Comprehensive genomic analysis of the TYROSINE AMINOTRANSFERASE (TAT) genes in apple (Malus domestica) allows the identification of MdTAT2 conferring tolerance to drought and osmotic stresses in plants

Wang, Haibo; Dong, Qinglong; Duan, Dingyue; Zhao, Shuang; Li, Mingjun; Nocker, Steve van; Ma, Fengwang; Mao, Ke

doi:10.1016/j.plaphy.2018.10.033

Cited by 20 publications

(19 citation statements)

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“…Tyrosine aminotransferase (TAT, EC 2.6.1.5) is the rst key enzyme that catalyzes the reversible interconversion of tyrosine and 4-hydroxyphenylpyruvate in the tyrosine-derived pathway for syntheses of important secondary metabolites and compounds. Moreover, many TAT genes have been cloned from different plant species (Ipson et al, 2019;Wang et al, 2018;Xu et al, 2020). Now, there are several reports about TAT overexpressions in potato for cellulose biosynthesis and Perilla frutescens for rosmarinic acid (Li 2007;Lu et al, 2013), but there is not any report about TAT overexpression for acteoside biosynthesis in Rehmannia glutinosa.…”

Section: Discussionmentioning

confidence: 99%

“…Tyrosine aminotransferase (TAT) is the rst key enzyme that catalyzes the reversible interconversion of tyrosine and 4-hydroxyphenylpyruvate in the tyrosine-derived branch pathway of acteoside biosynthesis (Zhou et al, 2020). Plant TAT genes have been proposed to be important in response to abiotic stress, including drought and osmotic stresses (Wang et al, 2018) and oxidative stress (Ipson et al, 2019), and to take part in rosmarinic acid synthesis (Lu et al, 2013). In addition, the cloning of TAT genes has been reported from some plant species in NCBI.…”

Section: Introductionmentioning

confidence: 99%

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Molecular Cloning, Bioinformatics Analysis and Overexpression of the Tyrosine Aminotransferase Gene in Rehmannia Glutinosa

Zhou

Zhu

Zhang

et al. 2021

Preprint

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Rehmannia glutinosa is an important medicinal plant producing many bioactive compounds such as catalpol, acteoside and so on. Tyrosine aminotransferase (TAT) is the first key enzyme that catalyzes the reversible interconversion of tyrosine and 4-hydroxyphenylpyruvate in the tyrosine-derived branch pathway of acteoside biosynthesis. To confirm its role for acteoside accumulation, we isolated a full-length cDNA from Rehmannia glutinosa Libosch. Sequence analysis indicated that it contained a 1266 bp open reading frame, encoding a TAT of 421 amino acid residues. Multiple sequence alignment revealed that the homology of RgTAT amino acid sequence to that of Sesamum indicum(XP_011100354.1) was the highest (89.94%). Evolutionary tree showed that Sesamum indicum TAT and RgTAT were grouped together. Quantitative real-time PCR analysis indicated that the expression of RgTAT in leaves was much higher than in roots and stems,and that the expression levels of RgTAT in the tuberous roots, stems and leaves of high-acteoside cultivar BJ-3 were higher than in that of low-acteoside cultivar Wen85-5. A plant expression vector was constructed containing the RgTAT and hygromycin resistance gene (Hyg). Transgenic Rehmannia glutinosa Libosch overexpressing RgTAT was obtained via an Agrobacterium tumefaciens-mediated transformation system, in which Hyg expression was confirmed by PCR. RgTAT expression in transgenic plantlets measured by real-time quantitative PCR was 7.72 ± 0.17 times greater than its expression in the untransformed plantlets. Moreover, HPLC analysis indicated that enhanced RgTAT expression corresponded to significantly increased acteoside for transgenic plantlets. Our results elucidate the role of RgTAT in the acteoside biosynthesis in Rehmannia glutinosa.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Molecular Cloning, Bioinformatics Analysis and Overexpression of the Tyrosine Aminotransferase Gene in Rehmannia Glutinosa

Zhou

Zhu

Zhang

et al. 2021

Preprint

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“…The primers used for constructing these vectors are shown in Supplementary Table S9. Wild-type (WT) “Orin” apple calli were transformed by Agrobacterium -mediated transformation (strain EHA105) 45 . Transgenic calli harboring 35S:MIR156p or 35S:MIRn-249 were screened on MS agar medium (1.5 mg/L 2,4-dichlorophenoxyacetic acid (2,4-D), 0.4 mg/L 6-BA, 30 g/L sucrose, and 8 g/L agar) containing 50 mg/L kanamycin and 250 mg/L cefotaxime.…”

Section: Methodsmentioning

confidence: 99%

Genome-wide identification of drought-responsive microRNAs in two sets of Malus from interspecific hybrid progenies

