 5017, 5031, 5055, and 5079 for their corresponding complexes with MTX. The ODN with a m/z value of 4610 is assigned to a normal gene of AML, while the other three are single-base mutant ODNs. This approach allows detection of the tested ODNs at concentrations down to 2 nM, showing their potential for the diagnosis of AML. The dissociation constant values of the four tested ODN-MTX complexes determined by the SALDI-MS approach are similar and at the mM level, which agree with that determined by applying a conventional absorption approach. Relative to the conventional approach, the SALDI-MS approach has advantages of simplicity, rapidity, reproducibility, and use of smaller amounts of ODNs and MTX.

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