2004
DOI: 10.1021/jf035023m
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Composition of Grain and Forage from Corn Rootworm-Protected Corn Event MON 863 Is Equivalent to That of Conventional Corn (Zea mays L.)

Abstract: Insect-protected corn hybrids containing event MON 863 protect corn plants against feeding damage from corn rootworm (Diabrotica), a major North American insect pest. Corn event MON 863 contains a gene that expresses an amino acid sequence variant of the wild-type Cry3Bb1 insecticidal protein from Bacillus thuringiensis. The purpose of this study was to compare the composition of corn containing event MON 863 with that of conventional nontransgenic corn. Compositional analyses were conducted to measure proxima… Show more

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Cited by 50 publications
(37 citation statements)
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“…Moisture, crude protein, crude fat and ash contents were determined using the standard methods by the Association of Official Analytical Chemists (AOAC) with triplicate determinations (AOAC International, 2005). Mineral contents of magnesium (Mg), phosphorus (P), potassium (K), calcium (Ca), iron (Fe) and zinc (Zn) were determined by an inductively coupled plasma mass spectrometry (ICP; ICPS-8100, Shimadzu Co. Ltd., Japan) with triplicate determinations based on the method reported by George et al (2004) with slight modification. Maize flour in a porcelain crucible was preliminary ashed using a Bunsen burner until smoking had ceased, and then it was dry-ashed using a muffle furnace (FM-35, Yamato Scientific Co., Ltd., Japan) at 550°C for 8 h. The resultant ash was dissolved in 100 mL of 2N-HCl, and then diluted 10-fold using deionized water.…”
Section: Analytical Methods Of Chemical Propertiesmentioning
confidence: 99%
“…Moisture, crude protein, crude fat and ash contents were determined using the standard methods by the Association of Official Analytical Chemists (AOAC) with triplicate determinations (AOAC International, 2005). Mineral contents of magnesium (Mg), phosphorus (P), potassium (K), calcium (Ca), iron (Fe) and zinc (Zn) were determined by an inductively coupled plasma mass spectrometry (ICP; ICPS-8100, Shimadzu Co. Ltd., Japan) with triplicate determinations based on the method reported by George et al (2004) with slight modification. Maize flour in a porcelain crucible was preliminary ashed using a Bunsen burner until smoking had ceased, and then it was dry-ashed using a muffle furnace (FM-35, Yamato Scientific Co., Ltd., Japan) at 550°C for 8 h. The resultant ash was dissolved in 100 mL of 2N-HCl, and then diluted 10-fold using deionized water.…”
Section: Analytical Methods Of Chemical Propertiesmentioning
confidence: 99%
“…Previous studies for two insect-protected products in maize, MON 810 11 and MON 863, 12 and a herbicide-tolerant maize product, NK603, 13 . MON 810 contains a gene that produces a variant of the insecticidal protein Cry1Ab derived from Bacillus thuringiensis and confers resistance to the European corn borer (ECB) and other lepidopteran insect pests.…”
Section: ' Introductionmentioning
confidence: 99%
“…El contenido de los metales determinados tanto en grano total como en pericarpio se encontro´dentro de los intervalos citados en la literatura para maı´z amarillo en grano total (George et al, 2004), con excepcio´n del Cu que en el pericarpio que se encuentra 3 veces sobre ma´s concentrado debido probablemente a la diferencia de variedad.…”
Section: Resultados Y Discusio´nunclassified
“…En la Tabla 3 se presentan las longitudes de onda de emisio´n que se trabajaron para cada uno de los elementos analizados, de acuerdo con el valor recomendado por el fabricante del equipo (Perkin-Elmer). Debido a que en el maı´z criollo azul la coloracio´n se presenta principalmente en el pericarpio, se analizaron los metales ma´s comu´nmente encontrados en maı´z (George et al, 2004), determina´ndose el contenido en grano total (incluyendo pericarpio) y pericarpio. No se realizo´el ana´lisis del grano sin el pericarpio debido a que el contenido de antocianinas en e´ste no es significativo, comparado con el pericarpio, por lo que el efecto de los metales en la estabilidad de las antocianinas no se apreciarı´a.…”
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