Composite of Chitosan-Collagen-&lt;i&gt;Aloe vera&lt;/i&gt; for Scaffolds Application on Skin Tissue

Isfandiary, Andini; Widiyanti, Prihartini; Hikmawati, Dyah

doi:10.4028/www.scientific.net/jbbbe.32.82

Cited by 14 publications

(8 citation statements)

References 17 publications

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“…Because each scaffold application requires a different mechanical property, different tests are used. A scaffold that is applied to the skin requires a tensile test [35]. The tensile test analysis included three aspects: tensile strength, tensile strain, and tensile stiffness.…”

Section: Mechanical Propertiesmentioning

confidence: 99%

Fibroin and Spidroin Thin Film to Support the Attachment and Spread of Human Dermal Fibroblast: The Potency of Skin Tissue Engineering

Nursatya

Barlian

Judawisastra

et al. 2021

J. Math. Fund. Sci.

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This study aimed to determine the characteristics of scaffolds made of fibroin from Bombyx mori and spidroin from Argiope appensa in supporting the attachment and proliferation of HDF cells on the scaffolds. Thin-film scaffolds were made using the solvent casting technique, where the scaffold is an amalgamation of fibroin, spidroin, PVA, and glycerol. HDF cells were grown on DMEM medium with 10% FBS and 1% antibiotic-antimicotic. Characterization of the scaffolds was performed by using ATR-FTIR, swelling test, contact angle measurement, tensile test, biodegradation, MTT and SEM. The results of the ATR-FTIR analysis showed that the scaffolds contained fibroin, spidroin, PVA, and glycerol. Swelling and contact angle tests showed that all scaffold combinations were hydrophilic. Mechanical properties and in vitro biodegradation tests showed no significant difference among the scaffold combinations. MTT testing showed that all scaffolds could facilitate the attachment of fibroblasts and showed increased viability from day 1, 3, and 5. Scanning electron microscopy showed that the cells in the 70% fibroin and 10% spidroin scaffold had the best cell morphology and the best combination for potential application in skin tissue engineering.

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Section: Mechanical Propertiesmentioning

confidence: 99%

Fibroin and Spidroin Thin Film to Support the Attachment and Spread of Human Dermal Fibroblast: The Potency of Skin Tissue Engineering

Nursatya

Barlian

Judawisastra

et al. 2021

J. Math. Fund. Sci.

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“…Isfandiary et al [247] fabricated collagen-chitosan-AV composite scaffolds by a freeze drying method for healing burned skin tissue. The preparation of scaffolds was carried out by dissolving collagen-chitosan (1:1) in 0.05 M acetic acid; then, consequent variants of AV (0%, 0.1%, 0.15%, 0.2%, and 0.25%) were added into solution.…”

Section: Plant Protein-based Green Scaffolds For Tissue Engineeringmentioning

confidence: 99%

“…The preparation of scaffolds was carried out by dissolving collagen-chitosan (1:1) in 0.05 M acetic acid; then, consequent variants of AV (0%, 0.1%, 0.15%, 0.2%, and 0.25%) were added into solution. Results showed that a composite with 0.2% AV was a strong potential candidate to serve as improved scaffolds for burned skin tissue applications [247].…”

Section: Plant Protein-based Green Scaffolds For Tissue Engineeringmentioning

confidence: 99%

Status of Plant Protein-Based Green Scaffolds for Regenerative Medicine Applications

Jahangirian

Azizi²,

Rafiee-Moghaddam

et al. 2019

Biomolecules

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In recent decades, regenerative medicine has merited substantial attention from scientific and research communities. One of the essential requirements for this new strategy in medicine is the production of biocompatible and biodegradable scaffolds with desirable geometric structures and mechanical properties. Despite such promise, it appears that regenerative medicine is the last field to embrace green, or environmentally-friendly, processes, as many traditional tissue engineering materials employ toxic solvents and polymers that are clearly not environmentally friendly. Scaffolds fabricated from plant proteins (for example, zein, soy protein, and wheat gluten), possess proper mechanical properties, remarkable biocompatibility and aqueous stability which make them appropriate green biomaterials for regenerative medicine applications. The use of plant-derived proteins in regenerative medicine has been especially inspired by green medicine, which is the use of environmentally friendly materials in medicine. In the current review paper, the literature is reviewed and summarized for the applicability of plant proteins as biopolymer materials for several green regenerative medicine and tissue engineering applications.

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“…Experimental results, using cell viability assay and cell attachment observation, showed that the nano-fibrous membranes support 3T3 cell viability and could be a potential candidate for GTR therapy. Isfandiary et al [26] sought the procedures to synthesize composite scaffolds for burns utilizing collagen-chitosan-AV to find the best concentration of AV as scaffolds application. The synthesis of scaffolds was performed with collagen constituent composition as follow: collagen-chitosan (1:1) was dissolved in 0.05M acetic acid, then subsequent variations of AV, namely 0% AV, 0.1% AV, 0.15% AV, 0.2% AV, and 0.25% AV were added into collagen-chitosan solution, and freeze dry method was applied.…”

Section: Aloe Vera Gel Scaffolds With Semi-synthetic or Natural (Curcmentioning

confidence: 99%

Nutraceutical Aloe Vera Gel Scaffolds for Supporting a Healthy Muscle Movement

Yagi

Hasegawa

Mikami³

et al. 2018

Journal of Gastroenterology and Hepatology Research

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The efficacy for pain and stiffness associated with arthralgia of the knee and hip was evaluated based on locomotive syndrome risk test. Furthermore, w-3polyunsaturated fatty acids (EPA and DHA) and L-Arginine with AVJ revealed a possible dietary efficacy for Parkinson's disease (PD) patients at stage 3. Prophylactic possible efficacies of AVJ as bio-functional scaffolds are fully expected for increasing collagen synthesis in knee and/or hip joint healing and for mitigation of risk factors of PD patients with diabetes.

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Composite of Chitosan-Collagen-<i>Aloe vera</i> for Scaffolds Application on Skin Tissue

Cited by 14 publications

References 17 publications

Fibroin and Spidroin Thin Film to Support the Attachment and Spread of Human Dermal Fibroblast: The Potency of Skin Tissue Engineering

Fibroin and Spidroin Thin Film to Support the Attachment and Spread of Human Dermal Fibroblast: The Potency of Skin Tissue Engineering

Status of Plant Protein-Based Green Scaffolds for Regenerative Medicine Applications

Nutraceutical Aloe Vera Gel Scaffolds for Supporting a Healthy Muscle Movement

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