Comprehensive network map of transcriptional activation of chicken type I IFNs and IFN-stimulated genes

Anjum, Faisal Rasheed; Rahman, Sajjad Ur; Aslam, Muhammad; Qureshi, Anas Sarwar

doi:10.1016/j.cimid.2019.101407

Cited by 8 publications

(9 citation statements)

References 127 publications

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“…Chicken type I IFNs (chIFNs) represent the key constituent of the chicken innate immune system and protect the host from the invading viral pathogens. Three subtypes of chicken type I IFNs are chIFN-α, chIFN-β, and chIFN-κ ( 14 , 15 ). Although the three members of the chicken type I IFN family are structurally diverse, they nevertheless bind to the same complex of heterodimer receptors composed of IFNAR1 and IFNAR2 chains ( 14 , 16 ).…”

Section: Introductionmentioning

confidence: 99%

“…Three subtypes of chicken type I IFNs are chIFN-α, chIFN-β, and chIFN-κ ( 14 , 15 ). Although the three members of the chicken type I IFN family are structurally diverse, they nevertheless bind to the same complex of heterodimer receptors composed of IFNAR1 and IFNAR2 chains ( 14 , 16 ). Binding of type I IFNs to their corresponding receptor complex initiates a cascade of intracellular events, which ultimately results in the activation of several hundred IFN-stimulated genes (ISGs) via the JAK-STAT pathway ( 16 , 17 ).…”

Section: Introductionmentioning

confidence: 99%

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Comparative Study of Protection against Newcastle Disease in Young Broilers Administered Natural Chicken Alpha Interferon via Oral and Intramuscular Routes

Anjum

Rahman

Aslam

et al. 2020

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Despite extensive vaccination approaches, Newcastle disease (ND) remains a permanent threat to the poultry industry worldwide. Besides vaccination, there is a burgeoning demand for new antivirals for use in interventions to control ND. One strategy is to strengthen the host innate immunity via host-derived innate immune proteins. Type I interferons define one of the first lines of innate immune defense against viral infections. Chicken interferon alpha (chIFN-α) is one of the potent cytokines that trigger antiviral responses. In the current study, we investigated the therapeutic effect of natural chIFN-α administered via oral and intramuscular (i.m.) routes against ND in broiler chickens. Our results showed that the level of protection against ND in response to chIFN-α therapy was dependent on the route and dose of IFN administration. A better therapeutic effect was observed in chickens treated with chIFN-α via the oral route than in those treated via the i.m. route. Regardless of the administration route, double-dose chIFN-α (2,000-U) treatments provided better protection than single-dose (1,000-U) treatments. However, complete protection against ND was achieved in birds treated with repeated doses of chIFN-α via the oral route. Histopathology of trachea, proventriculus, spleen, and liver showed a significant improvement in ND-induced degenerative changes in double-dose IFN-treatment groups compared to single-dose groups. Results of the hemagglutination test demonstrated a decrease in ND virus (NDV) titer in IFN-treated groups. Also, double doses of chIFN-α via oral route resulted in early recovery in weight gain. We propose that chIFN-α therapy via oral route could be an important therapeutic tool to control NDV infection in chicken. IMPORTANCE Newcastle disease (ND) is an economically important contagious disease of wild and domestic birds worldwide. The disease causes severe economic losses in terms of production due to high mortality and morbidity in nonvaccinated chickens. Despite extensive vaccination approaches, Newcastle disease (ND) remains a permanent threat to the poultry industry worldwide. In the current study, we used natural chicken IFN-α as an innate immune modulator to counteract ND in chickens. We report that chIFN-α is effective in protecting the chickens against ND and also prevents shedding of the virus, which can then prevent further spread of the disease. We propose that in addition to vaccination, chIFN-α therapy could be an effective option for controlling ND in areas of endemicity.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Comparative Study of Protection against Newcastle Disease in Young Broilers Administered Natural Chicken Alpha Interferon via Oral and Intramuscular Routes

Anjum

Rahman

Aslam

et al. 2020

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“…https://doi.org/10.17221/106/2020-VETMED types of type I IFNs have been discovered in chickens named as chicken IFN-α (chIFN-α), chicken IFN-β (chIFN-β) and chicken IFN-κ (chIFN-κ) (Santhakumar et al 2017a;Anjum et al 2020a). Chicken type I IFNs are primarily produced by fibroblast cells in response to viruses (Santhakumar et al 2017b).…”

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confidence: 99%

“…In general, viral nucleic acids (RNA/ DNA) are identified by the chicken pattern recognition receptors (i.e., chicken MDA5, chicken TLRs or chicken DNA sensors) and result in the downstream intracellular signalling and activation of transcription factors such as IFN regulatory factors 7 (IRF7), NF-κB, and AP-1. The activation of IRF7, NF-κB, and AP-1 results in their intranuclear localisation followed by the induction of type I IFNs from host cells (Anjum et al 2020a). Despite the structural diversity, all three subtypes of chicken type I IFNs (chIFN-α, chIFN-β, and chIFN-κ) bind to the same complex of heterodimer receptors comprised of IFNAR1 and IFNAR2 chains (Santhakumar et al 2019;Anjum et al 2020a).…”

