Composition and Diversity of Gut Bacterial Community in Different Life Stages of a Leaf Beetle Gastrolina depressa

Ma, Meiqi; Chen, Xiaotong; Li, Siqun; Luo, Jing; Han, Richou; Xu, Letian

doi:10.1007/s00248-022-02054-0

Cited by 18 publications

(9 citation statements)

References 77 publications

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“…The high-quality sequences imported into the Quantitative Insights Into Microbial Ecology version 2 (QIIME2) pipeline (http://qiime2.org, accessed on 18 January 2022) were processed to generate amplicon sequence variants (ASVs) via DADA2 (http://github.com/benjjneb/data2, accessed on 18 January 2022) based on 100% sequence similarity [48][49][50]. The taxonomic classification of ASVs was performed using QIIME2 through assigning against the SILVA databases, along with blasting the NCBI database manually with BLAST searches [51,52].…”

Section: Dna Extraction and Iiiumina High-throughput Sequencing Of Ba...mentioning

confidence: 99%

A Study on Symbiotic Systems of Cicadas Provides New Insights into Distribution of Microbial Symbionts and Improves Fluorescence In Situ Hybridization Technique

Huang

Zhou

Zhang

et al. 2023

IJMS

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Nutritional symbionts of sap-sucking auchenorrhynchan insects of Hemiptera are usually confined to the bacteriomes and/or fat bodies. Knowledge is limited about the distribution of microbial symbionts in other organs. We investigated the distribution of obligate symbionts in the salivary glands, gut tissues, reproductive organs, bacteriomes, and fat bodies of two cicada species, Karenia caelatata and Tanna sp., using integrated methods, including a modified fluorescence in situ hybridization (FISH) technique, which can greatly enhance the FISH signal intensity of related symbionts. We revealed that Candidatus Sulcia muelleri (Sulcia) and a yeast-like fungal symbiont (YLS) were harbored in the bacteriomes and fat bodies, respectively. Both of Sulcia and YLS can be transmitted to the offspring via ovaries, forming a “symbiont ball” in each egg. Neither Sulcia nor YLS were harbored in the salivary glands, gut tissues and testes. Phylogenetic trees of both Sulcia and cicadas confirm that K. caelatata is a member of the tribe Dundubiini, and the tribe Leptopsaltriini that comprises Ta. sp. is not monophyletic. YLS of K. caelatata is embedded inside the lineage of YLS of Dundubiini, whereas YLS of Ta. sp. is closely related to the clade comprising both cicada-parasitizing fungi Ophiocordyceps and YLS of Mogannia conica and Meimuna mongolica, suggesting an evolutionary replacement of YLS in Ta. sp. from an Ophiocordyceps fungus to another Ophiocordyceps fungus. Our results provide new insights into the symbiosis between Cicadidae and related symbionts. Modification through the addition of helpers and heat shock greatly enhanced the FISH signal intensity of YLS, which may provide guidelines for enhancement of the hybridization signal intensity of other symbiont(s) in the FISH experiments.

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Section: Dna Extraction and Iiiumina High-throughput Sequencing Of Ba...mentioning

confidence: 99%

A Study on Symbiotic Systems of Cicadas Provides New Insights into Distribution of Microbial Symbionts and Improves Fluorescence In Situ Hybridization Technique

Huang

Zhou

Zhang

et al. 2023

IJMS

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“…Previous studies have indicated that some gut commensal bacteria can be vertically transmitted by attaching onto the surface of mosquito eggs ( 41 , 42 ) and are released into aquatic habitats and then transstadially transmitted to the gut of emerging adult offspring ( 43 , 44 ). We therefore washed off the attached bacterial cells by vortexing the eggs that were collected from field-caught A. albopictus mosquitoes.…”

Section: Resultsmentioning

confidence: 99%

A naturally isolated symbiotic bacterium suppresses flavivirus transmission byAedesmosquitoes

Zhang,

Wang,

Shi

et al. 2024

Science

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The commensal microbiota of the mosquito gut plays a complex role in determining the vector competence for arboviruses. In this study, we identified a bacterium from the gut of field Aedes albopictus mosquitoes named Rosenbergiella sp. YN46 ( Rosenbergiella_ YN46) that rendered mosquitoes refractory to infection with dengue and Zika viruses. Inoculation of 1.6 × 10 3 colony forming units (CFUs) of Rosenbergiella_ YN46 into A. albopictus mosquitoes effectively prevents viral infection. Mechanistically, this bacterium secretes glucose dehydrogenase ( Ry GDH), which acidifies the gut lumen of fed mosquitoes, causing irreversible conformational changes in the flavivirus envelope protein that prevent viral entry into cells. In semifield conditions, Rosenbergiella_ YN46 exhibits effective transstadial transmission in field mosquitoes, which blocks transmission of dengue virus by newly emerged adult mosquitoes. The prevalence of Rosenbergiella_ YN46 is greater in mosquitoes from low-dengue areas (52.9 to ~91.7%) than in those from dengue-endemic regions (0 to ~6.7%). Rosenbergiella_ YN46 may offer an effective and safe lead for flavivirus biocontrol.

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“…S2). Proteobacteria are a dominant environmental bacterial community, including in the insect gut ( 19 , 20 ), and their proportion in the diet of rhinoceros beetles is higher than their proportion in the beetles’ gut tract ( Fig. 1A ; Fig.…”

Section: Resultsmentioning

confidence: 99%

Trypoxylus dichotomus Gut Bacteria Provides an Effective System for Bamboo Lignocellulose Degradation

Huang¹,

Weng²,

Zhang³

et al. 2022

Microbiol Spectr

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Composition and Diversity of Gut Bacterial Community in Different Life Stages of a Leaf Beetle Gastrolina depressa

Cited by 18 publications

References 77 publications

A Study on Symbiotic Systems of Cicadas Provides New Insights into Distribution of Microbial Symbionts and Improves Fluorescence In Situ Hybridization Technique

A Study on Symbiotic Systems of Cicadas Provides New Insights into Distribution of Microbial Symbionts and Improves Fluorescence In Situ Hybridization Technique

A naturally isolated symbiotic bacterium suppresses flavivirus transmission byAedesmosquitoes

Trypoxylus dichotomus Gut Bacteria Provides an Effective System for Bamboo Lignocellulose Degradation

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