Comprehensive List of Lectins: Origins, Natures, and Carbohydrate Specificities

Kobayashi, Yuka; Tateno, Hiroaki; Ogawa, Haruko; Yamamoto, Kazuo; Hirabayashi, Jun

doi:10.1007/978-1-4939-1292-6_45

Cited by 38 publications

(23 citation statements)

References 228 publications

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“…In a similar manner, RCA120 appears to recognize D-FucNAc even though GalNAc is described as only a low-affinity ligand (75). UEA I is known to bind Fuc (73) and also seems to tolerate substitutions at C2, as the MPS library array data reveal interactions with FucNAc terminating structures 77, 81, and 103 (SI Appendix, Fig. S2).…”

Section: Discussionmentioning

confidence: 91%

Microbe-focused glycan array screening platform

Geissner

Reinhardt

Rademacher

et al. 2019

Proc. Natl. Acad. Sci. U.S.A.

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Interactions between glycans and glycan binding proteins are essential for numerous processes in all kingdoms of life. Glycan microarrays are an excellent tool to examine protein–glycan interactions. Here, we present a microbe-focused glycan microarray platform based on oligosaccharides obtained by chemical synthesis. Glycans were generated by combining different carbohydrate synthesis approaches including automated glycan assembly, solution-phase synthesis, and chemoenzymatic methods. The current library of more than 300 glycans is as diverse as the mammalian glycan array from the Consortium for Functional Glycomics and, due to its microbial focus, highly complementary. This glycan platform is essential for the characterization of various classes of glycan binding proteins. Applications of this glycan array platform are highlighted by the characterization of innate immune receptors and bacterial virulence factors as well as the analysis of human humoral immunity to pathogenic glycans.

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Section: Discussionmentioning

confidence: 91%

Microbe-focused glycan array screening platform

Geissner

Reinhardt

Rademacher

et al. 2019

Proc. Natl. Acad. Sci. U.S.A.

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“…Some lectins can interact only with mannose or glucose residues and others only with galactose. There are other lectins specific to fucose, sialic acid, and other monosaccharides (Kobayashi et al, 2014;Nagdas et al, 2014).…”

Section: Resultsmentioning

confidence: 99%

Distribution Profile and Function of Carbohydrate Residues in Testes of Immature and Mature Sunda Porcupine (Hystrix javanica).

Budipitojo¹,

Padeta²,

Yulianti³

et al. 2020

jwpr

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The population of Sunda porcupine (Hystrix javanica) declines each year since it is rarely found in nature. The present study aimed to obtain information about the distribution of carbohydrate residues contained in immature and mature of Sunda porcupine's testes and to discuss its relevant functions. This study used six testes obtained from four immature and two mature Sunda porcupine originated from Ngawi Regency, East Java Province, Indonesia. Testis tissues were stained with hematoxylin and eosin and lectin histochemistry of Lens culinaris agglutinin (LCA), Phaseolus vulgaris leucoagglutinin (PHA-L), Pisum sativum agglutinin (PSA), Sophora japonica agglutinin (SJA), and Wheat germ agglutinin (WGA). Data were analyzed with descriptive and semi-quantitative method. Lectin histochemical staining with LCA, PHA-L, SJA, PSA, and WGA indicated the presence of alpha-D-mannose and alpha-D-glucose, N-acetylgalactosamine, mannose, and N-acetylglucosamine residues in the immature and mature testes with weak to very strong intensity. In the immature testes of Sunda porcupine, there was positive reactivity with PHA-L for Leydig and Sertoli cells, N-acetylgalactosamine may play an important role in the development and maturation of Leydig and Sertoli cells. Mature testes showed a strong positive reaction to the LCA, SJA, PSA, and WGA which indicated the significant roles of alpha-D-mannose and alpha-D-glucose, N-acetylgalactosamine, mannose, and N-acetylglucosamine residues on the maturation process of early spermatid to the late spermatid. These results can be used as basic data to be implemented in the conservation efforts of Sunda porcupine.

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“…Afterwards, experiments were also carried out with groups A and O citrated human whole blood (from Innovative Research). It should be underlined that since HPA labeling does not require the presence of divalent cations (Kobayashi et al, 2014), blood treated to prevent its coagulation (e.g., citrate- or EDTA-treated) can be used.…”

Section: Methodsmentioning

confidence: 99%

Fluorescence Imaging of Streptococcus pneumoniae with the Helix pomatia agglutinin (HPA) As a Potential, Rapid Diagnostic Tool

Domenech

Garcı́a

2017

Front. Microbiol.

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Streptococcus pneumoniae is a common human pathogen and a major causal agent of life-threatening infections that can either be respiratory or non-respiratory. It is well known that the Helix pomatia (edible snail) agglutinin (HPA) lectin shows specificity for terminal αGalNAc residues present, among other locations, in the Forssman pentasaccharide (αGalNAc1→3βGalNAc1→3αGal1→4βGal1→4βGlc). Based on experiments involving choline-independent mutants and different growth conditions, we propose here that HPA recognizes the αGalNAc terminal residues of the cell wall teichoic and lipoteichoic acids of S. pneumoniae. In addition, experimental evidence showing that pneumococci can be specifically labeled with HPA when growing as planktonic cultures as well as in mixed biofilms of S. pneumoniae and Haemophilus influenzae has been obtained. It should be underlined that pneumococci were HPA-labeled despite of the presence of a capsule. Although some non-pneumococcal species also bind the agglutinin, HPA-binding combined with fluorescence microscopy constitutes a suitable tool for identifying S. pneumoniae and, if used in conjunction with Gram staining and/or other suitable technique like antigen detection, it may potentially facilitate a fast and accurate diagnosis of pneumococcal infections.

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Comprehensive List of Lectins: Origins, Natures, and Carbohydrate Specificities

Cited by 38 publications

References 228 publications

Microbe-focused glycan array screening platform

Microbe-focused glycan array screening platform

Distribution Profile and Function of Carbohydrate Residues in Testes of Immature and Mature Sunda Porcupine (Hystrix javanica).

Fluorescence Imaging of Streptococcus pneumoniae with the Helix pomatia agglutinin (HPA) As a Potential, Rapid Diagnostic Tool

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