Comprehensive expression analysis of pathogenicity genes in uropathogenic Escherichia coli strains

Paniagua-Contreras, Gloria Luz; Hernández-Jaimes, Tania; Monroy-Pérez, Eric; Vaca-Paniagua, Felipe; Díaz-Velásquez, Clara; Uribe-García, Alina; Vaca, Sergio

doi:10.1016/j.micpath.2016.12.008

Cited by 27 publications

(25 citation statements)

References 28 publications

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“…These results are relevant because they demonstrate, for the first time, the combined transcription of different virulence genes during infection of vaginal cells. The frequencies of transcription of the genes fimH, irp2, iroN, kpsMTII, and cnf1 were higher than the rates previously found by our research group in uropathogenic E. coli strains in vitro, except for the papC gene, which had a similar frequency [35]. It has been demonstrated that the expression of fim in E. coli promotes bacterial colonisation, invasion, and biofilm formation [36], whereas the presence of pap has been associated with pyelonephritis and recurrent urinary tract infections in women [37].…”

Section: Discussionmentioning

confidence: 48%

Virulence gene transcription, phylogroups, and antibiotic resistance of cervico-vaginal pathogenic E. coli in Mexico

et al. 2020

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The pathogenicity of Escherichia coli strains that cause cervico-vaginal infections (CVI) is due to the presence of several virulence genes. The objective of this study was to define the variability regarding the genotype of antibiotic resistance, the transcription profiles of virulence genes after in vitro infection of the vaginal cell line A431 and the phylogroup composition of a group of cervico-vaginal E. coli strains (CVEC). A total of 200 E. coli strains isolated from Mexican women with CVI from two medical units of the Mexican Institute of Social Security were analysed. E. coli strains and antibiotic resistance genes were identified using conventional polymerase chain reaction (PCR), and phylogroups were identified using multiplex PCR. Virulence gene transcription was measured through reverse-transcriptase realtime PCR after infection of the vaginal cell line A431. The most common antibiotic resistance genes among the CVEC strains were aac(3)II, TEM, dfrA1, sul1, and qnrA. The predominant phylogroup was B2. The genes most frequently transcribed in these strains were fimH, papC, irp2, iroN, kpsMTII, cnf1, and ompT, mainly in CVEC strains isolated from chronic and occasional vaginal infections. The strains showed a large diversity of transcription of the virulence genes phenotype and antibiotic resistance genotype, especially in the strains of phylogroups, B2, A, and D. The strains formed 2 large clusters, which contained several subclusters. The genetic diversity of CVEC strains was high. These strains have a large number of transcription patterns of virulence genes, and one-third of them carry three to seven antibiotic resistance genes.

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Section: Discussionmentioning

confidence: 48%

Virulence gene transcription, phylogroups, and antibiotic resistance of cervico-vaginal pathogenic E. coli in Mexico

et al. 2020

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“…P fimbriae, or pilus associated with pyelonephritis (pap type C in the described E. coli), show strong epidemiologic association with pyelonephritis, sepsis, prostatitis (Johnson 2003, Liu et al 2015, which may explain the pyelonephritis without evidence of infection in other sites, mainly urinary bladder. Additionally, the expression of toxin genes, such as cnf1, hlyA and usp identified in this E. coli, may lead to more extensive tissue damage in the host, causing release of host nutrients, disabling of immune effector cells and, consequently, facilitating bacterial dissemination (Paniagua-Contreras et al 2017).…”

Section: Discussionmentioning

confidence: 99%

Bilateral pyelonephritis due to Escherichia coli infection in a captive jaguar (Panthera onca)

et al. 2020

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Extraintestinal pathogenic Escherichia coli (ExPEC) is a highly diverse pathotype of E. coli which colonizes the intestine, and it is considered an important etiological agent associated with bacteremia and other systemic infections, among them urinary tract infection. Retrospective studies evaluating morbidity and mortality of nondomestic felids have demonstrated that urinary tract diseases are among the main causes of death for geriatric animals. Also, mesenchymal neoplasms of the uterus are common in wild felids, and they possess variable morphologic characteristics related to invasiveness and malignancy. This report describes a case of bilateral pyelonephritis due to extraintestinal uropathogenic E. coli infection in a captive jaguar (Panthera onca). The diagnosis was confirmed through pathological, bacterial and immunohistochemical findings. According to molecular analysis, this E. coli strain was classified in the phylogroup F, possessing the following virulence-associated genes: usp, cnf-1, hlyA, papC and sfa. Additionally, this E. coli was highly resistant to β-lactams and first-generation cephalosporin. This jaguar also presented a uterine leiomyoma with distinct distribution, and severe degenerative articular disease, both of them described as frequently seen lesions in geriatric animals from the Panthera genus.

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“…In Mexico, the incidence of UPEC infections is high, reaching 3,000 per 100,000 habitants in 2018 37. We previously characterized UPEC strains from Mexican patients via nongenomic approaches that are limited in terms of gene detection and identified the collective participation of a large repertoire of expressed virulence genes during in vitro infection; therefore, this work represents an advance of this development 16,38…”

Section: Discussionmentioning

confidence: 99%

<p>Whole-genome sequence analysis of multidrug-resistant uropathogenic strains of <em>Escherichia coli</em> from Mexico</p>

Paniagua-Contreras¹,

Monroy-Pérez²,

Díaz-Velásquez³

et al. 2019

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Background : Escherichia coli is the main bacterium associated with urinary tract infections (UTIs), including cystitis and pyelonephritis. Uropathogenic E. coli (UPEC) harbors numerous genes that encode diverse virulence factors contributing to its pathogenicity. The treatment of UTIs has become complicated due to the natural selection of E. coli strains that are multiresistant to several groups of antibiotics regularly used in clinical settings such as hospitals. Genomic reports of the global composition and distribution of the antibiotic resistance and virulence genes of these pathogenic strains are lacking in the Mexican population. Purpose and methods : The aim of this study was to globally characterize the genomes of a group of UPEC strains by massive parallel sequencing to determine the prevalence and distribution of virulence and antibiotic resistance genes associated with different serotypes and phylogenetic groups. Results: The strains exhibited 138-197 virulence genes and 29 antibiotic resistance genes related to antibiotics that are commonly used in clinical practice. Conclusions: These findings are relevant to the definition of new strategies for treating urinary tract infections in public hospitals and private practice. To further define the epidemiological distribution and composition of these virulence and antibiotic resistance genes, larger studies are needed.

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Comprehensive expression analysis of pathogenicity genes in uropathogenic Escherichia coli strains

Cited by 27 publications

References 28 publications

Virulence gene transcription, phylogroups, and antibiotic resistance of cervico-vaginal pathogenic E. coli in Mexico

Virulence gene transcription, phylogroups, and antibiotic resistance of cervico-vaginal pathogenic E. coli in Mexico

Bilateral pyelonephritis due to Escherichia coli infection in a captive jaguar (Panthera onca)

<p>Whole-genome sequence analysis of multidrug-resistant uropathogenic strains of <em>Escherichia coli</em> from Mexico</p>

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