Compositional distribution of hydroxyapatite surface and interface observed by electron spectroscopy

Suetsugu, Yasushi; Hirota, K.; Fujii, Kazuko; Tanaka, Junzo

doi:10.1007/bf00366349

Cited by 19 publications

(9 citation statements)

References 5 publications

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“…The carbon 1s peak is attributed to the carbonate apatitic environment of bone and not to beam-induced carbon contamination, which is generally not observed in ground homogenized materials [38]. Bone sections, however, usually sustain this effect, probably due to the evolution of a Ca-deficient carbonated apatitic phase at the surface and the grain boundaries of bone hydroxyapatite polycrystalline specimens [62]. Such an impurity adsorption EDX spectrum is also typical of hydroxyapatite-like samples featuring distinct (relative to the X-ray continuum) characteristic peaks at 3.7 KeV for Ca and 2.0 Kev for P, and consistent Kα emission lines (Fig.…”

Section: Resultsmentioning

confidence: 99%

Ca/P concentration ratio at different sites of normal and osteoporotic rabbit bones evaluated by Auger and energy dispersive X-ray spectroscopy

2011

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Osteoporosis is a systemic skeletal disorder associated with reduced bone mineral density and the consequent high risk of bone fractures. Current practice relates osteoporosis largely with absolute mass loss. The assessment of variations in chemical composition in terms of the main elements comprising the bone mineral and its effect on the bone's quality is usually neglected. In this study, we evaluate the ratio of the main elements of bone mineral, calcium (Ca), and phosphorus (P), as a suitable in vitro biomarker for induced osteoporosis. The Ca/P concentration ratio was measured at different sites of normal and osteoporotic rabbit bones using two spectroscopic techniques: Auger electron spectroscopy (AES) and energy-dispersive X-ray spectroscopy (EDX). Results showed that there is no significant difference between samples from different genders or among cortical bone sites. On the contrary, we found that the Ca/P ratio of trabecular bone sections is comparable to cortical sections with induced osteoporosis. Ca/P ratio values are positively related to induced bone loss; furthermore, a different degree of correlation between Ca and P in cortical and trabecular bone is evident. This study also discusses the applicability of AES and EDX to the semiquantitative measurements of bone mineral's main elements along with the critical experimental parameters.

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Section: Resultsmentioning

confidence: 99%

Ca/P concentration ratio at different sites of normal and osteoporotic rabbit bones evaluated by Auger and energy dispersive X-ray spectroscopy

2011

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“…6). It is well known that even if a carbonate-free solution is used, 34,35 carbonate is incorporated into apatite from the atmosphere. This phenomenon, a decrease of HPO 4 2− ions while carbonate ions remains, is also shown when apatite prepared in an aqueous solution is treated with heat.…”

Section: Discussionmentioning

confidence: 99%

Preparation and characterization of apatite deposited on silk fabric using an alternate soaking process

Furuzono¹,

Taguchi²,

Kishida³

et al. 2000

J. Biomed. Mater. Res.

106

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Apatite-deposited silk fabric composite materials were developed using a new alternate soaking process. The characteristics of deposited apatite were studied using scanning electron microscopy (SEM), X-ray diffraction (XRD), Fourier transform infrared spectrophotometry (FTIR), and X-ray photoelectron spectroscopy (XPS). Apatite weight increased with alternating soaking in a calcium solution [200 mM aqueous calcium chloride solution buffered with tris(hydroxymethyl) aminomethane and HCl (pH 7.4)] and a phosphate solution (120 mM aqueous disodium hydrogenphosphate) changed every hour. SEM showed that apatite deposited after 21 or more repeated soakings was over 20 microm thick. XRD showed that with alternate soakings, the apatite crystals deposited on silk fabric elongated along the c axis. FTIR and XPS indicated the existence of carbonate, HPO(4)(2-), and Na(+) ions in addition to constituent ions of hydroxyapatite. A loss of HPO(4)(2-) and Na(+) ions in the deposit upon further soaking might be associated with an increasing apatite crystallinity. Apatite deposited on silk by the alternate soaking process was a deficient apatite containing carbonate, HPO(4)(2-), and Na(+) ions as in a natural bone tissue. Thus, this apatite-silk composite material might be potentially bioactive.

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“…The bands at 1422 and 872 cm À1 are derived from carbonate ions, which indicate that PO 4 3À sites in FB50-HA are replaced partially by carbonate ions. It is well known that even if a carbonate-free solution is used [18,19], carbonate can be incorporated into apatite from the atmosphere.…”

Section: Ftir Spectramentioning

confidence: 99%

Silk fibroin regulated mineralization of hydroxyapatite nanocrystals

Kong

Cui

Wang

et al. 2004

Journal of Crystal Growth

161

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Compositional distribution of hydroxyapatite surface and interface observed by electron spectroscopy

Cited by 19 publications

References 5 publications

Ca/P concentration ratio at different sites of normal and osteoporotic rabbit bones evaluated by Auger and energy dispersive X-ray spectroscopy

Ca/P concentration ratio at different sites of normal and osteoporotic rabbit bones evaluated by Auger and energy dispersive X-ray spectroscopy

Preparation and characterization of apatite deposited on silk fabric using an alternate soaking process

Silk fibroin regulated mineralization of hydroxyapatite nanocrystals

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