Comprehensive profiling of the VOCs of Trichoderma longibrachiatum EF5 while interacting with Sclerotium rolfsii and Macrophomina phaseolina

Sridharan, A. P.; Binodh, Asish K.; Karthikeyan, G.; Uthandi, Sivakumar

doi:10.1016/j.micres.2020.126436

Cited by 47 publications

(16 citation statements)

References 54 publications

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“…Detailed lists of VOCs were given in Supplementary Table S1. Some representatives of a few genera have been already VOC profiled before, like Macrophomina [38], Oidiodendron [39], Phomopsis [40], Colletotrichum [41], Penicillium [42], Aspergillus [43], and Fusarium [2]. Numerous compounds were identified in those studies as well, such as methyl-1-propanol, methyl-1-butanol, acetone [40], methyl-1-butanol acetate, benzaldehyde [41], and styrene [42].…”

Section: Performance Of Proposed Cultivation Techniquementioning

confidence: 99%

Volatile Organic Compounds (VOCs) of Endophytic Fungi Growing on Extracts of the Host, Horseradish (Armoracia rusticana)

et al. 2020

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The interaction between plant defensive metabolites and different plant-associated fungal species is of high interest to many disciplines. Volatile organic compounds (VOCs) are natural products that are easily evaporated under ambient conditions. They play a very important role in inter-species communication of microbes and their hosts. In this study, the VOCs produced by 43 different fungal isolates of endophytic and soil fungi during growth on horseradish root (Armoracia rusticana) extract or malt extract agar were examined, by using headspace-gas chromatography-mass spectrometry (headspace-GC-MS) and a high relative surface agar film as a medium. The proposed technique enabled sensitive detection of several typical VOCs (acetone, methyl acetate, methyl formate, ethyl acetate, methyl butanol isomers, styrene, beta-phellandrene), along with glucosinolate decomposition products, including allyl cyanide and allyl isothiocyanate and other sulfur-containing compounds—carbon disulfide, dimethyl sulfide. The VOC patterns of fungi belonging to Setophoma, Paraphoma, Plectosphaerella, Pyrenochaeta, Volutella, Cadophora, Notophoma, and Curvularia genera were described for the first time. The VOC pattern was significantly different among the isolates. The pattern was indicative of putative myrosinase activity for many tested isolates. On the other hand, endophytes and soil fungi as groups could not be separated by VOC pattern or intensity.

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Section: Performance Of Proposed Cultivation Techniquementioning

confidence: 99%

Volatile Organic Compounds (VOCs) of Endophytic Fungi Growing on Extracts of the Host, Horseradish (Armoracia rusticana)

et al. 2020

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“…In addition to inhibiting the growth of the pathogen during direct interaction, the antibiosis via microbial volatile organic compounds (mVOCs) was observed in the case of Trichoderma longibrachiatum (Sridharan et al, 2020). These mVOCs reduced M. phaseolina mycelial growth by altering the mycelial structure.…”

Section: Fungal Bcasmentioning

confidence: 99%

Macrophomina phaseolina: General Characteristics of Pathogenicity and Methods of Control

et al. 2021

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Macrophomina phaseolina is a generalist soil-borne fungus present all over the world. It cause diseases such as stem and root rot, charcoal rot and seedling blight. Under high temperatures and low soil moisture, this fungus can cause substantial yield losses in crops such as soybean, sorghum and groundnut. The wide host range and high persistence of M. phaseolina in soil as microsclerotia make disease control challenging. Therefore, understanding the basis of the pathogenicity mechanisms as well as its interactions with host plants is crucial for controlling the pathogen. In this work, we aim to describe the general characteristics and pathogenicity mechanisms of M. phaseolina, as well as the hosts defense response. We also review the current methods and most promising forecoming ones to reach a responsible control of the pathogen, with minimal impacts to the environment and natural resources.

