Complexes of bovine serum albumin with water-soluble Cu and Co containing cationic meso-tetra(4-N-oxyethylpyridyl)-porphyrins

Abstract

Bovine serum albumin complexes with water-soluble cationic porphyrins, Cu - and Co-meso-tetra(4- N -hydroxyethylpyridyl)porphyrins ( CuT4OEPyP , CoT4OEPyP ), and their 3- N -analogs, meso-tetra(3- N -hydroxyethylpyridyl)porphyrins ( CuT3OEPyP , CoT3OEPyP ), have been investigated. The porphyrin-bovine serum albumin binding was monitored by the absorption in the visible region at 400-460 nm. The stoichiometry of binding and the binding constants of the porphyrins to bovine serum albumin were determined using binding isotherms and Scatchard plots. The K b values obtained for these porphyrin- bovine serum albumin complexes are 1.7 × 105 M −1, 3.2 × 105 M −1, 1.4 × 105 M −1 and 3 × 105 M −1 respectively. Binding constants are sensitive to pH and ionic strength of the solution.

DNA damage and anti-tumor activity induced by Zn, Ag and Co containing meso- tetra(4-N-oxyethylpyridyl)porphyrins in vivo

“…All of them have anti-fungal, anti-bacterial, and anti-viral activity [7,8]. The mechanisms of binding of these porphyrins to DNA molecules and to blood transport proteins in vitro were also studied [9,10]. These compounds were studied by our colleagues in vitro for their cyto-and genotoxicity as potential anticancer drugs [11].…”