Comprehensive Invitro Evaluation of Pharmacological Activities of Selected Plant Extracts and Gc-MS Profiling of Flacourtia Jangomas Flower Extract

Abstract

Objective: Continued exploration and bio-evaluation of plants hold good as there are an increasing demand and recognition of natural products in disease management. The aim of this study was to investigate the antioxidant, cytotoxic, and antiproliferative activity of methanolic extracts of different parts of six plants. Six plants -Couroupita guianensis, Flacourtia jangomas, Lucuma nervosa, Euphorbia milii, Acalypha hispida, and Hydnocarpus pentandra -were chosen for this study. Methods:The plant parts were extracted with methanol and screened for 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2′-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) radical scavenging abilities. The cytotoxic activity of the extracts was investigated on SCC9 and Calu6 through MTT assay, and cell cycle was analyzed by flow cytometry to determine the antiproliferative activity of the extracts. The flower extract of F. jangomas was further subjected to gas chromatography-mass spectrometry (GC-MS) analysis for purification of the compounds of interest. A twoway ANOVA was done to estimate the effect of the extract between samples remembered at p<0.05 level.Results: Among all the plant extracts, the extract of F. jangomas (flower) showed significant antioxidant potential with IC 50 values of 11.16±0.54 µg/ml and 12.34±0.37 µg/ml for DPPH and ABTS assays, respectively. GC-MS analysis of the extract revealed the presence of 21 phytocompounds. MTT assay revealed that this extract had promising cytotoxic activity against the two cancer cell lines, Calu6 and SCC9 with IC 50 values of 43.57±0.04 µg/ml and 53.42±0.15 µg/ml, respectively. The extract treatment caused significant arrest in G 2 M phase of cell cycle. Conclusion:F. jangomas flower extract displayed significant antioxidant and antiproliferative activity and can be considered as a potential source of anticancer compounds.

Phytochemical analysis, Antimicrobial, insecticidal and antiradical activity of <em>Hydnocarpus pentandra</em> (Buch.-Ham.) Oken

“…A scavenging of >50% was observed at extract concentration 6.25µg/ml and higher ( Figure 2). Based on the IC 50 values, the ABTS radical scavenging potential of leaf extract (6.03µg/ml) in this study was higher than that of the result of ABTS radical scavenging potential by leaf extract (>30µg/ml) of the study carried out by George et al [28]. In the present study, the scavenging potential of leaf extract of H. pentandra observed was lower than that of ascorbic acid (IC 50 value 2.48µg/ml).…”

“…The seed and seed oil of H. pentandra are used traditionally to treat ailments such as leprosy, skin diseases, eczema, dermatitis, tubercular laryngitis, chronic ulcers, dyspepsia, flatulence and verminosis by local people of sacred groves of Trissur district, Kerala, India [34]. The plant is reported to exhibit bioactivities such as larvicidal [19], antioxidant [35], antibacterial [36] and anticancer [28] [37] activity. The present study was performed to investigate antimicrobial, insecticidal and antiradical potential of methanol extract of leaves of H. pentandra.…”

Anti-Tubercular Activity of Extract and Coumpounds of Noni (Morinda Citrifolia Linn)

“…Phenolic compounds contained in the noni fruit ranged from 5.94 to 36.52 g/100 g of dry material [24]. Solvent organic extracts contain a mixture of secondary metabolites including alkaloids, flavonoids, terpenoids, and other phenolic compounds; these molecules are associated with the defense mechanisms of plants by their repellent or attractive properties, protection against biotic and abiotic stresses, and maintenance of structural integrity of plants [15,28], and the class of natural compounds possessing a wide range of pharmacological activities [29,30].…”

Ethnomedicinal, Phytochemical and Pharmacological Aspects of Flacourtia Jangomas: A Review

“…Methanol extract of fruits contain a very good amount of phenolics also showed strong radical scavenging activity [61]. Antioxidant efficacy of different parts of the plant was studied using DPPH and ABTS radical scavenging assay; of which the flower extract of F. jangomas showed significant antioxidant potential with IC50 values of 11.16±0.54 μg/ml and 12.34±0.37 μg/ml for DPPH and ABTS assays [62].…”

“…Methanolic extract of flower showed promising cytotoxic activity against the two cancer cell lines, Calu6 and SCC9 with IC50 values of 43.57±0.04 μg/ml and 53.42±0.15 μg/ml, respectively. The antiproliferative activity of extracts investigated by analyzing cell cycle through flow cytometry revealed that treatment with methanol extract of flower caused significant arrest in G2M phase of cell cycle [62].…”