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confidence: 99%

“…The activation of IRF7, NF-κB, and AP-1 results in their intranuclear localisation followed by the induction of type I IFNs from host cells (Anjum et al 2020a). Despite the structural diversity, all three subtypes of chicken type I IFNs (chIFN-α, chIFN-β, and chIFN-κ) bind to the same complex of heterodimer receptors comprised of IFNAR1 and IFNAR2 chains (Santhakumar et al 2019;Anjum et al 2020a). Type I IFNs binding to their receptors activate the JAK-STAT pathway followed by the activation and intranuclear localisation of the IFN-stimulated gene factor 3 (ISGF3) transcription factor complex.…”

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Antiviral potential and stability analysis of chicken interferon-α produced by Newcastle disease virus in chicken embryo fibroblast cells

Anjum¹,

Rahman²,

Aslam³

et al. 2021

Vet. Med. - Czech

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Chicken interferon-α (chIFN-α) is an important antiviral cytokine and represents one of the first lines of the chicken’s innate immune system. The current study is the first-ever report of chicken IFN (chIFN) production in Pakistan. In this study, we have used live and UV-irradiated Newcastle disease virus (NDV) to induce the expression of chIFN-α in chicken embryo fibroblast (CEF) cells. ChIFN-α was partially purified in a two-step protocol; ultracentrifugation followed by treatment with anti-chIFN-β antibodies. The purified chIFN-α was ana-lysed via sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and the in vitro antiviral potential of chIFN-α was determined against the H9N2 avian influenza virus (AIV) via a cytopathic inhibition assay. The relative mRNA level of the IFN-stimulated genes (ISGs) in the IFN-stimulated CEF cells was measured at various time intervals by a quantitative polymerase chain reaction (qPCR). The stability of natural chIFN-α to the temperature, pH, and ultraviolet (UV) light was also determined. The in vivo therapeutic potential of chIFN-α was determined in 7-day-old broiler chickens challenged with AIV. We found that a higher chIFN-α expression level was induced by the UV-irradiated NDV in the CEF cells as compared to the live NDV. The UV-irradiated NDV induced the maximum IFN production in the CEF cells at 24 h post-infection. Two bands of 21 kDa on SDS-PAGE confirmed the presence of the chIFN-α protein. The cytopathic inhibition assay indicated the strong antiviral activity of chIFN-α against AIV. Our results of the stability analysis showed that chIFN-α was stable at a wide range of temperatures and pH levels. However, a little exposure to UV-light resulted in a significant loss of antiviral activity. We also observed that the antiviral activity of chIFN-α is related to the expression levels of the antiviral ISGs. The results of the in vivo study showed that the chIFN-α therapy via the oral route resulted in a significant improvement in the tracheal pathology of chickens challenged with AIV. In conclusion, we suggest that chIFN-α could be an important therapeutic tool to control avian influenza infection in poultry.

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IBV QX affects the antigen presentation function of BMDCs through nonstructural protein16

Wu¹,

Li²,

Zhao³

et al. 2023

Poultry Science

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Comprehensive network map of transcriptional activation of chicken type I IFNs and IFN-stimulated genes

Cited by 8 publications

References 127 publications

Comparative Study of Protection against Newcastle Disease in Young Broilers Administered Natural Chicken Alpha Interferon via Oral and Intramuscular Routes

Comparative Study of Protection against Newcastle Disease in Young Broilers Administered Natural Chicken Alpha Interferon via Oral and Intramuscular Routes

Antiviral potential and stability analysis of chicken interferon-α produced by Newcastle disease virus in chicken embryo fibroblast cells

IBV QX affects the antigen presentation function of BMDCs through nonstructural protein16

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Comprehensive network map of transcriptional activation of chicken type I IFNs and IFN-stimulated genes

Cited by 8 publications

References 127 publications

Comparative Study of Protection against Newcastle Disease in Young Broilers Administered Natural Chicken Alpha Interferon via Oral and Intramuscular Routes

Comparative Study of Protection against Newcastle Disease in Young Broilers Administered Natural Chicken Alpha Interferon via Oral and Intramuscular Routes

Antiviral potential and stability analysis of chicken interferon-α produced by Newcastle disease virus in chicken embryo fibroblast cells

IBV QX affects the antigen presentation function of BMDCs through nonstructural protein16

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Antiviral potential and stability analysis of chicken interferon-α produced by Newcastle disease virus in chicken embryo fibroblast cells