Complexing of copper in drinking water samples to enhance recovery of <i>Aeromonas</i> and other bacteria

Versteegh, J. F. M.; Havelaar, Arie H.; Hoekstra, A.C.; Visser, A.

doi:10.1111/j.1365-2672.1989.tb02528.x

Cited by 16 publications

(8 citation statements)

References 6 publications

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“…Many of the bacteria used in past studies to characterize metal resistance were isolated from waters polluted with metals (2,14,39,53) and were rarely aeromonads. Miranda and Castillo (39) found 39.3% of aeromonads from raw drinking water supplies with less than 10 2 coliforms/100 ml to be mercury resistant, 3.6% to be chromium resistant, and 57% to be copper resistant, which is in stark contrast to the findings of this and other studies (8,56).…”

Section: Discussioncontrasting

confidence: 56%

Antimicrobial Susceptibilities of Aeromonas spp. Isolated from Environmental Sources

Huddleston

Zak

Jeter

2006

Appl Environ Microbiol

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Aeromonas spp. are ubiquitous aquatic bacteria that cause serious infections in both poikilothermic and endothermic animals, including humans. Clinical isolates have shown an increasing incidence of antibiotic and antimicrobial drug resistance since the widespread use of antibiotics began. A total of 282 Aeromonas pure cultures were isolated from both urban and rural playa lakes in the vicinity of Lubbock, Texas, and several rivers in West Texas and New Mexico. Of these, at least 104 were subsequently confirmed to be independent isolates. The 104 isolates were identified by Biolog and belonged to 11 different species. The MICs of six metals, one metalloid, five antibiotics, and two antimicrobial drugs were determined. All aeromonads were sensitive to chromate, cobalt, copper, nickel, zinc, cefuroxime, kanamycin, nalidixic acid, ofloxacin, tetracycline, and sulfamethoxazole. Low incidences of trimethoprim resistance, mercury resistance, and arsenite resistance were found. Dual resistances were found in 5 of the 104 Aeromonas isolates. Greater numbers of resistant isolates were obtained from samples taken in March versus July 2002 and from sediment versus water. Plasmids were isolated from selected strains of the arsenite-and mercury-resistant organisms and were transformed into Escherichia coli XL1-Blue MRF. Acquisition of the resistance phenotypes by the new host showed that these resistance genes were carried on the plasmids. Mercury resistance was found to be encoded on a conjugative plasmid. Despite the low incidence of resistant isolates, the six playa lakes and three rivers that were sampled in this study can be considered a reservoir for antimicrobial resistance genes.

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Section: Discussioncontrasting

confidence: 56%

Antimicrobial Susceptibilities of Aeromonas spp. Isolated from Environmental Sources

Huddleston

Zak

Jeter

2006

Appl Environ Microbiol

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“…The effect of copper release into the PBS on the viability of recovered organisms was investigated by the addition of ethylenediaminetetraacetic acid (EDTA, 20 mM) which readily complexes free copper. 23 No significant difference was seen (data not shown) between samples recovered into PBS or PBS with EDTA. Thorough analysis of coupons by episcopic differential interference contrast (EDIC) microscopy revealed no attached organisms after washing.…”

Section: Sample Metal Testingmentioning

confidence: 66%

Potential use of copper surfaces to reduce survival of epidemic meticillin-resistant Staphylococcus aureus in the healthcare environment

Noyce

Michels

Keevil

2006

Journal of Hospital Infection

342

229

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“…The effect of copper release into the PBS on the viability of recovered cells (measured in CFU) was investigated by the addition of 20 mM EDTA, which readily complexes free copper (20). No significant difference was seen in the number of colonies formed (data not shown) between samples recovered into PBS or PBS with EDTA.…”

Section: Methodsmentioning

confidence: 99%

Use of Copper Cast Alloys To Control Escherichia coli O157 Cross-Contamination during Food Processing

Noyce

Michels

Keevil

2006

Appl Environ Microbiol

190

133

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The most notable method of infection from Escherichia coli O157 (E. coli O157) is through contaminated food products, usually ground beef. The objective of this study was to evaluate seven cast copper alloys (61 to 95% Cu) for their ability to reduce the viability of E. coli O157, mixed with or without ground beef juice, and to compare these results to those for stainless steel. E. coli O157 (NCTC 12900) (2 ؋ 10 7 CFU) mixed with extracted beef juice (25%) was inoculated onto coupons of each copper cast alloy or stainless steel and incubated at either 22°C or 4°C for up to 6 h. E. coli O157 viability was determined by plate counts in addition to staining in situ with the respiratory indicator fluorochrome 5-cyano-2,3-ditolyl tetrazolium. Without beef extract, three alloys completely killed the inoculum during the 6-h exposure at 22°C. At 4°C, only the high-copper alloys (>85%) significantly reduced the numbers of O157. With beef juice, only one alloy (95% Cu) completely killed the inoculum at 22°C. For stainless steel, no significant reduction in cell numbers occurred. At 4°C, only alloys C83300 (93% Cu) and C87300 (95% Cu) significantly reduced the numbers of E. coli O157, with 1.5-and 5-log kills, respectively. Reducing the inoculum to 10 3 CFU resulted in a complete kill for all seven cast copper alloys in 20 min or less at 22°C. These results clearly demonstrate the antimicrobial properties of cast copper alloys with regard to E. coli O157, and consequently these alloys have the potential to aid in food safety.

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Complexing of copper in drinking water samples to enhance recovery of Aeromonas and other bacteria

Cited by 16 publications

References 6 publications

Antimicrobial Susceptibilities of Aeromonas spp. Isolated from Environmental Sources

Antimicrobial Susceptibilities of Aeromonas spp. Isolated from Environmental Sources

Potential use of copper surfaces to reduce survival of epidemic meticillin-resistant Staphylococcus aureus in the healthcare environment

Use of Copper Cast Alloys To Control Escherichia coli O157 Cross-Contamination during Food Processing

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