Comprehensive expression analysis of TNF-related apoptosis-inducing ligand and its receptors in colorectal cancer: Correlation with MAPK alterations and clinicopathological associations

Tsikalakis, Spyridon; Chatziandreou, Ilenia; Michalopoulos, Nikolaos V.; Théodoropoulos, Georgios; Sakellariou, Stratigoula; Korkolopoulou, Penelope; Patsouris, Efstratios; Saetta, Angelica A.

doi:10.1016/j.prp.2018.04.019

Cited by 7 publications

(3 citation statements)

References 40 publications

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“…The sequences of the primers for BRAF, KRAS have been published previously (49). For MSI status, a panel of mononucleotide microsatellite markers (BAT25, BAT26, NR21, NR24) was analysed by HRM analysis, performed as reported previously (50,51).…”

Section: Methodsmentioning

confidence: 99%

Microsatellite Instability in Greek Colorectal Carcinoma Patients: Clinicopathological and Molecular Correlations

et al. 2019

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Background/Aim: In the present retrospective study, we assessed the molecular profile and clinicopathological correlations of Greek colorectal carcinoma (CRC) patients. Patients and Methods: Data from 157 CRC patients were collected. High Resolution Melting Analysis and Pyrosequencing/Sanger sequencing were applied to identify KRAS, BRAF, NRAS mutations and microsatellite instability (MSI) status. Immunohistochemistry was performed to characterize the associated Mismatch Repair Protein loss. Statistical calculations were performed using the statistical package SPSS v21.0. Results: KRAS mutations were detected in 39.3% of cases, BRAF in 10.9% and NRAS in 4.9%. MSI status was recognized in 11.5% of CRC patients and was associated with right colon tumors. MSI phenotype was inversely correlated with stage, N status and KRAS mutations and positively correlated with BRAF mutations. Conclusion: MSI positive CRCs in the Greek population are more often right-sided, free of metastasis, KRAS wild type and BRAF mutated. Providing more detailed clinicopathological and molecular data for specific populations will enable better clinical management and individualized therapy in the future.

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Section: Methodsmentioning

confidence: 99%

Microsatellite Instability in Greek Colorectal Carcinoma Patients: Clinicopathological and Molecular Correlations

et al. 2019

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“…Apoptosis is a tightly regulated cellular process and faulty regulation of apoptosis is a hallmark of human cancers. TNF-related apoptosis-inducing ligand (TRAIL) is a type II transmembrane protein and binds its receptors TRAIL-R1 and TRAIL-R2, which in turn recruit downstream adaptor proteins via its intracellular death domain (DD) and activate the intrinsic apoptotic pathway triggering selective neoplastic cell apoptosis [36][37]. This is regulated by non-functional TRAIL receptors, TRAIL-R3 and TRAIL-R4 that are devoid of a cytoplasmic tail or carry a truncated intracellular DD, respectively, and block TRAIL-mediated apoptosis [38][39].…”

Section: Discussionmentioning

confidence: 99%

DPP3 Expression Promotes Cell Proliferation and Migration in Vitro and Tumor Growth in Vivo That Associates with Poor Prognosis of Esophageal Carcinoma

Liu

Abudula

Yang

et al. 2021

Preprint

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Background: Dipeptidyl peptidase III (DPP3) is a zinc-dependent metallopeptidase and elevated in a variety of malignant tumors, but the underlying mechanism is not well understood so far. Here we investigated the association of esophageal carcinogenesis with the regulation of DPP3 expression by tissue-based quantitative analysis and the depletion of DPP3 expression in esophageal cancer cells and xenograft model. Methods: The expression level of DPP3 in esophageal cancer tissues and adjacent normal tissues was detected in 93 cases of tissue biopsies collected from patients diagnosed with esophageal carcinoma by immunohistochemistry. The effect of DPP3 expression on cell proliferation, migration or apoptosis was determined in DPP3-depleted esophageal cancer cells created by infection with the lentivirus containing the shRNA specific to human DPP3 mRNA sequence followed by cytometric detection using celigo cell count assay, flow cytometry, wound-healing assay and trans-well assay as well as chip screening with a Human Apoptosis Antibody Array kit, which enables the quantitative detection of 43 apoptosis-related genes. A xenograft model was applied to the detection of tumor growth and invasion of DPP3-depleted cancer cells in nude mice.Results: DPP3 expression was elevated in esophageal cancer tissues compared with adjacent non-tumor tissues (normal controls) with statistical significance (P<0.05), and associated with poor prognosis of esophageal carcinoma. The DPP3-depletion resulted in a reduced cell proliferation and migration and enhanced cell-cycle arrest and apoptosis of esophageal cancer cells, and lead to the inhibition of tumor growth and invasion in xenograft model. In addition, DPP3-depletion was associated with the upregulation of pro-apoptotic proteins and the downregulation of anti-apoptotic proteins.Conclusions: These findings suggest that DPP3 may promote cell proliferation, migration and survival of esophageal cancer cells in vitro, and tumor growth and invasion of esophageal carcinoma in vivo and this might serve as a molecular target for tumor therapy.

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“…The extracted DNA was quantitated on a Picodrop microliter spectrophotometer. Samples were screened in duplicates for mutations of KRAS, NRAS , and BRAF , using a real-time polymerase chain reaction approach followed by high-resolution melting analysis on a Light Cycler 480 (Roche Diagnostics, GmbH, Germany) 25. Polymerase chain reaction products positive by high-resolution melting analysis were purified and subjected to Sanger sequencing and/or pyrosequencing.…”

Section: Methodsmentioning

confidence: 99%

Autophagy-related Proteins as a Prognostic Factor of Patients With Colorectal Cancer

Koustas

Sarantis

Theoharis³

et al. 2019

American Journal of Clinical Oncology

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Comprehensive expression analysis of TNF-related apoptosis-inducing ligand and its receptors in colorectal cancer: Correlation with MAPK alterations and clinicopathological associations

Cited by 7 publications

References 40 publications

Microsatellite Instability in Greek Colorectal Carcinoma Patients: Clinicopathological and Molecular Correlations

Microsatellite Instability in Greek Colorectal Carcinoma Patients: Clinicopathological and Molecular Correlations

DPP3 Expression Promotes Cell Proliferation and Migration in Vitro and Tumor Growth in Vivo That Associates with Poor Prognosis of Esophageal Carcinoma

Autophagy-related Proteins as a Prognostic Factor of Patients With Colorectal Cancer

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