Compound 48/80 and the healing of wounds in rats. The effect of timing of drug injections

Abstract

Wound breaking strength was measured in Long-Evans rats in two settings: in one experiment animals received Compound 48/80 i.p. for 8 consecutive days, starting 1 day before infliction of wound and in the second experiment 48/80 was given for 6 days before wounding. Wound breaking strength was always measured on the seventh day after wounding. There was a significant increase in wound breaking strength in animals treated before incision was made, as compared to controls, saline-treated animals (p less than 0.01). When 48/80 was given one day before incision was made, the breaking strength was significantly decreased (p less than 0.0002). Possible explanation for these opposite results is discussed.

Histamine H4 receptors on mammary epithelial cells of the human breast with different types of carcinoma

“…Several studies have also suggested an immunologically protective role for mast cells against tumours [6]. However, increasing evidence has supported a more direct interaction of mast cells with tumour cells, especially interactions that facilitate rapid tumour growth [7].…”

Experimental mast cell activation improves connective tissue repair in the perforated rat mesentery

“…However, an increased formation of histamine (increase of histamine forming capacity) has been described after both wounding and activation of mast cells by Compound 48/80 [24,25]. Preoperative, compared to postoperative, mast cell activation with Compound 48/80 has been shown to enhance healing [6] and in agreement with those findings, we recorded significantly better healing in rats who had received Compound 48/80 for five days prior to operation than in rats who received such treatment five days postoperatively. It, therefore, is conceivable that histamine might have a significant influence on healing.…”

“…Another advantage is that healing takes place inside the body in an environment with a constant high humidity. Relationships between mast cells and different characteristics of healing, such as tensile strength [5,6,9] and collagen deposition [5], have been described. In contrast to those investigations, we quantitated healing as such by estimating the percentage of closed perforations among twenty wounds per animal.…”

“…An activation also stimulates different events such as angiogenesis and flbroblast and mesothelial cell proliferation in the rat mesentery [1][2][3], as well as angiogenesis in the chick chorioallantoic membrane [4]. Cell proliferation and angiogenesis are of vital importance for an unperturbed tissue repair, and in fact, mast cells have repeatedly been associated with wound healing [5][6][7][8][9] and conditions with chronic inflammation and fibrosis [10,11]. In the present investigation we used the perforatedrat-mesentery model [12] to study mast cell participation in connective tissue repair.…”

Effect of cimetidine on wound healing in rats

“…There is evidence to suggest that endogenous nascent histamine stimulates wound healing as shown by several investigators [1][2][3][4][5][6]. Recently it has been shown by Dabrowski and Maslinski [7] that exogenous histamine and 4-methistamine (an H-2 agonist) injected into wounds have a stimulatory effect in low doses and an inhibitory effect in high doses on reparative collagen accumulation.…”