Niu

Jiang

et al. 2019

Hortic Res

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Drought stress can negatively impact apple fruit quality and yield. Apple microRNAs (miRNAs) participate in apple tree and fruit development, as well as in biotic stress tolerance; however, it is largely unknown whether these molecules are involved in the drought response. To identify drought-responsive miRNAs in Malus , we first examined the drought stress tolerance of ten F 1 progenies of R3 ( M . × domestica ) × M. sieversii . We performed Illumina sequencing on pooled total RNA from both drought-tolerant and drought-sensitive plants. The sequencing results identified a total of 206 known miRNAs and 253 candidate novel miRNAs from drought-tolerant plants and drought-sensitive plants under control or drought conditions. We identified 67 miRNAs that were differentially expressed in drought-tolerant plants compared with drought-sensitive plants under drought conditions. Under drought stress, 61 and 35 miRNAs were differentially expressed in drought-tolerant and drought-sensitive plants, respectively. We determined the expression levels of seven out of eight miRNAs by stem-loop qPCR analysis. We also predicted the target genes of all differentially expressed miRNAs and identified the expression of some genes. Gene Ontology analyses indicated that the target genes were mainly involved in stimulus response and cellular and metabolic processes. Finally, we confirmed roles of two miRNAs in apple response to mannitol. Our results reveal candidate miRNAs and their associated mRNAs that could be targeted for improving drought tolerance in Malus species, thus providing a foundation for understanding the molecular networks involved in the response of apple trees to drought stress.

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“…L-tyrosine is required for protein synthesis and also serves as a precursor for several plant metabolites, including alkaloids, phenylquinones, and cyanoglycosides [115]. Tyrosine aminotransferase (TAT) is the rst key enzyme for the synthesis of important secondary metabolites [116] and has a proposed role in abiotic stress response [117]. It catalyzes the reversible conversion of tyrosine and 4-hydroxyphenylpyruvate in the tyrosine-derived pathway.…”

Section: Genes Involved Insecondarymetabolismmentioning

confidence: 99%

Comparative analysis of alfalfa (Medicago sativa L.) seedling transcriptomes reveals genotype-specific drought tolerance mechanisms

Wang

et al. 2021

Plant Physiology and Biochemistry

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BackgroundDrought is one of the main abiotic factors that affect alfalfa yield. The identi cation of genes that control this complex trait can provide important insights for alfalfa breeding. However, little is known about how alfalfa responds and adapts to drought stress, particularly in cultivars of differing drought tolerance. ResultsIn this study, the drought-tolerant cultivar Dryland 'DT' and the drought-sensitive cultivar WL343HQ 'DS' were used to characterize leaf and root physiological responses and transcriptional changes in response to water de cit. Under drought stress, Dryland roots (DTR) showed more differentially expressed genes than WL343HQ roots (DSR), whereas WL343HQ leaves (DSL) showed more differentially expressed genes than Dryland leaves (DTL). Many of these genes were involved in stress-related pathways, carbohydrate metabolism, and lignin and wax biosynthesis, which may have improved the drought tolerance of alfalfa. We also observed that several genes related to ABA metabolism, root elongation, peroxidase activity, cell membrane stability, ubiquitination, and genetic processing responded to drought stress in alfalfa. We highlighted several candidate genes, including sucrose synthase, xylan 1,4-betaxylosidase, primary-amine oxidase, and alcohol-forming fatty acyl-CoA reductase, for future studies on drought stress resistance in alfalfa and other plant species. ConclusionsIn summary, our results reveal the unique drought adaptation and resistance characteristics of two alfalfa genotypes. These ndings, which may be valuable for drought resistance breeding, warrant further gene functional analysis to augment currently available information and to clarify the drought stress regulatory mechanisms of alfalfa and other plants. HighlightsWe assembled leaf and root transcriptomes of drought-tolerant and drought-sensitive alfalfa cultivars under control and drought conditions.Under drought, the drought-tolerant cultivar exhibited more differentially expressed metabolites than the drought-sensitive cultivar.Genes associated with carbon metabolism, amino acid metabolism, hormones, and secondary metabolites were differentially expressed under drought stress.The drought-tolerant cultivar exhibited higher expression of genes encoding sucrose synthase, xylan 1,4-beta-xylosidase, primary-amine oxidase, and alcohol-forming fatty acyl-CoA.

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Comprehensive genomic analysis of the TYROSINE AMINOTRANSFERASE (TAT) genes in apple (Malus domestica) allows the identification of MdTAT2 conferring tolerance to drought and osmotic stresses in plants

Cited by 20 publications

References 42 publications

Molecular Cloning, Bioinformatics Analysis and Overexpression of the Tyrosine Aminotransferase Gene in Rehmannia Glutinosa

Molecular Cloning, Bioinformatics Analysis and Overexpression of the Tyrosine Aminotransferase Gene in Rehmannia Glutinosa

Genome-wide identification of drought-responsive microRNAs in two sets of Malus from interspecific hybrid progenies

Comparative analysis of alfalfa (Medicago sativa L.) seedling transcriptomes reveals genotype-specific drought tolerance mechanisms

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