Comprehensive analysis of the regulatory network of blue‐light‐regulated conidiation and hydrophobin production in <i>Trichoderma guizhouense</i>

Li, Yifan; Sun, Tingting; Guo, Degang; Gao, Jia; Zhang, Jian; Cai, Feng; Fischer, Reinhard; Yu, Zhenzhong

doi:10.1111/1462-2920.15748

Cited by 11 publications

(32 citation statements)

References 58 publications

(96 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…MAPK HOG pathway is crucial for blue light signaling in T. guizhouense [24]. By reanalyzing the transcriptome published previously [24], we found that transcript abundance of ssk2 (OPB39222), pbs2 (OPB41576) and hog1 (OPB38173) encoding components of HOG pathway were all upregulated after growth under blue light (Figure 5A).…”

Section: Blue Light Upregulates the Expression Of The Genes Encoding Hog Pathway Componentsmentioning

confidence: 62%

“…Wild type of Trichoderma guizhouense NJAU 4742 and three mutants, ∆blr1, ∆env1 and ∆hog1, which were constructed previously [24], were used in this study. The blue light receptors BLR1 and ENV1 are absent in the ∆blr1and the ∆env1-mutant strains, respectively.…”

Section: Strains and Culture Conditionsmentioning

confidence: 99%

“…Conidial yield was quantified as previously described by Li et al [24]. The wild type strain was revived on PDA medium for two days at 28 • C in the dark and afterwards fresh mycelia were inoculated on PDA medium (Ø 6 cm petri dish) using a hole puncher.…”

Section: Quantification Of Conidiamentioning

confidence: 99%

“…Transcriptome data of T. guizhouense wild type and the mutants ∆blr1, ∆env1 and ∆hog1 in response to blue light are available online in the NCBI database Sequencing Read Archive (SRA) under the accession number PRJNA743899. The genes encoding proteins putatively involved in stress resistance were screened from the differentially expressed genes (DEGs) identified in a previous study [24]. The reliability of the transcriptome data was validated by qPCR.…”

Section: Transcriptome Analysismentioning

confidence: 99%

“…Recently, we provided evidence that the blue light receptor BLR1, the white collar 1 orthologue, dominates conidiation in T. guizhouense and the blue light receptor ENV1, the orthologue of VVD, modulates photoadaptation [24]. BLR1 employs the stress-activated MAPK HOG pathway, which is also responsible for the sensing of osmotic, oxidative, and heat-shock stresses in A. nidulans, to activate a large proportion of blue light-regulated genes [24][25][26][27][28][29]. However, how other monochromatic light affects conidiation in T. guizhouense remains unknown.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

A Simple and Low-Cost Strategy to Improve Conidial Yield and Stress Resistance of Trichoderma guizhouense through Optimizing Illumination Conditions

Meng

Guo

et al. 2022

JoF

Self Cite

View full text Add to dashboard Cite

Light is perceived by photoreceptors in fungi and further integrated into the stress-activated MAPK HOG pathway, and thereby potentially activates the expression of genes for stress responses. This indicates that the precise control of light conditions can likely improve the conidial yield and stress resistance to guarantee the low cost and long shelf life of Trichoderma-based biocontrol agents and biofertilizers. In this study, effects of wavelengths and intensities of light on conidial yield and stress tolerance to osmotic, oxidative and pH stresses in Trichoderma guizhouense were investigated. We found that 2 μmol photons/(m2 × s) of blue light increased the conidial yield more than 1000 folds as compared to dark condition and simultaneously enhanced conidial stress resistance. The enhanced conidial stress resistance is probably due to the upregulated stress-related genes in blue light, which is under the control of the blue light receptor BLR1 and the MAP kinase HOG1.

show abstract

Section: Blue Light Upregulates the Expression Of The Genes Encoding Hog Pathway Componentsmentioning

confidence: 62%

Section: Strains and Culture Conditionsmentioning

confidence: 99%

Section: Quantification Of Conidiamentioning

confidence: 99%

Section: Transcriptome Analysismentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

A Simple and Low-Cost Strategy to Improve Conidial Yield and Stress Resistance of Trichoderma guizhouense through Optimizing Illumination Conditions

