Comprehensive analysis and expression profiles of cassava UDP-glycosyltransferases (UGT) family reveal their involvement in development and stress responses in cassava

Wu, Chunlai; Dai, Jing; Chen, Zhisheng; Tie, Weiwei; Yan, Yan; Yang, Hai; Zeng, Jian; Hu, Wei

doi:10.1016/j.ygeno.2021.08.004

Cited by 15 publications

(9 citation statements)

References 75 publications

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“…Moreover, we obtained a total of 2,203 duplicated gene pairs in four cotton species, including 276 segmental duplications and 146 tandem duplications ( Figure 6 ). WGD, segmental duplication and tandem duplication have all played important roles in the large-scale amplification of the cotton UGT gene family, which is consistent with previous reports ( Wu et al, 2021 ).…”

Section: Discussionsupporting

confidence: 92%

“…UGTs are widespread in all plants. Compared with Arabidopsis ( Ross et al, 2001 ), Zea mays ( Li et al, 2014 ), Cicer arietinum ( Sharma et al, 2014 ), Linum usitatissimum ( Barvkar et al, 2012 ), Brassica rapa ( Yu et al, 2017 ), Prunus mume ( Zhang et al, 2018 ), Triticum aestivum ( He et al, 2018 ), Manihot esculenta ( Wu et al, 2021 ), G. hirsutum and G. barbadense contain the highest number of UGTs . This may be because the allopolyploid G. hirsutum and G. barbadense have undergone whole-genome duplication, as well as a large number of segmental duplications and tandem duplications during evolution ( Malik et al, 2022 ).…”

Section: Discussionmentioning

confidence: 99%

“…UGT family genes have a conserved sequence of 44 amino acids in the C-terminal region, known as the plant secondary product glycosyltransferase (PSPG) motif ( Ross et al, 2001 ; Yonekura-Sakakibara and Hanada, 2011 ). Genome-wide analysis has identified numerous UGT family members in multiple species, including 107 in Arabidopsis ( Ross et al, 2001 ), 147 in Zea mays ( Li et al, 2014 ), 96 in Cicer arietinum ( Sharma et al, 2014 ), 137 in Linum usitatissimum ( Barvkar et al, 2012 ), 130 in Prunus mume ( Zhang et al, 2018 ), 179 in Triticum aestivum ( He et al, 2018 ), and 121 in Manihot esculenta ( Wu et al, 2021 ). The Arabidopsis UGT gene family is divided into 14 distinct clades (A-N) ( Li et al, 2001 ; Ross et al, 2001 ).…”

Section: Introductionmentioning

confidence: 99%

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Genome-wide identification, evolution and function analysis of UGTs superfamily in cotton

Sun

Zhao

Huang

et al. 2022

Front. Mol. Biosci.

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Glycosyltransferases mainly catalyse the glycosylation reaction in living organisms and widely exists in plants. UGTs have been identified from G. raimondii, G. arboreum and G. hirsutum. However, Genome-wide systematic analysis of UGTs superfamily have not been studied in G. barbadense. 752 UGTs were identified from four cotton species and grouped into 18 clades, of which R was newly discovered clades. Most UGTs were clustered at both ends of the chromosome and showed a heterogeneous distribution. UGT proteins were widely distributed in cells, with the highest distribution in chloroplasts. UGTs of the same clade shared similar intron/exon structural features. During evolution, the gene family has undergone strong selection for purification. UGTs were significantly enriched in “transcriptional activity (GO:0016758)” and “metabolic processes (GO:0008152)”. Genes from the same clade differed in function under various abiotic stresses. The analysis of cis-acting element and qRT–PCR may indicate that GHUGTs play important roles in plant growth, development and abiotic stress. We further found that GHUGT74-2 plays an important role under submergence. The study broadens the understanding of UGTs in terms of gene characteristics, evolutionary processes, and gene function in cotton and provides a new way to systematically and globally understand the structure–function relationship of multigene families in the evolutionary process.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Genome-wide identification, evolution and function analysis of UGTs superfamily in cotton

Sun

Zhao

Huang

et al. 2022

Front. Mol. Biosci.

