Composition of commercial media used for human embryo culture

Morbeck, Dean E.; Krisher, Rebecca L.; Herrick, Jason R.; Baumann, N; Matern, Dietrich; Moyer, Thomas P.

doi:10.1016/j.fertnstert.2014.05.043

Cited by 152 publications

(128 citation statements)

References 69 publications

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“…Table 1. According to these results, there is no significant differences between the added L-CySH concentration and that found by our electrochemical sensor in synthetic cell culture media, with recoveries ranging between 95 % and 101 %, thereby there is no significant matrix effect and therefore L-CySH determination in the presence of a high amount of amino acids [58], could be feasible using CoPc-SPEs in real cell culture media. Table 1.…”

Section: Analytical Figures Of Meritsupporting

confidence: 47%

l -Cysteine determination in embryo cell culture media using Co (II)-phthalocyanine modified disposable screen-printed electrodes

Hernández-Ibáñez

Sanjuán

Montiel

et al. 2016

Journal of Electroanalytical Chemistry

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Thiol-containing compounds such as L-cysteine have been demonstrated to play an important role in metabolism and cellular growth, acting as powerful antioxidants.Consequently, their analytical determination in biological media has received a considerable amount of attention. In this work, an electrochemical sensor for the accurate electroanalytical determination of L-cysteine is proposed, based upon a Co(II)-phthalocyanine nanoparticle bulk modified disposable screen-printed graphite electrode (CoPc-SPE). This CoPc mediator has previously been introduced through its incorporation within the bulk of the ink formulation, avoiding the drop-casting method, which has been shown to give a low reproducibility. Cyclic (CV) and Square Wave

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Section: Analytical Figures Of Meritsupporting

confidence: 47%

l -Cysteine determination in embryo cell culture media using Co (II)-phthalocyanine modified disposable screen-printed electrodes

Hernández-Ibáñez

Sanjuán

Montiel

et al. 2016

Journal of Electroanalytical Chemistry

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“…Earlier efforts to culture human embryos to the blastocyst stage in less sophisticated media resulted in acceptable blastocyst formation rates but very low pregnancy rates [5][6][7][8], indicating that human embryos are capable of developing in diverse culture media, not all of which appear to generate viable embryos with a capacity to implant and result in a pregnancy. The adaptability of human embryos to varying culture conditions has prompted investigation and discussions regarding possible downstream long-term effects of single versus sequential culture on pregnancy outcome and offspring phenotype [43][44][45][46][47].…”

Section: Discussionmentioning

confidence: 99%

Blastocyst culture using single versus sequential media in clinical IVF: a systematic review and meta-analysis of randomized controlled trials

Sfontouris

Martins

Nastri

et al. 2016

J Assist Reprod Genet

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Purpose The purpose of this study was to undertake a review of the available evidence comparing the use of a single medium versus sequential media for embryo culture to the blastocyst stage in clinical IVF. Methods We searched the Cochrane Central, PubMed, Scopus, ClinicalTrials.gov, Current Controlled Trials and WHO International Clinical Trials Registry Platform to identify randomized controlled trials comparing single versus sequential media for blastocyst culture and ongoing pregnancy rate. Included studies randomized either oocytes/zygotes or women. Eligible oocyte/zygote studies were analyzed to assess the risk difference (RD) and 95 % confidence intervals (CI) between the two media systems; eligible woman-based studies were analyzed to assess the risk ratio (RR) and 95 % CI for clinical pregnancy rate. Results No differences were observed between single and sequential media for either ongoing pregnancy per randomized woman (relative risk (RR) = 0.9, 95 % CI = 0.7 to 1.3, two studies including 246 women, I 2 = 0 %) or clinical pregnancy per randomized woman (RR = 1.0, 95 % CI = 0.7 to 1.4, one study including 100 women); or miscarriage per clinical pregnancy: RR = 1.3, 95 % CI = 0.4 to 4.3, two studies including 246 participants, I 2 = 0 %). Single media use was associated with an increase blastocyst formation per randomized oocyte/ zygote (relative distribution (RD) = +0.06, 95 % CI = +0.01 to Declaration of Authors' Roles Conception and design (CR, LR, WPM, IAS, and NR-F); search strategy (WPM, IAS); data extraction (WPM, PAN, IGRV, CON), analysis, and interpretation (all authors); writing the article (all authors). All authors had full access to all the data in the study and approved the final manuscript.Capsule In conclusion, there is insufficient evidence to recommend either sequential or single-step media as being superior for the culture of embryos to days 5/6 blastocyst stage. +0.12, ten studies including 7455 oocytes/zygotes, I 2 = 83 %) but not top/high blastocyst formation (RD = +0.05, 95 % CI = −0.01 to +0.11, five studies including 3879 oocytes/zygotes, I 2 = 93 %). The overall quality of the evidence was very low for all these four outcomes. Electronic supplementary materialConclusions Although using a single medium for extended culture has some practical advantages and blastocyst formation rates appear to be higher, there is insufficient evidence to recommend either sequential or single-step media as being superior for the culture of embryos to days 5/6. Future studies comparing these two media systems in well-designed trials should be performed.

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“…Therefore, glucose is often added to the culture medium for use with zygotes at the low concentration of 0.2‐0.5 mmol L −1 124. The detailed composition of the embryo culture media that is used clinically has not been published to date for commercial reasons; however, according to the analysis by Morbeck et al.,125 it resembles either the G1/G2 medium that was developed by Gardner and Lane126 or the KSOM AA medium that was developed by Biggers et al 127. More information on the culture media for assisted reproductive technology is available elsewhere 124…”

Section: History Of Cell Culture Mediamentioning

confidence: 99%

Animal‐cell culture media: History, characteristics, and current issues

Yao

Asayama

2017

Reprod Medicine & Biology

394

278

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BackgroundCell culture technology has spread prolifically within a century, a variety of culture media has been designed. This review goes through the history, characteristics and current issues of animal‐cell culture media.MethodsA literature search was performed on PubMed and Google Scholar between 1880 and May 2016 using appropriate keywords.ResultsAt the dawn of cell culture technology, the major components of media were naturally derived products such as serum. The field then gradually shifted to the use of chemical‐based synthetic media because naturally derived ingredients have their disadvantages such as large batch‐to‐batch variation. Today, industrially important cells can be cultured in synthetic media. Nevertheless, the combinations and concentrations of the components in these media remain to be optimized. In addition, serum‐containing media are still in general use in the field of basic research. In the fields of assisted reproductive technologies and regenerative medicine, some of the medium components are naturally derived in nearly all instances.ConclusionsFurther improvements of culture media are desirable, which will certainly contribute to a reduction in the experimental variation, enhance productivity among biopharmaceuticals, improve treatment outcomes of assisted reproductive technologies, and facilitate implementation and popularization of regenerative medicine.

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Composition of commercial media used for human embryo culture

Cited by 152 publications

References 69 publications

l -Cysteine determination in embryo cell culture media using Co (II)-phthalocyanine modified disposable screen-printed electrodes

l -Cysteine determination in embryo cell culture media using Co (II)-phthalocyanine modified disposable screen-printed electrodes

Blastocyst culture using single versus sequential media in clinical IVF: a systematic review and meta-analysis of randomized controlled trials

Animal‐cell culture media: History, characteristics, and current issues

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