Comprehensive analysis of antioxidant mechanisms in Arabidopsis glutathione peroxidase-like mutants under salt- and osmotic stress reveals organ-specific significance of the AtGPXL’s activities

Bela, Krisztina; Riyazuddin, Riyazuddin; Horváth, Edit; Hurton, Ágnes; Gallé, Ágnes; Takács, Zoltan; Zsigmond, Laura; Szabados, László; Tari, Irma; Csiszár, Jolán

doi:10.1016/j.envexpbot.2018.02.016

Cited by 35 publications

(45 citation statements)

References 94 publications

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“…In addition to the increase in the enzymatic antioxidants in 'Umsila', there was also an increase in non-enzymatic antioxidants such as GS, TPC, TFC, and proline, due to salinity. In the roots of 'Umsila', there was a significant increase in the GS (Figure 6A), probably as a result of the activation of a redox homeostasis mechanism such as the ASC-GSH cycle, in response to salt stress [74][75][76]. The root-specific increase of GS in 'Umsila' could be due to an increase in the demand for sulfur ions under salinity stress.…”

Section: Discussionmentioning

confidence: 98%

Antioxidant Response to Salinity in Salt-Tolerant and Salt-Susceptible Cultivars of Date Palm

2019

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The salinity tolerance mechanism in date palm through antioxidation has not been completely deciphered to date. Therefore, this study aimed to investigate the role of various antioxidants in salinity tolerance. Two date palm cultivars, contrasting in salinity tolerance, were used as model plants in a comparative study designed to detect changes in growth, Na+ and K+ uptake, reactive oxygen species (ROS), and antioxidant accumulations, when plants were exposed to salt stress. The results showed that salinity treatment had a more substantial negative effect on the growth and photosynthetic pigmentation of the susceptible ‘Zabad’ cultivar than on the tolerant ‘Umsila’ cultivar, probably due to the ability of ‘Umsila’ to accumulate less Na+ and more K+, to maintain a normal concentration of ROS and to produce more non-enzymatic antioxidants, including glutathione, phenolic compounds, flavonoids, and proline. Under salinity, ‘Umsila’ could also activate more superoxide dismutase (SOD), catalase (CAT) and ascorbate peroxidase (APX) than ‘Zabad’. These results suggest that the tolerance of ‘Umsila’ is partially due to the balanced Na+ and K+ uptake and to the relatively high concentration of ROS-scavenging metabolites. Together, these results indicate that the antioxidant mechanism is crucial for salinity tolerance in date palms. However, other mechanisms may also be involved in this trait.

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Section: Discussionmentioning

confidence: 98%

Antioxidant Response to Salinity in Salt-Tolerant and Salt-Susceptible Cultivars of Date Palm

2019

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“…In the light of these findings it is rather surprising that A. thaliana GPXL3 has been described as a component of cytosolic abscisic acid (ABA) signaling and drought stress responses (Miao et al ., ). Independent experiments have failed to reproduce the drought‐sensitive phenotype reported for gpxl3 null mutants (Bela et al ., ). The gpxl3 mutants have been reported to have a lower lateral root density than wild‐type (WT) plants but showed similar responses to treatments with auxin, strigolactone and ABA in terms of root architecture (Passaia et al ., ).…”

Section: Formation and Isomerization Of Disulfides In The Er And The mentioning

confidence: 97%

Oxidative protein folding: state‐of‐the‐art and current avenues of research in plants