Meng

Guo

et al. 2022

JoF

Self Cite

View full text Add to dashboard Cite

show abstract

The bZIP transcription factor ATF1 regulates blue light and oxidative stress responses in Trichoderma guizhouense

Li,

et al. 2023

mLife

Self Cite

View full text Add to dashboard Cite

In several filamentous fungi, incident light and environmental stress signaling share the mitogen‐activated protein kinase (MAPK) HOG (SAK) pathway. It has been revealed that short‐term illumination with blue light triggers the activation of the HOG pathway in Trichoderma spp. In this study, we demonstrate the crucial role of the basic leucine zipper transcription factor ATF1 in blue light responses and signaling downstream of the MAPK HOG1 in Trichoderma guizhouense. The lack of ATF1 severely impaired photoconidiation and delayed vegetative growth and conidial germination. Upon blue light or H2O2 stimuli, HOG1 interacted with ATF1 in the nucleus. Genome‐wide transcriptome analyses revealed that 61.8% (509 out of 824) and 85.2% (702 out of 824) of blue light‐regulated genes depended on ATF1 and HOG1, respectively, of which 58.4% (481 out of 824) were regulated by both of them. Our results also show that blue light promoted conidial germination and HOG1 and ATF1 played opposite roles in controlling conidial germination in the dark. Additionally, the lack of ATF1 led to reduced oxidative stress resistance, probably because of the downregulation of catalase‐encoding genes. Overall, our results demonstrate that ATF1 is the downstream component of HOG1 and is responsible for blue light responses, conidial germination, vegetative growth, and oxidative stress resistance in T. guizhouense.

show abstract

Function of a hydrophobin in growth and development, nitrogen regulation, and abiotic stress resistance of Ganoderma lucidum

Qiao

Peng

et al. 2023

FEMS Microbiology Letters

View full text Add to dashboard Cite

Fungal hydrophobins have many important physiological functions, such as maintaining hydrophobicity and affecting virulence, growth, and development. In Ganoderma lucidum, the molecular regulation mechanisms of hydrophobins in mushroom are unclear. In this study, we investigated a hydrophobin protein 1 (Hyd1) in G. lucidum, which belongs to the fungal Class I hydrophobins. The hyd1 gene was highly expressed during the formation of primordia, and expression was the lowest in fruiting bodies. Through the construction of hyd1 silenced strains, we found that primordia formation was not initiated in these strains. This finding indicated that Hyd1 played an important role in the development of G. lucidum. Second, AreA, a key transcription factor in nitrogen metabolism, negatively regulated the expression of hyd1. In an areA silenced strain, the expression of hyd1 increased by approximately 14-fold compared with that of the wild-type (WT) strain. Electrophoretic mobility shift assays (EMSA) indicated binding of AreA to the promoter of hyd1. Additionally, expression of hyd1 was determined in the presence of different nitrogen sources. Compared with that in the ammonia nitrogen source, the expression of hyd1 in nitrate nitrogen source significantly increased. Finally, we found that hyd1 plays important roles not only in nitrogen regulation but also in the resistance to other abiotic stresses. After silencing of hyd1, the resistance to heat, cell wall, and salt stresses decreased. Our findings reveal the important roles of Hyd1 in the development and resistance to abiotic stresses in G. lucidum and provide insights into the nitrogen regulation mechanism of hydrophobins in higher basidiomycetes.

show abstract

Comprehensive analysis of the regulatory network of blue‐light‐regulated conidiation and hydrophobin production in Trichoderma guizhouense

Cited by 11 publications

References 58 publications

A Simple and Low-Cost Strategy to Improve Conidial Yield and Stress Resistance of Trichoderma guizhouense through Optimizing Illumination Conditions

A Simple and Low-Cost Strategy to Improve Conidial Yield and Stress Resistance of Trichoderma guizhouense through Optimizing Illumination Conditions

The bZIP transcription factor ATF1 regulates blue light and oxidative stress responses in Trichoderma guizhouense

Function of a hydrophobin in growth and development, nitrogen regulation, and abiotic stress resistance of Ganoderma lucidum

Contact Info

Product

Resources

About