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“…A control was inoculated with only the phytopathogen. The behavior of the strains in the confrontation plate was evaluated in incubation at 28 • C for 96 h, and the percentage of inhibition was determined using formula 1 (Sridharan et al, 2020). The assay was carried out in triplicate with two biological replicates.…”

Section: Dual Culture Assaymentioning

confidence: 99%

“…Overlapping plates were separated by a layer of cellophane and sealed with parafilm. The assays were incubated at 28 • C for 96 h. A control was inoculated only with the respective phytopathogen (Sridharan et al, 2020). The percentage of inhibition was calculated using the formula 1 mentioned in the previous test.…”

Section: Evaluation Of the Effect Of Volatile Metabolites By The Over...mentioning

confidence: 99%

“…It has been reported that Trichoderma spp. can exert mycoparasitism, as well as the production of metabolites with antagonistic activity (De la Cruz‐Quiroz et al., 2019; Sridharan et al., 2020), siderophores (Zhao et al., 2020) and cell wall degrading enzymes (Manzar et al., 2021). In addition, this endophytic fungus works as a symbiont, favoring plants' development and increasing their resistance to biotic and abiotic stress (Rebolledo‐Prudencio et al., 2020).…”

Section: Introductionmentioning

confidence: 99%

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Evaluation of the biocontrol effect of Trichoderma sp. Trs GT2 extracts produced by fermentation processes against Botrytis cinerea Btcr GM3

Saldaña-Mendoza

Chávez‐González

Ramírez-Guzman

et al. 2023

Environmental Quality Mgmt

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In the present work, the evaluation of the use of Trichoderma spp. for controlling different phytopathogens of agricultural importance (Fusarium oxysporum, Rhizoctonia solani, Sclerotinia sclerotiorum, Botrytis cinerea, Colletotrichum gloeosporioides, and Phytophthora sp.) was carried out. Confrontation assays by dual culture, volatile metabolites (overlapping plate assay), and diffusible metabolites (poison medium assay) were performed to identify and select the Trichoderma strain with a greater spectrum of inhibition and the most sensitive phytopathogen. The production of hydrolytic enzymes associated with biological control by the Trichoderma sp. Trs GT2 strain (chitinase, cellulase, amylase, and lipase) was evaluated. Subsequently, the use of solid‐state and submerged fermentation to produce extracts with antagonistic activity against B. cinerea was evaluated, and the production of specific activity β 1–3 glucanase was compared. It was found that Trichoderma sp. Trs GT2 presented the greatest spectrum of inhibition against the phytopathogens evaluated, resulting in the strain of B. cinerea being the most sensitive microorganism to its effects (percentages of inhibition: dual culture 100%, volatile metabolites 100% and diffusible metabolites 28.24%). The Trichoderma sp. Trs GT2 strain showed amylase, cellulase, and chitinase activity. It also produced activity titers β 1–3 glucanase of 13.22 U/g by solid‐state fermentation (SSF) and 8.19 U/mL by submerged fermentation, as well as specific activities of (1.38 U/mg) and (14.23 U/mg), respectively. The extracts produced by both fermentation systems did not present an antagonistic effect against B. cinerea; this can be attributed to the need for interaction with the phytopathogen in question for the induction of metabolites with antagonistic activity, as well as to the confusion that the evaluation of the antagonistic effect of diffusible metabolites, through the cellophane paper technique, can generate in which the mechanism of nutrient competition can cause the inhibitory effect. This work shows that the ability of one microorganism to exert biocontrol against another can vary depending on the mechanism through which it performs and demonstrates that the antagonist‐phytopathogenic interaction can promote the production of metabolites with antagonistic activity.

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Comprehensive profiling of the VOCs of Trichoderma longibrachiatum EF5 while interacting with Sclerotium rolfsii and Macrophomina phaseolina

Cited by 47 publications

References 54 publications

Volatile Organic Compounds (VOCs) of Endophytic Fungi Growing on Extracts of the Host, Horseradish (Armoracia rusticana)

Volatile Organic Compounds (VOCs) of Endophytic Fungi Growing on Extracts of the Host, Horseradish (Armoracia rusticana)

Macrophomina phaseolina: General Characteristics of Pathogenicity and Methods of Control

Evaluation of the biocontrol effect of Trichoderma sp. Trs GT2 extracts produced by fermentation processes against Botrytis cinerea Btcr GM3

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