2018

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Contents Summary I. Introduction II. Formation and isomerization of disulfides in the ER and the Golgi apparatus III. The disulfide relay in the mitochondrial intermembrane space: why are plants different? IV. Disulfide bond formation on luminal proteins in thylakoids V. Conclusion Acknowledgements References SUMMARY: Disulfide bonds are post-translational modifications crucial for the structure and function of thousands of proteins. Their formation and isomerization, referred to as oxidative folding, require specific protein machineries found in oxidizing subcellular compartments, namely the endoplasmic reticulum and the associated endomembrane system, the intermembrane space of mitochondria and the thylakoid lumen of chloroplasts. At least one protein component is required for transferring electrons from substrate proteins to an acceptor that is usually molecular oxygen. For oxidation reactions, incoming reduced substrates are oxidized by thiol-oxidoreductase proteins (or domains in case of chimeric proteins), which are usually themselves oxidized by a single thiol oxidase, the enzyme generating disulfide bonds de novo. By contrast, the description of the molecular actors and pathways involved in proofreading and isomerization of misfolded proteins, which require a tightly controlled redox balance, lags behind. Herein we provide a general overview of the knowledge acquired on the systems responsible for oxidative protein folding in photosynthetic organisms, highlighting their particularities compared to other eukaryotes. Current research challenges are discussed including the importance and specificity of these oxidation systems in the context of the existence of reducing systems in the same compartments.

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“…The GR activity was determined by measuring the absorbance increment at 412 nm when 5,5'-dithio-bis(2-nitrobenzoic acid) (DTNB) was reduced by GSH, generated from GSSG [40]. The activity was calculated as the amount of reduced DTNB, in nmol min -1 mg −1 protein, ε 420 = 13.6 mM -1 cm -1 .…”

Section: Determination Of Glutathione Reductase and Dehydrosacorbate mentioning

confidence: 99%

“…Non-enzymatic scavengers of ROS include AsA, GSH, phenolic compounds, carotenoids, flavonoids and tocopherol. Enzymatic ROS scavengers include superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX), glutathione peroxidase-like (GPXL), DHAR and GST enzymes [40].…”

Section: Introductionmentioning

confidence: 99%

Compensation of Mutation in Arabidopsis glutathione transferase (AtGSTU) Genes under Control or Salt Stress Conditions

Horváth

Bela

Gallé

et al. 2020

IJMS

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Glutathione transferases (GSTs) play a crucial role in detoxification processes due to the fact of their glutathione (GSH) conjugating activity, and through glutathione peroxidase or dehydroascorbate reductase (DHAR) activities, they influence the redox state of GSH and ascorbate (AsA). The plant-specific tau (GSTU) group is the largest class of Arabidopsis GSTs, and their members are involved in responses to different abiotic stresses. We investigated the effect of salt stress on two-week-old Arabidopsis thaliana wild-type (Col-0), Atgstu19 and Atgstu24 mutant plants after applying 150 mM NaCl for two days. The Atgstu19 seedlings had lower GST activity and vitality both under control conditions and after salt stress than the wild-type, but the level of total ROS was similar to the Col-0 plants. The GST activity of the knockout Atgstu24 mutant was even higher under control conditions compared to the Col-0 plants, while the ROS level and its vitality did not differ significantly from the wild-type. Analysis of the AtGSTU expression pattern revealed that the mutation in a single AtGSTU gene was accompanied by the up- and downregulation of several other AtGSTUs. Moreover, elevated AsA and GSH levels, an altered GSH redox potential and increased DHAR and glutathione reductase activities could help to compensate for the mutation of AtGSTU genes. The observed changes in the mutants suggest that the investigated isoenzymes influence the redox homeostasis under control conditions and after NaCl treatment in Arabidopsis seedlings. These data indicate for the first time the more general role of a temporary shift of redox status as part of GST mechanisms and regulation.

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Comprehensive analysis of antioxidant mechanisms in Arabidopsis glutathione peroxidase-like mutants under salt- and osmotic stress reveals organ-specific significance of the AtGPXL’s activities

Cited by 35 publications

References 94 publications

Antioxidant Response to Salinity in Salt-Tolerant and Salt-Susceptible Cultivars of Date Palm

Antioxidant Response to Salinity in Salt-Tolerant and Salt-Susceptible Cultivars of Date Palm

Oxidative protein folding: state‐of‐the‐art and current avenues of research in plants

Compensation of Mutation in Arabidopsis glutathione transferase (AtGSTU) Genes under Control or Salt Stress Conditions

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