Composition of Artificial Tear Solution Affects<i>In Vitro Pseudomonas aeruginosa</i>Biofilm Formation on Silicone Hydrogel Lens

Yadav, Mukesh; Chuck, Roy S.; Park, Keun

doi:10.1089/jop.2012.0078

Cited by 7 publications

(2 citation statements)

References 30 publications

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“…Not only must they provide moisture to the injury, but also gel formulation must maintain drug stability during shelf time as well as an appropriate release during their administration. Several works have demonstrated that during hydrogel formulation, different excipients have variations in drug release . With regards to chemical stability, SOSs and chlorine can be exhausted into hydrogel because their high reactivity ; this idea is supported by our results because chlorine containing and SOSs were less effective against biofilm formation and elimination than DADS‐M or biguanide.…”

Section: Discussionsupporting

confidence: 77%

In vitro microbicidal, anti‐biofilm and cytotoxic effects of different commercial antiseptics

Ortega‐Peña

Hidalgo-González

Robson

et al. 2016

International Wound Journal

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Topical antiseptics are widely used for wound treatment, with the goal of disrupting biofilm capacity. We analysed the effectiveness of a variety of antiseptics to inhibit various stages of biofilm formation and to remove biofilms in vitro as well as the agents' cytotoxic effects on fibroblasts. We found that the chlorine-releasing agents exhibited immediate anti-biofilm effects in the short term, with lesser cytotoxicity than agents prepared from more stable compounds, such as biguanide or modified diallyl disulfide-oxide, which, conversely, have better long-term effectiveness. Among the examined organisms, Gram-positive bacteria and Candida albicans were the most sensitive to the antiseptics, whereas Pseudomonas aeruginosa and Acinetobacter baumannii were relatively resistant to them. Formulations whose mechanisms of action involve the release of chemically active chlorine were more effective when administered in solution than the gel form, likely because of the stability of the active ingredients during or after preparation of the formula. Interestingly, hypochlorous acid and some superoxidation solutions were effective in preventing biofilm formation within a short time period and showed virtually no toxicity. Our study indicates that most antiseptics remain effective long enough to prevent biofilm formation; thus, even brief application of an antiseptic agent during initial wound treatment can lead to better wound management outcomes.

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Section: Discussionsupporting

confidence: 77%

In vitro microbicidal, anti‐biofilm and cytotoxic effects of different commercial antiseptics

Ortega‐Peña

Hidalgo-González

Robson

et al. 2016

International Wound Journal

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“…Costagliola et al (45) found that staphylococci and streptococci were obtained low-molecular weight sugars that could be used as nutrients by producing hyaluronidase in the presence of HA. Some bacteria such as Pseudomonas aeruginosa did not have the ability to produce hyaluronidase, thus limiting the benefit of sodium hyaluronate to them (46,47). However, these results are not based on a complete microbiome, and therefore cannot explain whether this phenomenon occurs in the ocular surface microbiome.…”

Section: Discussionmentioning

confidence: 99%

Effects of Sodium Hyaluronate Eye Drops With or Without Preservatives on Ocular Surface Bacterial Microbiota

et al. 2022

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PurposeThis study aimed to determine the composition and diversity of bacterial communities on the ocular surface before and after the intervention with sodium hyaluronate eye drops (with or without preservatives) using 16S rRNA gene amplicon sequencing.MethodsSixteen healthy adults were randomly divided into two groups and treated with sodium hyaluronate eye drops with or without preservatives for 2 weeks. The individuals used the same artificial tears in both eyes. The microbial samples from the conjunctival sac of each participant were collected at baseline and 2 weeks after intervention. The diversity and taxonomic differences among different groups before and after intervention were compared by sequencing the V3–V4 region of the 16S rRNA gene.ResultsThe similarity in the binocular microbial community was high in 1 of the 16 volunteers (Bray-Curtis dissimilarity score < 0.3). At the genus level, 11 bacteria were detected in all samples with an average relative abundance of more than 1%. The bacterial community changed significantly after the use of sodium hyaluronate eye drops (with or without preservatives), whether within individuals or between individuals in different groups (P < 0.05, PERMANOVA). Different dosage forms of sodium hyaluronate eye drops significantly decreased the relative abundance of Flavobacterium caeni and Deinococcus antarcticus, respectively (P < 0.05).ConclusionsHealthy people had a rich diversity of the bacterial microbiota on the ocular surface, but the bacterial communities between the eyes were not completely similar. Irrespective of containing benzalkonium chloride (BAC), sodium hyaluronate eye drops can change the bacterial community on the ocular surface.

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Biofilm formation: A well-played game in bacterial pathogenesis

Thite¹,

Bharathi²,

Srinandan³

2023

Understanding Microbial Biofilms

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Composition of Artificial Tear Solution AffectsIn Vitro Pseudomonas aeruginosaBiofilm Formation on Silicone Hydrogel Lens

Abstract: P. aeruginosa biofilm formation on silicone hydrogel contact lens was affected by the composition of artificial tears. A higher concentration of HA and addition of benzalkonium chloride or dextran in artificial tears were helpful to prevent P. aeruginosa biofilm formation.

Cited by 7 publications

References 30 publications

In vitro microbicidal, anti‐biofilm and cytotoxic effects of different commercial antiseptics

In vitro microbicidal, anti‐biofilm and cytotoxic effects of different commercial antiseptics

Effects of Sodium Hyaluronate Eye Drops With or Without Preservatives on Ocular Surface Bacterial Microbiota

Biofilm formation: A well-played game in bacterial pathogenesis

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