Comprehensive Analysis of N6-Methyladenosine (m6A) Methylation in Neuromyelitis Optica Spectrum Disorders

Hong, Yun–Chul; Wu, Yifan; Ding, Jie; Liu, Wei; Zhu, Dezhi; Shen, Xiafeng; Guan, Yangtai

doi:10.3389/fgene.2021.735454

Cited by 3 publications

(2 citation statements)

References 36 publications

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“…The conserved characteristic peak of the zebrafish m6A methylation group is preferentially distributed near the termination codon with the RRACH motif [ 32 ]. Motif analysis of the peaks within the highest scoring mRNAs revealed the presence of the consistent GGAC sequence in the three replicates from the neuromyelitis optica spectrum disorders and healthy groups [ 33 ]. In round spermatocytes (RO), 10271 m6A peaks associated with 10,109 genes were identified from wild-type mice, and 9,559 m6A peaks associated with 10,138 genes were identified from m6A eraser Alkbh5 knockout mice, with peaks concentrated in the coding region of the GGAC motif and the stop codon [ 34 ].…”

Section: Discussionmentioning

confidence: 99%

N6-methyladenosine methylation analysis of long noncoding RNAs and mRNAs in 5-FU-resistant colon cancer cells

Lai,

Zhou,

et al. 2023

Epigenetics

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N6 methyladenosine (m6A), methylation at the sixth N atom of adenosine, is the most common and abundant modification in mammalian mRNAs and non-coding RNAs. Increasing evidence shows that the alteration of m6A modification level could regulate tumour proliferation, metastasis, self-renewal, and immune infiltration by regulating the related expression of tumour genes. However, the role of m6A modification in colorectal cancer (CRC) drug resistance is unclear. Here, MeRIP-seq and RNA-seq techniques were utilized to obtain mRNA, lncRNA expression, and their methylation profiles in 5-Fluorouracil (5-FU)-resistant colon cancer HCT-15 cells and control cells. In addition, we performed detailed bioinformatics analysis as well as in vitro experiments of lncRNA to explore the function of lncRNA with differential m6A in CRC progression and drug resistance. In this study, we obtained the m6A methylomic landscape of CRC cells and resistance group cells by MeRIP-seq and RNA-seq. We identified 3698 differential m6A peaks, of which 2224 were hypermethylated, and 1474 were hypomethylated. Among the lncRNAs, 60 were hypermethylated, and 38 were hypomethylated. GO and KEGG analysis annotations showed significant enrichment of endocytosis and MAPK signalling pathways. Moreover, knockdown of lncRNA ADIRF-AS1 and AL139035.1 promoted CRC proliferation and invasive metastasis in vitro. lncRNA- mRNA network showed that ADIRF-AS1 and AL139035.1 May play a key role in regulating drug resistance formation. We provide the first m6A methylation profile in 5-FU resistance CRC cells and analyse the functions of differential m6A-modified mRNAs and lncRNAs. Our results indicated that differential m6A RNAs were significantly associated with MAPK signalling and endocytosis after induction of 5-FU resistance. Knockdown of LncRNA ADIRF-AS1 and AL139035.1 promotes CRC progression and might be critical in regulating drug resistance formation.

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Section: Discussionmentioning

confidence: 99%

N6-methyladenosine methylation analysis of long noncoding RNAs and mRNAs in 5-FU-resistant colon cancer cells

Lai,

Zhou,

et al. 2023

Epigenetics

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“…This kind of modi cation could in uence the regulation of gene expression by means of transcript decay, splicing, translation, and stability maintenance [1][2][3][4]. In addition, dysregulation of m 6 A is associated with multiple diseases, such as neuromyelitis optica spectrum disorder and oesophageal squamous cell carcinoma [5,6]. Researchers have developed an antibody-based approach, m 6 A-seq (or MeRIP-seq), to identify speci c m 6 A peaks [7].…”

Section: Introductionmentioning

confidence: 99%

N6-Methyladenosine (m6A) Methylation Is Associated with the Immune Microenvironments in Acute Intracerebral Hemorrhage (ICH)

Yang,

Xie,

et al. 2023

Mol Neurobiol

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Recently, researchers have found that N 6 -methyladenosine (m 6 A) is a kind of internal posttranscriptional modi cation that is very pivotal in mammalian mRNA. However, the features of m 6 A RNA methylation in acute intracerebral haemorrhage (ICH) are still not known. To explore differential methylation modi cations and to discover their functions in acute ICH patients. We recruited three acute ICH patients, three healthy controls and an additional three patients and healthy controls for validation. m 6 A methylation levels were determined by ultrahigh-performance liquid chromatography coupled with triple quadruple mass spectrometry (UPLC-QQQ-MS) in blood samples from the two groups. Methylated RNA immunoprecipitation sequencing (MeRIP-seq) was employed to identify differences in m 6 A modi cation. Differentially expressed m 6 A-modi ed genes were con rmed by MeRIP-qPCR. We found that there were no signi cant differences in total m 6 A levels between the two groups. However, we observed differential methylation peaks. Compared with the control group, the coding genes showing increased methylation following acute ICH were mostly harboured in processes connected with osteoclast differentiation, the neurotrophin signalling pathway and the spliceosome, while genes with reduced m 6 A modi cation were harboured in the B-cell receptor signalling pathway and the T-cell receptor signalling pathway. These results reveal that differentially m 6 A-modi ed genes may in uence immune microenvironments in acute ICH.

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N 6 -methyladenosine (m6A) methylation is associated with immune microenvironments in acute intracerebral haemorrhage(ICH)

Yang

Xie

et al. 2023

Preprint

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Recently, researchers have found that N6-methyladenosine (m6A) is a kind of internal posttranscriptional modification that is very pivotal in mammalian mRNA. However, the features of m6A RNA methylation in acute intracerebral haemorrhage (ICH) are still not known. To explore differential methylation modifications and to discover their functions in acute ICH patients. We recruited three acute ICH patients, three healthy controls and an additional three patients and healthy controls for validation. m6A methylation levels were determined by ultrahigh-performance liquid chromatography coupled with triple quadruple mass spectrometry (UPLC-QQQ-MS) in blood samples from the two groups. Methylated RNA immunoprecipitation sequencing (MeRIP-seq) was employed to identify differences in m6A modification. Differentially expressed m6A-modified genes were confirmed by MeRIP-qPCR. We found that there were no significant differences in total m6A levels between the two groups. However, we observed differential methylation peaks. Compared with the control group, the coding genes showing increased methylation following acute ICH were mostly harboured in processes connected with osteoclast differentiation, the neurotrophin signalling pathway and the spliceosome, while genes with reduced m6A modification were harboured in the B-cell receptor signalling pathway and the T-cell receptor signalling pathway. These results reveal that differentially m6A-modified genes may influence immune microenvironments in acute ICH.

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Comprehensive Analysis of N6-Methyladenosine (m6A) Methylation in Neuromyelitis Optica Spectrum Disorders

Cited by 3 publications

References 36 publications

N6-methyladenosine methylation analysis of long noncoding RNAs and mRNAs in 5-FU-resistant colon cancer cells

N6-methyladenosine methylation analysis of long noncoding RNAs and mRNAs in 5-FU-resistant colon cancer cells

N6-Methyladenosine (m6A) Methylation Is Associated with the Immune Microenvironments in Acute Intracerebral Hemorrhage (ICH)

N 6 -methyladenosine (m6A) methylation is associated with immune microenvironments in acute intracerebral haemorrhage(ICH)

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