Composition of ether‐linked sub‐classes of glycerophospholipids in clones with a different metastatic potential isolated from a murine fibrosarcoma line (T3 cells)

Abstract

An increase of ether-linked sub-classes of choline and ethanolamine glycerophospholipids has been shown in different types of tumor cells, and correlated with some of their specific biological parameters. In the present study, we examined the composition of ether-linked lipids in a series of clones with a different lung-colonizing potential isolated in our laboratory from a highly metastatic fibrosarcoma line (T3 cells). We found good correlation between the metastatic potential of T3 isolates and increased proportions of both alkylacyl and alkenylacyl subclasses in choline glycerophospholipids (CGP). Moreover, propagation of a weakly metastatic T3 clone in tissue culture led to the emergence of a sub-clone which expressed high metastatic potential together with a high level of alkylacyl and alkenylacyl CGP. No differences were found in the alkylacyl and alkenylacyl-ethanolamine glycerophospholipids (EGP) between the strongly and weakly metastatic T3 clones. We discuss the accumulation of alkylacyl and alkenylacyl CGP in metastatic cells for its possible role in metastatic diffusion by generation of platelet-activating factor (PAF).

Platelet‐activating factor and liver metastasis of colorectal cancer

“…Thus, PAF acts on the growth of various human tumor cell lines, 5,6 increases adhesiveness of tumor cells to vascular endothelia, 7 enhances oncogene expression 8 and can contribute to tumor development by enhancing cell motility and by stimulating the angiogenic response. 6,9 Data report the accumulation of AAGPC in metastatic cells 10 and the role of PAF in metastatic diffusion in mice. 11 The contribution of PAF in human solid tumors is poorly documented.…”

Lipid Markers of Breast Tissue for the Diagnosis of Regional Metastatic Lesion

“…Essential phospholipids are known as lipid markers of neoplastic tissue damage 22 . A higher content of phosphotidylcholides and phosphotidylethanolamines with an ether bond was recorded for the cell lines with a high metastatic potential compared to the cell lines with a low metastatic potential 23 .…”

Effect of radiotherapy and chemotherapy on composition of tumor membrane phospholipids

“…Although the function of plasmalogens and alkylacyl glycerophospholipids remain obscure, diradylglycerols, metabolites of plasmalogens and alkylacyl glycerophospholipids, have been shown to activate protein kinase C (27), and PlaCho is known to be a precursor of plateletactivating factor (28). Elevated levels of PlaCho have been correlated with metastatic potential in rat mammary carcinomas (29) and in clones of T3 cells of different metastatic potential (30). It is possible therefore that the reduction in PlaCho following cyclophosphamide therapy in this study is related to a loss in the metastatic potential of the tumor.…”