Composition and diversity of intestinal coliform flora influence bacterial translocation in rats after hemorrhagic stress

Katouli, Mohammad; Bark, T.; Ljungqvist, Olle; Svenberg, T.; Möllby, R.

doi:10.1128/iai.62.11.4768-4774.1994

Cited by 31 publications

(11 citation statements)

References 38 publications

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“…Simpson's index of diversity (1/D), which considers both richness and evenness, was used to measure diversity. This index has been used previously for microbial communities (Katouli et al, 1994;Zhou et al, 2002). It has been suggested that values for 1/D below about 50 indicate typical dominance profiles; therefore, all three libraries showed dominance.…”

Section: Phylogenetic Analysis and Statistical Indicators Of Clone LImentioning

confidence: 99%

Intestinal TM7 bacterial phylogenies in active inflammatory bowel disease

Kuehbacher

Rehman

Lepage

et al. 2008

Journal of Medical Microbiology

168

127

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TM7 is a recently described subgroup of Gram-positive uncultivable bacteria originally found in natural environmental habitats. An association of the TM7 bacterial division with the inflammatory pathogenesis of periodontitis has been previously shown. This study investigated TM7 phylogenies in patients with inflammatory bowel diseases (IBDs). The mucosal microbiota of patients with active Crohn's disease (CD; n542) and ulcerative colitis (UC; n531) was compared with that of controls (n533). TM7 consortia were examined using molecular techniques based on 16S rRNA genes, including clone libraries, sequencing and in situ hybridization. TM7 molecular signatures could be cloned from mucosal samples of both IBD patients and controls, but the composition of the clone libraries differed significantly. Taxonomic analysis of the sequences revealed a higher diversity of TM7 phylotypes in CD (23 different phylotypes) than in UC (10) and non-IBD controls (12). All clone libraries showed a high number of novel sequences (21 for controls, 34 for CD and 29 for UC). A highly atypical base substitution for bacterial 16S rRNA genes associated with antibiotic resistance was detected in almost all sequences from CD (97.3 %) and UC (100 %) patients compared to only 65.1 % in the controls. TM7 bacteria might play an important role in IBD similar to that previously described in oral inflammation. The alterations of TM7 bacteria and the genetically determined antibiotic resistance of TM7 species in IBD could be a relevant part of a more general alteration of bacterial microbiota in IBD as recently found, e.g. as a promoter of inflammation at early stages of disease.

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Section: Phylogenetic Analysis and Statistical Indicators Of Clone LImentioning

confidence: 99%

Intestinal TM7 bacterial phylogenies in active inflammatory bowel disease

Kuehbacher

Rehman

Lepage

et al. 2008

Journal of Medical Microbiology

168

127

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“…The translocation of enteric bacteria depends on disturbance of the intestinal microecology, with subsequent bacterial overgrowth, impaired host immunity or physical disruption of the gut mucosal barrier (Katouli et al . ). GF mice develop without intestinal microbiota, so bacterial translocation will not be observed in GF mice.…”

Section: Resultsmentioning

confidence: 97%

Intestinal dysbacteriosis contributes to decreased intestinal mucosal barrier function and increased bacterial translocation

Wang

Zhang

Zuo

et al. 2013

Lett Appl Microbiol

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Significance and Impact of the Study: Colistin has been reported to be effective in selective digestive decontamination (SDD), which is an infection prevention measure used in the treatment of certain patients in intensive care. We are the first to report that colistin-induced intestinal dysbacteriosis can injure intestinal mucosal barrier function and increase bacterial translocation, whereas a high dose of colistin does not damage the intestinal mucosal barrier in germ-free (GF) mice raised in a GF environment. These results may indicate that prolonged use of a high dose of a SDD medication should be carefully considered. AbstractThe purpose of this study was to determine the effect of colistin-induced intestinal dysbacteriosis on intestinal mucosal barrier function and bacterial translocation in a mouse model. Colistin or saline was administered orally for 7 days, and populations of viable organisms from the caecal mucosa and its content, the ileal segments, the mesenteric lymph nodes (MLNs) and the internal organs were prepared for examination. In the intestinal dysbacteriosis model, intestinal barrier dysfunction was observed and associated with increased bacterial translocation to extraintestinal sites. The extent of bacterial translocation to the MLNs and internal organs in the colistin group was significantly higher than in the saline group. Colistin-induced intestinal dysbacteriosis was shown to injure the intestinal mucosa barrier function and increase bacterial dislocation.

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“…Recently, it has been evidenced that also the strain of bacteria is important for its capacity to translocate. For instance, special strains of E.coli have been reported to be more efficient translocators as compared to others (146,164,198). One explanation could be that several strains of E. coli show better adherence and attachment to the mucus-epithelium layer than non-pathogenic bacteria (23).…”

Section: Intestinal Microfloramentioning

confidence: 99%

Bacterial Translocation

Wiest

2005

Bioscience Microflora

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This lecture first highlights the methods available to detect bacterial translocation (BT) as well as shortcomings related to those. Secondly, mechanisms involved in the process of BT with special focus on intestinal flora, gut barrier dysfunction and the role of immune defence mechanisms are discussed. Thirdly, the potentially huge clinical relevance of BT is reviewed. BT is the key to our understanding of spontaneous bacterial peritonitis in liver cirrhosis, the multiorgan failure syndrom induced by hemorrhagic shock, burns or sepsis and is involved in a multitude of diverse gastrointestinal lesions. Therefore, BT may represent a common and so far inadequately recognized trigger for a multitude of complications known for long in various clinical entities. Finally, the focus will be to discuss advances in therapeutic approaches in preventing BT and associated potential complications. There is much evidence linking diet with the maintenance of intestinal integrity and multiple enteral manoveures have been tested. Particularly promising is the avenue of using probiotics known to exert many effects and to protect the gut from BT. Various other therapeutic approaches include binding/neutralizing of endotoxins, hyperbaric oxygen, prostaglandins, antioxidans etc. A more general attempt to reduce the presumed risk of BT is selective gut decontamination (SGD). In summary, the concept of BT contributing to morbidity is of extraordinary importance and its detailed investigation will eventually open an avenue for preventive measures against infectious complications.

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Composition and diversity of intestinal coliform flora influence bacterial translocation in rats after hemorrhagic stress

Cited by 31 publications

References 38 publications

Intestinal TM7 bacterial phylogenies in active inflammatory bowel disease

Intestinal TM7 bacterial phylogenies in active inflammatory bowel disease

Intestinal dysbacteriosis contributes to decreased intestinal mucosal barrier function and increased bacterial translocation

Bacterial Translocation

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