Compound Radix Sophorae Flavescentis exerts antitumor effects by inhibiting the proliferation and inducing the apoptosis of esophageal carcinoma TE-8 cells

Yang, Xiaoyu; Cai, Weimei; Yang, Qianqian; Lu, Zhihong; Li, Jinsong; Yu, Ji-Guo

doi:10.3892/ol.2015.3607

Cited by 11 publications

(10 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Combined chemotherapy can effectively reduce or stabilize the tumours, improve quality of life, and significantly alleviate pain due to cancer [16]. Compound Sophora injection has occasional adverse reactions to rashes in the clinic [16,17]. However, it is not clear whether compound Sophora injection can reverse the resistance.…”

Section: Introductionmentioning

confidence: 99%

The Effect of Compound Sophora on Fluorouracil and Oxaliplatin Resistance in Colorectal Cancer Cells

Zhong

Fan

et al. 2019

Evidence-Based Complementary and Alternative Medicine

View full text Add to dashboard Cite

Fluorouracil (5-FU) and oxaliplatin (L-OHP) are the most commonly used chemotherapy drugs for colorectal cancer, though resistance is common. Compound Sophora injection is a traditional Chinese medicine that can protect the liver against oxidation, improve immunity, and enhance sensitivity to chemotherapy; it may have an effect of reversing resistance in 5-FU- and L-OHP-resistant gastric cancer cells (5-FU/SW480 and L-OHP/SW480, respectively). A concentration gradient experiment was performed to identify a nontoxic dose of compound Sophora injection. 5-FU/SW480 and L-OHP/SW480 cells were treated with the nontoxic dose of compound radix Sophorae injection for 48 h, and changes in drug resistance to 5-FU and L-OHP were detected. Alterations in apoptosis and the cell cycle were assessed, as were the mRNA and protein levels of permeability glycoprotein (P-gp), annexin A1 (ANXA1), and ATP-binding cassette superfamily G member 2 (ABCG2). Flow cytometry showed a reduction in the number of cells in the G1 phase and an increase of cells in the S phase (P<0.05). mRNA and protein expression of P-gp and ABCG2 was significantly higher in 5-FU/SW480 and L-OHP/SW480 cell lines, and ANXA1 expression decreased significantly (P<0.05). Compound Sophora injection can reverse the drug resistance of 5-FU/SW480 and L-OHP/SW480 cell lines to 5-FU and L-OHP, respectively, possibly through a mechanism involving reduced expression of P-gp and ABCG2 but enhanced expression of ANXA1, which is the basis for the identification of clinical drug resistance in colorectal cancer.

show abstract

Section: Introductionmentioning

confidence: 99%

The Effect of Compound Sophora on Fluorouracil and Oxaliplatin Resistance in Colorectal Cancer Cells

Zhong

Fan

et al. 2019

Evidence-Based Complementary and Alternative Medicine

View full text Add to dashboard Cite

show abstract

“…Radix Sophorae Flavescentis (RSF) is an ancient Chinese herb (8) that contains active ingredients such as sophordine, matrine, and sophocarpine (8,9). RSF has detoxification, diuretic, and insect repellent properties (10). It is also one of the important ingredients for the treatment of contact dermatitis and local pruritus of the vagina (11,12).…”

Section: Introductionmentioning

confidence: 99%

“…It also has other pharmacological properties, such as antibacterial, anti-tumour, and anti-viral (against hepatitis B virus) effects (12)(13)(14). RSF induces apoptosis of laryngeal neoplasm Hep2 cells (15), esophageal carcinoma TE-8 cells (10), and PC-3 prostate cancer cells (16). However, evidence is minimal in order to recommend RSF for use in the treatment of gastric cancers.…”

Section: Introductionmentioning

confidence: 99%

Radix Sophorae Flavescentis inhibits proliferation and induces apoptosis of AGS human gastric cancer cells

et al. 2018

View full text Add to dashboard Cite

Traditional herbal medicines are being increasingly used worldwide to treat cancer. Radix Sophorae Flavescentis (RSF) is a Chinese herb, which has numerous pharmacological properties, including anti-tumour effects. In this study, we investigated the mechanisms underlying RSF-induced apoptosis in human gastric cancer cells (AGS cells). We found that RSF treatment (20-200 µg/ml) inhibited the proliferation of AGS cells and increased the sub-G1 phase ratio. RSF-induced cell death was associated with the downregulation of BCl-2 and upregulation of Bax. In addition to increasing the expression levels of apoptosis-mediating surface antigen FAS and Fas ligand, RSF also activated caspase-3; however, mitogen-activated protein kinase appeared to inhibit RSF-induced cell death. RSF also led to an increased production of reactive oxygen species. Based on these results, we propose that RSF could be a potential therapeutic agent for gastric cancer.

show abstract

“…The root of S. flavescens has been historically employed in traditional Chinese medicine, and its active constituents include alkaloids and flavonoids (MA et al, 2008;LIU et al, 2010;CHEN, 2013;ZHENG et al, 2014). These active compounds have a variety of pharmacological activities, which include anti-tumor, anti-bacterial, anti-inflammatory, and analgesic effects (JIN et al, 2010;SUN et al, 2012;WANG et al, 2015;YANG et al, 2015). Despite these extensive studies in humans and other animals, investigations on the RPS13s in plants are limited.…”

Section: Introductionmentioning

confidence: 99%

Cloning, expression, and characterization of three ribosomal protein S13 genes in Sophora flavescens

Liao¹,

Liu²,

Li³

et al. 2018

Biosci. J.

View full text Add to dashboard Cite

To characterize the structure and function of ribosomal protein S13 (RPS13), we identified fulllength open reading frames (ORFs) of three RPS13 genes (RPS13-1, RPS13-2, and RPS13-3) of the Chinese medicinal plant, Sophora flavescens. The target genes were amplified by reverse transcription-polymerase chain reaction (RT-PCR), ligated into the pET22b(+) vector, and then transformed into Escherichia coli BL21 competent cells for protein expression. The physicochemical properties, protein motif, evolution, and structural organization of the three RPS13 genes were analyzed using bioinformatics tools. The full-length ORFs (453 bp) of the three RPS13 genes of S. flavescens were cloned, and each encodes a protein of 151 amino acids in length, and their expression was detected by Western blotting. Bioinformatics analysis showed that RPS13s are stable proteins that are closely related to the 40S RPS13s of Vigna radiate var. radiate. Their three-dimensional structures included three α-helices at the C-terminal and four α-helices at the N-terminal, and the two clusters of helices were connected by a long random coil, which may help maintain the dynamic bridging interactions between the large and small subunits of the ribosome. The full-length ORFs of three RPS13 genes of S. flavescens were successfully cloned and expressed in vitro. The study of the physicochemical properties, evolution, and secondary and three-dimensional structures of the three proteins will provide the theoretical basis for further studies on the function of RPS13s in plants.

show abstract

Compound Radix Sophorae Flavescentis exerts antitumor effects by inhibiting the proliferation and inducing the apoptosis of esophageal carcinoma TE-8 cells

Cited by 11 publications

References 10 publications

The Effect of Compound Sophora on Fluorouracil and Oxaliplatin Resistance in Colorectal Cancer Cells

The Effect of Compound Sophora on Fluorouracil and Oxaliplatin Resistance in Colorectal Cancer Cells

Radix Sophorae Flavescentis inhibits proliferation and induces apoptosis of AGS human gastric cancer cells

Cloning, expression, and characterization of three ribosomal protein S13 genes in Sophora flavescens

Contact Info

Product

Resources

About