Composition and diversity of prokaryotes at an iron ore post‐mining site revealed the natural resilience 10 years after mining exploitation

Abstract

Prokaryotes play crucial roles in the rehabilitation process to restore the ecological integrity of disturbed areas. This work reports on the profiles of N-fixing microorganisms and Actinobacteria, from DGGE, the prokaryotic composition from nextgeneration sequencing (NGS) and physicochemical soil characteristics. We compared a deactivated mining site where mining ended about 10 years ago, when the revegetation process was begun (RV), and a reference site, with natural vegetation (NT), both located at Retiro das Almas Mine, in the Quadrilátero Ferrífero region, Minas Gerais, Brazil. In both sites, the most abundant archaeal and bacterial groups included Euryarchaeota, Thaumarchaeota, Proteobacteria, Actinobacteria, Acidobacteria, and Verrucomicrobia and revealed differences in their ecological metrics and distribution.Proteobacteria and Actinobacteria were most abundant in RV sites, while Acidobacteria and Verrucomicrobia were most abundant in NT sites. Less abundant phyla, such as Bathyarchaeota (Archaea) and GAL 15 (Bacteria) were found only in NT, while Gracilibacteria, Ignavibacteriae BJ-169, and BRC1 were only found in RV. The majority of identified bacterial genera were shared by RV and NT. Soil P, pH, and particle density were most significant (p < 0.05) in RV, while Fe, Ca, organic matter, potential acidity and dispersed clays, were most significant (p < 0.05) in NT, showing differences in soil characteristics, which led the prokaryotic composition in these sites. DGGE profiles of N-fixing microorganisms revealed N-fixing predominance in both sites, although after 10 years prokaryotes diversity increased in RV site. Our results revealed that prokaryotic structure and composition are indicative of RV soil resilience.

Insight into the Characteristics of Soil Microbial Diversity during the Ecological Restoration of Mines: A Case Study in Dabaoshan Mining Area, China

“…In addition, the ecological restoration of the open pit coal mine in Inner Mongolia steppe area promoted the rapid improvement of plant community and soil physicochemical properties, and made the height, coverage, and aboveground biomass of plant community higher than or close to the original steppe [22]. The restoration of vegetation in the ecological restoration of the mining area improves the physicochemical properties of soil, improves the diversity of microbial community, and reduces the content of heavy metals in soil [20,23,24].…”