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“…Glyma.13G056600 , a gene corresponding to Cluster 3 in the leaves, encodes a UDP-Glycosyltransferase (UGT) superfamily protein. UGT family genes are widely involved in plant growth and stress responses, and are found in a variety of plants [ 42 , 43 , 44 ]. In fact, the overexpression of various UGT genes in Arabidopsis has been shown to increase several stress tolerance, but some have also been reported to increase sensitivity [ 42 ].…”

Section: Discussionmentioning

confidence: 99%

Transcriptome Profiling of a Soybean Mutant with Salt Tolerance Induced by Gamma-ray Irradiation

Kang,

Chowdhury,

Kang

et al. 2024

Plants

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Salt stress is a significant abiotic stress that reduces crop yield and quality globally. In this study, we utilized RNA sequencing (RNA-Seq) to identify differentially expressed genes (DEGs) in response to salt stress induced by gamma-ray irradiation in a salt-tolerant soybean mutant. The total RNA library samples were obtained from the salt-sensitive soybean cultivar Kwangan and the salt-tolerant mutant KA-1285. Samples were taken at three time points (0, 24, and 72 h) from two tissues (leaves and roots) under 200 mM NaCl. A total of 967,719,358 clean reads were generated using the Illumina NovaSeq 6000 platform, and 94.48% of these reads were mapped to 56,044 gene models of the soybean reference genome (Glycine_max_Wm82.a2.v1). The DEGs with expression values were compared at each time point within each tissue between the two soybeans. As a result, 296 DEGs were identified in the leaves, while 170 DEGs were identified in the roots. In the case of the leaves, eight DEGs were related to the phenylpropanoid biosynthesis pathway; however, in the roots, Glyma.03G171700 within GmSalt3, a major QTL associated with salt tolerance in soybean plants, was differentially expressed. Overall, these differences may explain the mechanisms through which mutants exhibit enhanced tolerance to salt stress, and they may provide a basic understanding of salt tolerance in soybean plants.

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“…The tandem and segmental duplications of the genome provided more gene copies for generating novel gene functions and expanding gene families in alfalfa. However, compared with the seven segmental duplications in sacred lotus ( Nelumbo nucifera ) ( Li H. et al, 2020 ), 24 gene segmental duplication events in cassava ( Manihot esculenta Crantz) ( Wu et al, 2021 ), and 28 segmental duplications in Broussonetia papyrifera ( Wang et al, 2021 ), alfalfa has fewer segmental duplication genes and only one pair of highly homologous genes involving MsUGT4 and MsUGT182 . This indicates that tandem duplication events are more crucial than segmental duplications for MsUGT expansion.…”

Section: Discussionmentioning

confidence: 99%

Genome-wide identification, characterization, and expression analysis of UDP-glycosyltransferase genes associated with secondary metabolism in alfalfa (Medicago sativa L.)

Jiang²,

Sun³

et al. 2022

Front. Plant Sci.

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Uridine diphosphate glycosyltransferases (UGTs) are enzymes that catalyze glycosylation modifications and play an essential role in regulating plant metabolism. Alfalfa (Medicago sativa L.) is the most important legume in the world due to its high yields and protein content; however, the UGT genes in alfalfa have not yet been studied. Identifying UGT genes with metabolic roles in alfalfa is essential for identifying and modifying genetic traits that are relevant to yield and quality. In this study, 90 of the 239 UGT genes identified from the alfalfa “Zhongmu No. 1” genome database were found to be related to secondary metabolism, and a series of gene family characterization analyses were conducted on each. The results demonstrated that all 90 UGT genes were unevenly distributed on eight chromosomes with few introns and that tandem duplications were the crucial driving force expanding the UGT family in alfalfa. Notably, the 90 UGT genes can be clustered into ten evolutionary groups which contain specific PSPG motifs, and genes in these ten groups have specific tissue expressions. This suggests that the UGT genes in each group could have similar glycosylation roles corresponding to analogous secondary metabolites in alfalfa. Additionally, multiple cis-acting elements found in MsUGT promoter regions, such as phytohormone and flavonoids, indicate that 90 UGT members could be induced by these features, which are also related to secondary metabolism. Therefore, our study identified 90 UGT members inten evolutionary groups that are likely related to glycosylation modifications with secondary metabolites in alfalfa. These findings help uncover pivotal regulatory mechanisms associated with secondary metabolism in plant yield and quality and contribute to genetic modification and breeding in alfalfa and other plant species.

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Comprehensive analysis and expression profiles of cassava UDP-glycosyltransferases (UGT) family reveal their involvement in development and stress responses in cassava

Cited by 15 publications

References 75 publications

Genome-wide identification, evolution and function analysis of UGTs superfamily in cotton

Genome-wide identification, evolution and function analysis of UGTs superfamily in cotton

Transcriptome Profiling of a Soybean Mutant with Salt Tolerance Induced by Gamma-ray Irradiation

Genome-wide identification, characterization, and expression analysis of UDP-glycosyltransferase genes associated with secondary metabolism in alfalfa (Medicago sativa L.)

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