In case of burns, scaffolds serve as cover for burns and facilitate cells regeneration. Accordingly, this study seeks procedures to synthesize composite scaffolds for burns utilizing collagen-chitosan-Aloe vera to find the best concentration of Aloe vera as scaffolds application. The synthesis of scaffolds was performed with collagen constituent composition as follow: chitosan (1:1) was dissolved in 0.05M Acetic Acid, then subsequent variations of Aloe vera (AV), namely 0% AV; 0.1% AV; 0.15% AV; 0.2% AV; and 0.25% AV were added into collagen-chitosan solution, and freeze dry method was applied. Sample characterization was done by FTIR, tensile strength test, SEM test, cytotoxicity assay and degradation test. Typical absorption bands of collagen in the FTIR test was obtained at 1645.5 cm-1 (C = O stretch), chitosan at 1540.28 cm-1 (NH stretch) and Aloe vera at 3474.41 cm-1 (NH stretch). Tensile test data showed the highest number of tensile strength from the 0.1% AV sample at 0.017 MPa. The SEM test revealed a pore size of <50 μm. During cytotoxicity assay, the entire sample is not toxic. Degradation test demonstrated that overall sample was not exhausted within two weeks. In conclusion, sample with 0.2% AV was potential as scaffolds for burns skin tissue.

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Head trauma was the third cause of deaths that have a high rank that can make serious head injury for 25.5%-54.9%. This study has been conducted by making a replacement layer of the brain (dura) to overcome the impact of dural defect by utilizing waste fish scales red snapper (Lutjanus sp.). Synthesis brain membranes lining processed by casting method with each various concentrations of chitosan coating of 1%, 1.5%, and 2% then dried using vacuum dry. The samples then were characterized by tensile test, FTIR, SEM and MTT Assay. FTIR test results showed that red snipperscales can produce collagen powder at amide A group with stretching of –NH functional group, amide B group has stretching of CH2 assymetry, amide I area, amide II and amide III area which show –NH bonding. Tensile test results showed that the combination between PLGA-Collagen Chitosan Coating 2% produced the highest tensile strength is 4.8 MPa which meet the standards of human duramater strength. MTT Assay results showed that the dural membrane produced no toxic seen from living cells reached 98.32%. Poly - Glycolyc Lactic - Co Acid (PLGA) - collagen coating chitosan based on red snapper fish scales (Lutjanus sp.) composites has potency as duramater artificial candidate due to the chemistry, biological and physical characteristics.

Head trauma is the third cause of deaths with a high rank, causing severe head injury for 25.5%-54.9%. This study has been conducted by making a replacement layer of the brain (dura) to overcome the dural defect's impact by utilizing waste fish scales red snapper (Lutjanus Sp.). Synthesis brain membranes lining processed by casting method with each various chitosan coating concentrations of 1%, 1.5%, and 2%, then dried using vacuum dry. The samples were then characterized by tensile tests, FTIR, SEM, and MTT Assay. FTIR test results showed that red snipper scales could produce collagen powder at amide A group with stretching of –NH functional group, amide B group, has stretching of CH2 asymmetry, amide I area, amide II, and amide III area, which show –NH bonding. Tensile test results showed that the combination of PLGA-Collagen Chitosan Coating 2% produced the highest tensile strength is 4.8 MPa, which met human dura mater strength standards. MTT Assay results showed that the dural membrane produced no toxic seen from living cells reached 98.32%. Poly - Glycolic Lactic - Co Acid (PLGA) - collagen coating chitosan-based on red snapper fish scales (Lutjanus Sp.) composites has potency dura mater, artificial candidate, due to the chemistry, biological, and physical characteristics.