Comprehensive immune complexome analysis detects disease-specific immune complex antigens in seminal plasma and follicular fluids derived from infertile men and women

Murakami, Naoko; Kitajima, Michio; Ohyama, Kaname; Aibara, Nozomi; Taniguchi, Koichi; Wei, Mian; Kitajima, Yuriko; Miura, Kiyonori; Masuzaki, Hiroaki

doi:10.1016/j.cca.2019.05.031

Cited by 8 publications

(4 citation statements)

References 48 publications

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“…However, in the present study, this analysis was modified to evaluate the ICs in other fluid samples. In fact, we identified disease-specific IC-antigen from cerebrospinal fluid in patients with central nervous system autoimmune diseases and from follicular fluid in infertile patients (19,20). One of most important results in the present study is that IC-ceruloplasmin was the most useful BC-related urine marker because its positive rate in BC was higher than that in all non- tumoral conditions and was also significantly associated with grade, T stage, invasive potential, and urinary tract recurrence.…”

Section: Discussionmentioning

confidence: 52%

Detection of Novel Urine Markers Using Immune Complexome Analysis in Bladder Cancer Patients: A Preliminary Study

Aibara

Miyata

Araki

et al. 2021

In Vivo

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Background/Aim: Little is known on urine biomarkers that are associated with malignant behavior in patients with bladder cancer (BC). Our aim was to identify BC-related factors in urine samples using our original method "immune complexome analysis", based on detecting the immune complex (IC). Patients and Methods: Immune complexome analysis was performed using urine samples from 97 BC patients, including 67 with non-muscle invasive BC (NMIBC). Results: Eight IC-antigens were recognized as candidates for BC-related factors from 20,165 proteins. ICserum albumin, -fibrinogen γ chain, -hemoglobin subunit α, -hemoglobin subunit β, -ceruloplasmin, and fibrinogen β chain were significantly associated with either pathological features and/or outcome. IC-ceruloplasmin was most widely associated with pathological features in all BC patients and lamina propria invasion and urinary tract recurrence in NMIBC. Conclusion: Based on detection of IC-antigens it was demonstrated that six IC-antigens, especially ICceruloplasmin, are potential urine biomarkers in BC.

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Section: Discussionmentioning

confidence: 52%

Detection of Novel Urine Markers Using Immune Complexome Analysis in Bladder Cancer Patients: A Preliminary Study

Aibara

Miyata

Araki

et al. 2021

In Vivo

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“…found 4 disease-specific antigens in oligozoospermia patients and 5 disease-specific antigens in asthenospermia patients. The formation and deposition of immune complexes stimulate inflammation through the action of the complement system, leading to reproductive organ fibrosis and loss of related protein function, thus leading to spermatogenic dysfunction ( 120 ).…”

Section: Oligozoospermia Asthenospermia Azoospermiamentioning

confidence: 99%

Microbiology and immune mechanisms associated with male infertility

Chen

Fang

et al. 2023

Front. Immunol.

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Up to 50% of infertility is caused by the male side. Varicocele, orchitis, prostatitis, oligospermia, asthenospermia, and azoospermia are common causes of impaired male reproductive function and male infertility. In recent years, more and more studies have shown that microorganisms play an increasingly important role in the occurrence of these diseases. This review will discuss the microbiological changes associated with male infertility from the perspective of etiology, and how microorganisms affect the normal function of the male reproductive system through immune mechanisms. Linking male infertility with microbiome and immunomics can help us recognize the immune response under different disease states, providing more targeted immune target therapy for these diseases, and even the possibility of combined immunotherapy and microbial therapy for male infertility.

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“…We previously developed an ‘immune complexome analysis’ capable of identifying specific antigens in ICs in biological fluids. The method uses IC‐capturing beads and nano‐liquid chromatography–tandem mass spectrometry (nano‐LC‐MS/MS) to comprehensively identify and profile IC‐antigens [19–23]. To confirm the presence of ICs formed by AMA‐antigens in PBC sera, we analyzed sera from patients with PBC as well as four other typical autoimmune diseases (SS, SLE, RA and SSc) using immune complexome analysis.…”

Section: Introductionmentioning

confidence: 99%

Investigation of immune complexes formed by mitochondrial antigens containing a new lipoylated site in sera of primary biliary cholangitis patients

Aibara

Ohyama

Nakamura

et al. 2021

Clinical and Experimental Immunology

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Summary Primary biliary cholangitis (PBC) is characterized by the presence of serum anti‐mitochondrial autoantibodies (AMAs). To date, four antigens among the 2‐oxo‐acid dehydrogenase complex family, which commonly have lipoyl domains as an epitope, have been identified as AMA‐corresponding antigens (AMA‐antigens). It has recently been reported that AMAs react more strongly with certain chemically modified mimics than with the native lipoyl domains in AMA‐antigens. Moreover, high concentrations of circulating immune complexes (ICs) in PBC patients have been reported. However, the existence of ICs formed by AMAs and their antigens has not been reported to date. We hypothesized that AMAs and their antigens formed ICs in PBC sera, and analyzed sera of PBC and four autoimmune diseases (Sjögren's syndrome, systemic lupus erythematosus, systemic scleroderma, and rheumatoid arthritis) using immune complexome analysis, in which ICs are separated from serum and are identified by nano‐liquid chromatography‐tandem mass spectrometry. To correctly assign MS/MS spectra to peptide sequences, we used a protein‐search algorithm that including lipoylation and certain xenobiotic modifications. We found three AMA‐antigens, the E2 subunit of the pyruvate dehydrogenase complex (PDC‐E2), the E2 subunit of the 2‐oxo‐glutarate dehydrogenase complex (OGDC‐E2) and dihydrolipoamide dehydrogenase binding protein (E3BP), by detecting peptides containing lipoylation and xenobiotic modifications from PBC sera. Although the lipoylated sites of these peptides were different from the well‐known sites, abnormal lipoylation and xenobiotic modification may lead to production of AMAs and the formation ICs. Further investigation of the lipoylated sites, xenobiotic modifications, and IC formation will lead to deepen our understanding of PBC pathogenesis.

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Comprehensive immune complexome analysis detects disease-specific immune complex antigens in seminal plasma and follicular fluids derived from infertile men and women

Cited by 8 publications

References 48 publications

Detection of Novel Urine Markers Using Immune Complexome Analysis in Bladder Cancer Patients: A Preliminary Study

Detection of Novel Urine Markers Using Immune Complexome Analysis in Bladder Cancer Patients: A Preliminary Study

Microbiology and immune mechanisms associated with male infertility

Investigation of immune complexes formed by mitochondrial antigens containing a new lipoylated site in sera of primary biliary cholangitis patients

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